FastQCFastQC Report
Thu 26 May 2016
SRR1546049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546049_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences685663
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107441.5669505281749196No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13140.19163933302511585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACCG207.854256E-444.00000431
GGTACCC408.303687E-944.00000436
ATCTACG207.854256E-444.0000041
CGTTGAT207.854256E-444.00000425
GTCTAAT207.854256E-444.00000433
CCCACTC207.854256E-444.00000443
TATGACT207.854256E-444.00000413
ACGATAT207.854256E-444.00000434
TACGCGG207.854256E-444.0000042
TACGATA207.854256E-444.00000433
CTACGAT207.854256E-444.00000432
TCCACGT207.854256E-444.00000414
GCGATAC408.303687E-944.0000049
GCTAGAT207.854256E-444.00000418
CGGTCTA650.044.00000431
CCAACCG207.854256E-444.0000042
ATACGCA207.854256E-444.00000442
CACAACG207.854256E-444.00000412
GCCACTA207.854256E-444.0000049
AATACGC207.854256E-444.00000441
Produced by FastQC (version 0.11.5)