##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546049_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	685663
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.142914230460153	31.0	28.0	33.0	25.0	33.0
2	28.800060671204367	31.0	28.0	33.0	16.0	33.0
3	28.263372823092393	31.0	27.0	33.0	16.0	34.0
4	32.13720296997213	35.0	32.0	35.0	25.0	37.0
5	33.68592588487347	35.0	33.0	35.0	28.0	37.0
6	33.49134195661717	35.0	33.0	37.0	28.0	37.0
7	34.365449207555315	35.0	35.0	37.0	31.0	37.0
8	34.85258939158158	35.0	35.0	37.0	32.0	37.0
9	36.659953942388604	39.0	37.0	39.0	32.0	39.0
10	36.55163104907221	39.0	35.0	39.0	32.0	39.0
11	36.4533189044764	39.0	35.0	39.0	32.0	39.0
12	34.946469329685286	35.0	33.0	39.0	30.0	39.0
13	34.274366561999116	37.0	33.0	39.0	25.0	39.0
14	35.03272744774036	37.0	33.0	40.0	25.0	41.0
15	35.92842402171329	37.0	33.0	40.0	30.0	41.0
16	35.65958787334303	36.0	34.0	40.0	30.0	41.0
17	35.142615249765555	35.0	33.0	40.0	29.0	41.0
18	34.87393369629103	36.0	33.0	39.0	29.0	40.0
19	34.27550998085065	36.0	32.0	38.0	27.0	40.0
20	34.71288665131413	35.0	34.0	38.0	29.0	40.0
21	34.8336369324289	35.0	34.0	39.0	30.0	40.0
22	35.19477790109718	35.0	34.0	39.0	30.0	40.0
23	35.607315838830445	35.0	34.0	39.0	31.0	41.0
24	35.29168994097684	35.0	34.0	39.0	31.0	41.0
25	34.89723231383347	35.0	34.0	39.0	30.0	40.0
26	34.97521814652387	35.0	34.0	39.0	30.0	40.0
27	35.23777278342276	35.0	34.0	39.0	31.0	41.0
28	35.200546040839306	35.0	34.0	39.0	31.0	41.0
29	35.37994612513727	35.0	35.0	40.0	31.0	41.0
30	34.994282905742324	35.0	34.0	39.0	30.0	41.0
31	34.26134704658119	35.0	34.0	38.0	27.0	40.0
32	34.072475837255325	35.0	34.0	39.0	25.0	40.0
33	33.69999693727093	35.0	33.0	39.0	23.0	40.0
34	33.38827091442881	35.0	33.0	39.0	21.0	41.0
35	33.12777705665903	35.0	33.0	39.0	18.0	41.0
36	32.69579516468003	35.0	33.0	39.0	17.0	40.0
37	32.59274599912785	35.0	33.0	39.0	16.0	40.0
38	32.4826102618925	35.0	32.0	39.0	16.0	40.0
39	32.35218321536965	35.0	32.0	38.0	15.0	40.0
40	32.16157937645753	35.0	32.0	38.0	15.0	40.0
41	31.92644199847447	35.0	31.0	38.0	14.0	40.0
42	32.06721231858799	35.0	32.0	38.0	13.0	40.0
43	31.83738367098706	35.0	31.0	38.0	12.0	40.0
44	31.6188769118357	35.0	31.0	38.0	10.0	40.0
45	31.539380132805768	35.0	31.0	38.0	10.0	40.0
46	31.428051389676853	35.0	31.0	38.0	10.0	40.0
47	31.42481948129037	35.0	31.0	38.0	10.0	40.0
48	31.505287582967142	35.0	31.0	38.0	10.0	40.0
49	31.561593669193176	35.0	31.0	37.0	10.0	40.0
50	31.60822444845354	35.0	31.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	11.0
14	30.0
15	72.0
16	193.0
17	420.0
18	857.0
19	1423.0
20	2286.0
21	3452.0
22	4950.0
23	6857.0
24	10151.0
25	15258.0
26	20840.0
27	23519.0
28	22347.0
29	21575.0
30	23280.0
31	29180.0
32	40281.0
33	66232.0
34	113403.0
35	65151.0
36	51850.0
37	69821.0
38	75488.0
39	16730.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.01613328996898	22.310231119369135	28.04322239934195	23.630413191319935
2	37.70394494088204	20.3826077825404	30.114064781095085	11.799382495482474
3	27.002477893659133	18.53184436085949	41.87377764295288	12.591900102528502
4	24.600423239988157	23.921226608406755	37.13004785149556	14.348302300109527
5	18.969377084661122	29.427284248967787	38.73666801329516	12.866670653075928
6	18.580118804718936	35.36897863819398	36.173747161506455	9.877155395580628
7	75.8067738816299	5.057732442905626	16.872574427962427	2.2629192475020528
8	78.53231106243155	2.8938414352240094	16.104266964966754	2.4695805373776913
9	75.39345130187863	4.022092485667157	17.436699953183997	3.147756259270225
10	54.55508026537819	15.641211498943358	20.92514836005443	8.878559875624031
11	49.66244350358704	15.38598407672574	24.950595263270735	10.000977156416491
12	43.58628072391247	18.04953745498882	28.373559605812183	9.990622215286518
13	19.056154408215114	41.71101546969867	28.815467656851833	10.41736246523438
14	12.187036488770723	43.51598379962168	33.4731493459615	10.823830365646097
15	10.70963432473387	19.141764977838967	58.75145661935966	11.397144078067505
16	11.782172875012943	13.504009987413642	57.69773197620406	17.01608516136936
17	12.589129061944423	13.73575648678724	36.071364504136874	37.60374994713146
18	19.469331143725125	19.033986083542498	42.458029673469326	19.03865309926305
19	26.97462164357709	20.434236643948996	32.918649540663566	19.672492171810347
20	29.9476565018092	18.62576805223557	32.10221347804971	19.324361967905514
21	19.40793071815163	25.680254002330592	35.045204422580774	19.866610856937008
22	20.403025976317814	20.549891127273895	32.38208857704149	26.6649943193668
23	16.599991541034008	26.98833100225621	30.60337221054658	25.808305246163204
24	17.444138009488626	19.873028003552765	47.6712320775658	15.011601909392807
25	14.706933289385601	20.065688246266753	45.305200951487826	19.922177512859815
26	13.04387140621559	32.56628985376198	34.94194669976359	19.447892040258843
27	14.485833419624509	32.930900456930004	36.01390187307759	16.569364250367894
28	12.444889107331154	25.65239775224855	47.57657916498338	14.326133975436914
29	12.825105044314771	20.366856604483544	46.599714436975596	20.208323914226085
30	16.54705008145401	27.632379171692218	38.81031935513511	17.010251391718672
31	28.970645929560146	23.27615752928188	30.55013906248405	17.203057478673927
32	29.649842561141554	22.669153797127745	32.25097460414227	15.430029037588437
33	28.09441372802674	24.9600751389531	29.63102865401808	17.314482479002073
34	18.003742363230916	26.289445398103734	31.327634712679554	24.3791775259858
35	18.441712619756352	24.524584234529208	35.67029284065204	21.3634103050624
36	31.433517631839546	22.152865183041815	28.963791250220588	17.449825934898048
37	19.7216416811174	30.708671752741505	31.73570689974521	17.833979666395884
38	19.78172950851949	31.62121917035045	26.6200742930565	21.97697702807356
39	19.69553556193057	29.508519491353624	30.67921121600553	20.116733730710276
40	23.421564237825287	24.153848173227956	27.99290613610476	24.431681452841993
41	16.346514249711593	21.95772558822629	30.899290176077756	30.79646998598437
42	22.238184064183134	23.186463320902543	26.36805544414676	28.207297170767564
43	22.58412660446896	23.58826420559371	27.872292948576778	25.955316241360553
44	18.81171945985127	28.74298306894203	30.66404341491374	21.78125405629296
45	15.77145040639498	37.98557017076902	24.945636559067648	21.297342863768353
46	22.08461007812876	30.826513899685416	28.082892032966633	19.005983989219192
47	20.86185195934446	25.950357537157466	28.80073738848385	24.387053115014226
48	22.819810898356774	21.700602190872193	33.142957983732536	22.3366289270385
49	20.94221213628269	21.015134256916298	35.22998907626633	22.81266453053468
50	18.634518706711606	30.66141821857093	29.69490843169312	21.009154643024345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	463.0
2	637.0
3	8172.0
4	15707.0
5	11460.5
6	7214.0
7	7958.0
8	8702.0
9	9614.0
10	10526.0
11	10560.5
12	10595.0
13	10048.5
14	9502.0
15	8741.5
16	7981.0
17	7195.0
18	6409.0
19	5929.5
20	5450.0
21	4919.0
22	4388.0
23	4041.0
24	3694.0
25	3573.5
26	3453.0
27	3585.5
28	3718.0
29	4220.5
30	4723.0
31	5407.5
32	6092.0
33	7129.0
34	8166.0
35	9725.0
36	11284.0
37	13799.5
38	16315.0
39	22028.5
40	27742.0
41	42478.5
42	57215.0
43	66659.0
44	76103.0
45	75542.5
46	74982.0
47	70439.0
48	65896.0
49	60459.0
50	55022.0
51	49662.5
52	44303.0
53	39630.5
54	34958.0
55	30301.5
56	25645.0
57	22507.5
58	19370.0
59	17626.5
60	15883.0
61	14911.5
62	13940.0
63	12274.5
64	10609.0
65	8765.5
66	6922.0
67	5824.0
68	4726.0
69	3919.0
70	3112.0
71	2512.0
72	1912.0
73	1458.0
74	1004.0
75	884.5
76	765.0
77	572.5
78	380.0
79	256.0
80	132.0
81	124.5
82	117.0
83	75.5
84	34.0
85	27.0
86	20.0
87	14.0
88	8.0
89	9.5
90	11.0
91	6.5
92	2.0
93	1.5
94	1.0
95	2.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	685663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.669066270298067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76123336357715	20.217273187400732
2	7.332683799725452	3.7644629278858734
3	3.022993133227886	2.3279223321444777
4	1.8647845896848196	1.9146911684980092
5	1.26839062496439	1.6279201504417857
6	0.8483264467725876	1.3065448668631225
7	0.6345541777813758	1.140188926909525
8	0.48423382119873215	0.9943864037335939
9	0.34748300730936177	0.8027607908183829
>10	3.6421539560420753	21.908550335486563
>50	1.1999874601903053	21.98197106974701
>100	0.5920392677643456	20.25472348394932
>500	0.0	0.0
>1k	5.681758807719247E-4	0.19164091258449437
>5k	0.0	0.0
>10k+	5.681758807719247E-4	1.5669634435371442
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10744	1.5669505281749196	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1314	0.19163933302511585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4584424126721143E-4	0.0	0.0	0.038648723935811036	0.0
2	1.4584424126721143E-4	0.0	0.0	0.1454067085434098	0.0
3	1.4584424126721143E-4	0.0	0.0	0.19411868512665842	0.0
4	1.4584424126721143E-4	0.0	0.0	0.2930010807058278	0.0
5	1.4584424126721143E-4	0.0	0.0	0.47603560349617813	0.0
6	1.4584424126721143E-4	0.0	0.0	0.6632996092832777	0.0
7	1.4584424126721143E-4	0.0	0.0	0.7951428033888368	0.0
8	1.4584424126721143E-4	0.0	0.0	1.178129780956534	0.0
9	2.9168848253442286E-4	0.0	0.0	1.3547471571311271	0.0
10	2.9168848253442286E-4	0.0	0.0	1.6283509537484158	0.0
11	2.9168848253442286E-4	0.0	0.0	1.8427419884112166	0.0
12	2.9168848253442286E-4	0.0	0.0	2.049403278286855	0.0
13	4.375327238016343E-4	0.0	0.0	2.125679816469607	0.0
14	4.375327238016343E-4	0.0	0.0	2.1551403532055833	0.0
15	4.375327238016343E-4	0.0	0.0	2.19349738865886	0.0
16	4.375327238016343E-4	0.0	0.0	2.3005470617489934	0.0
17	4.375327238016343E-4	0.0	0.0	2.418097520210366	0.0
18	4.375327238016343E-4	0.0	0.0	2.650719085031568	0.0
19	4.375327238016343E-4	0.0	0.0	2.7194117226684247	0.0
20	5.833769650688457E-4	0.0	0.0	2.806626578946217	0.0
21	5.833769650688457E-4	0.0	0.0	2.9021545569762406	0.0
22	7.292212063360572E-4	0.0	0.0	2.994328117457118	0.0
23	8.750654476032686E-4	0.0	0.0	3.1054614293027334	0.0
24	8.750654476032686E-4	0.0	0.0	3.188009269859975	0.0
25	8.750654476032686E-4	0.0	0.0	3.247222031814463	0.0
26	8.750654476032686E-4	0.0	0.0	3.312268563419639	0.0
27	8.750654476032686E-4	0.0	0.0	3.3784818489549533	0.0
28	0.0010209096888704802	0.0	0.0	3.442799159353793	0.0
29	0.0010209096888704802	0.0	0.0	3.5176172551238727	0.0
30	0.0010209096888704802	0.0	0.0	3.6221875761124633	0.0
31	0.0010209096888704802	0.0	0.0	3.721945037139236	0.0
32	0.0010209096888704802	0.0	0.0	3.8079931394868907	0.0
33	0.0011667539301376915	0.0	0.0	3.8902492915615983	0.0
34	0.0011667539301376915	0.0	0.0	3.978339213286994	0.0
35	0.0011667539301376915	0.0	0.0	4.099244089297512	0.0
36	0.0011667539301376915	0.0	0.0	4.183833749232495	0.0
37	0.001312598171404903	0.0	0.0	4.2745488673007	0.0
38	0.001312598171404903	0.0	0.0	4.366139050816509	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACCG	20	7.854256E-4	44.000004	31
GGTACCC	40	8.303687E-9	44.000004	36
ATCTACG	20	7.854256E-4	44.000004	1
CGTTGAT	20	7.854256E-4	44.000004	25
GTCTAAT	20	7.854256E-4	44.000004	33
CCCACTC	20	7.854256E-4	44.000004	43
TATGACT	20	7.854256E-4	44.000004	13
ACGATAT	20	7.854256E-4	44.000004	34
TACGCGG	20	7.854256E-4	44.000004	2
TACGATA	20	7.854256E-4	44.000004	33
CTACGAT	20	7.854256E-4	44.000004	32
TCCACGT	20	7.854256E-4	44.000004	14
GCGATAC	40	8.303687E-9	44.000004	9
GCTAGAT	20	7.854256E-4	44.000004	18
CGGTCTA	65	0.0	44.000004	31
CCAACCG	20	7.854256E-4	44.000004	2
ATACGCA	20	7.854256E-4	44.000004	42
CACAACG	20	7.854256E-4	44.000004	12
GCCACTA	20	7.854256E-4	44.000004	9
AATACGC	20	7.854256E-4	44.000004	41
>>END_MODULE