FastQCFastQC Report
Thu 26 May 2016
SRR1546048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546048_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences689956
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110601.6030007710636622No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13180.19102667416472935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTA207.854291E-444.029
ACGTTAG254.4409855E-544.01
CTATCGC254.4409855E-544.020
GCGCGAT207.854291E-444.018
ATAATCG254.4409855E-544.016
AGCTTCA254.4409855E-544.042
CGTTGAT254.4409855E-544.025
ATTACCG302.5262398E-644.01
ACACACG254.4409855E-544.029
GTTAGCG408.303687E-944.01
CGATTTA254.4409855E-544.010
GTCTAGA207.854291E-444.011
CGCGATC207.854291E-444.019
GAATACC207.854291E-444.041
ACGGGTA254.4409855E-544.05
ATCACAC207.854291E-444.026
TATGACT254.4409855E-544.013
ATGTACG254.4409855E-544.01
CCGTATG254.4409855E-544.035
ACCTAGC207.854291E-444.034
Produced by FastQC (version 0.11.5)