##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546048_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	689956
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.108425754685808	31.0	28.0	33.0	25.0	33.0
2	28.7594324855498	31.0	28.0	33.0	16.0	33.0
3	28.248246264979215	31.0	27.0	33.0	16.0	34.0
4	32.12086133028773	35.0	32.0	35.0	25.0	37.0
5	33.71416293212901	35.0	33.0	35.0	30.0	37.0
6	33.57553090341993	35.0	33.0	37.0	28.0	37.0
7	34.42569671109462	35.0	35.0	37.0	32.0	37.0
8	34.88402303915032	35.0	35.0	37.0	32.0	37.0
9	36.765835792427346	39.0	37.0	39.0	32.0	39.0
10	36.571595869881556	39.0	35.0	39.0	32.0	39.0
11	36.504368394506315	39.0	35.0	39.0	32.0	39.0
12	34.90828545588414	35.0	33.0	39.0	30.0	39.0
13	34.318810184997304	37.0	33.0	39.0	25.0	39.0
14	35.069794305723846	37.0	33.0	40.0	25.0	41.0
15	35.9892326467195	37.0	34.0	40.0	30.0	41.0
16	35.71177292465027	36.0	34.0	40.0	31.0	41.0
17	35.19489793552052	35.0	33.0	40.0	29.0	41.0
18	34.95007797598687	36.0	33.0	39.0	29.0	40.0
19	34.33853318182609	36.0	32.0	38.0	27.0	40.0
20	34.68340589834714	35.0	34.0	38.0	29.0	40.0
21	34.77107380760512	35.0	34.0	38.0	29.0	40.0
22	35.17721419916632	35.0	34.0	39.0	30.0	40.0
23	35.62821397306495	35.0	34.0	39.0	31.0	41.0
24	35.184320449419964	35.0	34.0	39.0	31.0	40.0
25	34.79643774385613	35.0	34.0	39.0	30.0	40.0
26	34.929901327041144	35.0	34.0	39.0	30.0	40.0
27	35.246079460139484	35.0	34.0	39.0	31.0	41.0
28	35.2136136217382	35.0	34.0	39.0	31.0	41.0
29	35.38498976746343	35.0	35.0	40.0	31.0	41.0
30	35.02651763300848	35.0	34.0	39.0	30.0	41.0
31	34.311141580042786	35.0	34.0	38.0	28.0	40.0
32	34.14807900793674	35.0	34.0	39.0	26.0	40.0
33	33.78990399387787	35.0	33.0	39.0	23.0	40.0
34	33.41617146600653	35.0	33.0	39.0	21.0	41.0
35	33.17226460817791	35.0	33.0	39.0	18.0	41.0
36	32.724843323342355	35.0	33.0	39.0	17.0	40.0
37	32.61867858240235	35.0	33.0	39.0	16.0	40.0
38	32.53021207149441	35.0	32.0	39.0	17.0	40.0
39	32.3665451130217	35.0	32.0	38.0	15.0	40.0
40	32.152008244003966	35.0	32.0	38.0	15.0	40.0
41	31.8988674640122	35.0	31.0	38.0	14.0	40.0
42	32.047510276017604	35.0	32.0	38.0	13.0	40.0
43	31.83207769770826	35.0	31.0	38.0	12.0	40.0
44	31.611544794160785	35.0	31.0	38.0	10.0	40.0
45	31.525830632678026	35.0	31.0	38.0	10.0	40.0
46	31.39725286829885	35.0	31.0	38.0	10.0	40.0
47	31.39434108841723	35.0	31.0	38.0	10.0	40.0
48	31.473644696183523	35.0	31.0	38.0	10.0	40.0
49	31.58494309782073	35.0	31.0	37.0	10.0	40.0
50	31.652934390019073	35.0	31.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	6.0
13	15.0
14	17.0
15	46.0
16	186.0
17	431.0
18	859.0
19	1367.0
20	2257.0
21	3417.0
22	4821.0
23	6792.0
24	10091.0
25	15037.0
26	20848.0
27	23773.0
28	22204.0
29	21636.0
30	23702.0
31	29042.0
32	40422.0
33	66658.0
34	115294.0
35	65343.0
36	53094.0
37	71992.0
38	76053.0
39	14550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.151377769017152	22.16242774901588	27.94279635223116	23.74339812973581
2	37.884879615511714	20.263611012876183	30.02075494669225	11.83075442491985
3	26.955631953341953	18.444509504953942	41.91557142774322	12.68428711396089
4	24.449819988521007	24.04501156595493	37.180052061290866	14.325116384233198
5	18.882943260149922	29.41245528700381	38.74145597690288	12.963145475943394
6	18.48567155006986	35.399938546805885	36.312025694392105	9.80236420873215
7	75.65569398628318	5.0503220495219985	17.017317046304402	2.276666917890416
8	78.38065036031283	2.9068520311440147	16.271762257303365	2.440735351239789
9	75.32349888978428	4.032865863910162	17.575758454162294	3.067876792143267
10	54.50738887697186	15.497365049365467	20.941915136617407	9.05333093704526
11	49.53011496385277	15.372864356567664	25.163198812677912	9.933821866901656
12	43.604229834946	17.720985106296634	28.41210164126408	10.262683417493289
13	19.045562325713526	41.581347216344234	28.94633860709958	10.426751850842662
14	12.308755920667405	43.453205711668566	33.4559015357501	10.782136831913919
15	10.744163395926696	19.119480082787888	58.78925612647763	11.347100394807786
16	11.780171489196412	13.529558406623032	57.78122083147331	16.909049272707243
17	12.580512380499625	13.680582529900457	36.12491231324896	37.613992776350955
18	19.526317620254044	18.94077303480222	42.532422357367714	19.00048698757602
19	27.04418832505261	20.42796931978271	32.9032575990353	19.624584756129376
20	29.941184655253377	18.34348857028564	32.205966757300466	19.509360017160514
21	19.40341123202059	25.595400286395076	35.20123022337656	19.79995825820777
22	20.413765515482147	20.510003536457397	32.49468082022622	26.58155012783424
23	16.5726510096296	26.865771150624095	30.6823913408971	25.8791864988492
24	17.479375496408466	19.82358295311585	47.635501394291815	15.061540156183872
25	14.673254526375596	20.043162172660285	45.320281293299864	19.963302007664257
26	12.996191061458992	32.39481937978654	35.124558667509234	19.48443089124524
27	14.45773353663132	32.75005942407921	36.1570882780931	16.635118761196367
28	12.45064902689447	25.665259813669277	47.662604571885744	14.22148658755051
29	12.879516954704359	20.296076851277473	46.651960414867034	20.172445779151136
30	16.582941520908577	27.497550568442048	38.94697632892532	16.972531581724052
31	28.926337331655933	23.263947266202482	30.647026766924267	17.162688635217318
32	29.550580036987867	22.607818469583567	32.477143470018376	15.364458023410188
33	27.911490008058486	24.943184782797744	29.822626370377243	17.322698838766527
34	18.11767706926239	26.272979726243413	31.44490373299167	24.16443947150253
35	18.30189171483399	24.47025607430039	35.785615314599774	21.442236896265847
36	31.29199543159274	22.13662900242914	29.097362730377007	17.474012835601112
37	19.696183524746505	30.544121654134464	32.02291160595748	17.736783215161548
38	19.79053736760025	31.447947405341793	26.7533002104482	22.008215016609757
39	19.631396784722504	29.463617969841554	30.744859092463866	20.160126152972076
40	23.358446045834807	24.204442022389834	27.998886885540525	24.438225046234834
41	16.302198980804572	21.985459942373136	31.033718092168193	30.67862298465409
42	22.305770223028716	23.159447848848334	26.451107027114773	28.08367490100818
43	22.561003890103137	23.670929740447217	27.95830458753892	25.80976178191073
44	18.88468250149285	28.79560435737931	30.600357124222416	21.719356016905426
45	15.79521012934158	37.899228356590854	25.135226014412517	21.17033549965505
46	22.117787221214105	30.79326797650865	28.21716167407777	18.87178312819948
47	20.876838523036252	26.063835954756538	28.802851196308172	24.256474325899045
48	22.895807848616435	21.72834209717721	33.10037161790027	22.275478436306084
49	20.94177019983883	21.053371519343262	35.16021311503922	22.844645165778687
50	18.574952605673406	30.664853990689263	29.69812567757944	21.062067726057894
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	321.0
1	490.0
2	659.0
3	8410.5
4	16162.0
5	11754.5
6	7347.0
7	8069.5
8	8792.0
9	9719.0
10	10646.0
11	10762.0
12	10878.0
13	10211.0
14	9544.0
15	8816.5
16	8089.0
17	7338.0
18	6587.0
19	5955.5
20	5324.0
21	4901.0
22	4478.0
23	4114.0
24	3750.0
25	3618.0
26	3486.0
27	3527.5
28	3569.0
29	4189.5
30	4810.0
31	5514.5
32	6219.0
33	7185.0
34	8151.0
35	9807.0
36	11463.0
37	13889.5
38	16316.0
39	21912.0
40	27508.0
41	42453.0
42	57398.0
43	66829.0
44	76260.0
45	75883.5
46	75507.0
47	70639.5
48	65772.0
49	60541.0
50	55310.0
51	49914.5
52	44519.0
53	39995.5
54	35472.0
55	30446.0
56	25420.0
57	22542.5
58	19665.0
59	17923.0
60	16181.0
61	15207.0
62	14233.0
63	12477.0
64	10721.0
65	8803.5
66	6886.0
67	5872.5
68	4859.0
69	4002.0
70	3145.0
71	2571.0
72	1997.0
73	1527.5
74	1058.0
75	910.0
76	762.0
77	575.5
78	389.0
79	259.0
80	129.0
81	118.5
82	108.0
83	71.5
84	35.0
85	26.0
86	17.0
87	11.5
88	6.0
89	3.0
90	0.0
91	1.0
92	2.0
93	2.0
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	689956.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.455636325936776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.28968357237088	19.9291371309094
2	7.556691099380183	3.8472076090653062
3	3.1678722866709057	2.4192061456952496
4	1.8572730108189974	1.8911226528554415
5	1.2456641962720003	1.5854587382270184
6	0.8947731713145042	1.3666212265912285
7	0.6566287065000567	1.1700431087685004
8	0.5141396668786005	1.0470201904639949
9	0.3577801620401694	0.8196769520576355
>10	3.6505463260424817	21.924325804062406
>50	1.2076782198924767	21.971164919019273
>100	0.600127569317727	20.229832690079487
>500	0.0	0.0
>1k	5.71006250540178E-4	0.19157561583828336
>5k	0.0	0.0
>10k+	5.71006250540178E-4	1.6076072163667785
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11060	1.6030007710636622	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1318	0.19102667416472935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.033625332629906836	0.0
2	0.0	0.0	0.0	0.12942854326942588	0.0
3	0.0	0.0	0.0	0.17305451362115845	0.0
4	0.0	0.0	0.0	0.2698722817107178	0.0
5	0.0	0.0	0.0	0.44814451936065486	0.0
6	0.0	0.0	0.0	0.6385914464110755	0.0
7	0.0	0.0	0.0	0.7628022656517227	0.0
8	0.0	0.0	0.0	1.1490587805599197	0.0
9	0.0	0.0	0.0	1.3163158230379908	0.0
10	0.0	0.0	0.0	1.585753294412977	0.0
11	0.0	0.0	0.0	1.7877951637495724	0.0
12	0.0	0.0	0.0	1.9815756367072683	0.0
13	0.0	0.0	0.0	2.0531744053243974	0.0
14	0.0	0.0	0.0	2.080422519696908	0.0
15	0.0	0.0	0.0	2.1201351970270568	0.0
16	1.4493677857718463E-4	0.0	0.0	2.221735878809663	0.0
17	1.4493677857718463E-4	0.0	0.0	2.343337836035921	0.0
18	1.4493677857718463E-4	0.0	0.0	2.565380980816168	0.0
19	1.4493677857718463E-4	0.0	0.0	2.6295879737258607	0.0
20	1.4493677857718463E-4	0.0	0.0	2.7175645983222116	0.0
21	2.8987355715436925E-4	0.0	0.0	2.817281101983315	0.0
22	2.8987355715436925E-4	0.0	0.0	2.902938738122431	0.0
23	2.8987355715436925E-4	0.0	0.0	3.004249546347883	0.0
24	2.8987355715436925E-4	0.0	0.0	3.088892625036959	0.0
25	2.8987355715436925E-4	0.0	0.0	3.150200882375108	0.0
26	2.8987355715436925E-4	0.0	0.0	3.2126686339418744	0.0
27	2.8987355715436925E-4	0.0	0.0	3.2774553739658763	0.0
28	4.348103357315539E-4	0.0	0.0	3.337749073853985	0.0
29	4.348103357315539E-4	0.0	0.0	3.4090579689139595	0.0
30	4.348103357315539E-4	0.0	0.0	3.5106586506965662	0.0
31	4.348103357315539E-4	0.0	0.0	3.608346039457589	0.0
32	4.348103357315539E-4	0.0	0.0	3.686466963110691	0.0
33	4.348103357315539E-4	0.0	0.0	3.763863202870908	0.0
34	4.348103357315539E-4	0.0	0.0	3.8474917241099433	0.0
35	4.348103357315539E-4	0.0	0.0	3.9717025433505904	0.0
36	4.348103357315539E-4	0.0	0.0	4.05822980016117	0.0
37	4.348103357315539E-4	0.0	0.0	4.139974143278701	0.0
38	4.348103357315539E-4	0.0	0.0	4.234617859689603	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGTA	20	7.854291E-4	44.0	29
ACGTTAG	25	4.4409855E-5	44.0	1
CTATCGC	25	4.4409855E-5	44.0	20
GCGCGAT	20	7.854291E-4	44.0	18
ATAATCG	25	4.4409855E-5	44.0	16
AGCTTCA	25	4.4409855E-5	44.0	42
CGTTGAT	25	4.4409855E-5	44.0	25
ATTACCG	30	2.5262398E-6	44.0	1
ACACACG	25	4.4409855E-5	44.0	29
GTTAGCG	40	8.303687E-9	44.0	1
CGATTTA	25	4.4409855E-5	44.0	10
GTCTAGA	20	7.854291E-4	44.0	11
CGCGATC	20	7.854291E-4	44.0	19
GAATACC	20	7.854291E-4	44.0	41
ACGGGTA	25	4.4409855E-5	44.0	5
ATCACAC	20	7.854291E-4	44.0	26
TATGACT	25	4.4409855E-5	44.0	13
ATGTACG	25	4.4409855E-5	44.0	1
CCGTATG	25	4.4409855E-5	44.0	35
ACCTAGC	20	7.854291E-4	44.0	34
>>END_MODULE