FastQCFastQC Report
Thu 26 May 2016
SRR1546047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546047_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences679440
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108781.6010243730130695No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13300.19574944071588368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA650.044.00000431
GTTTGCG207.8542065E-444.01
CTAGAAT207.8542065E-444.041
GTACACT700.044.018
ATCTAAC207.8542065E-444.024
CACGGCA207.8542065E-444.017
CGACGAT502.7284841E-1144.034
GACACAA254.4409113E-544.09
AGCCCGA254.4409113E-544.032
ATAATCG254.4409113E-544.016
CGTTGCC207.8542065E-444.021
CCCAGTA207.8542065E-444.041
TTGTACG302.5261816E-644.01
TAAGTCG207.8542065E-444.01
ACGGGTC207.8542065E-444.05
TCGATAG207.8542065E-444.01
TAGCCGT207.8542065E-444.044
CCGTCGA207.8542065E-444.015
TAGTTCG207.8542065E-444.01
CCGTATG207.8542065E-444.035
Produced by FastQC (version 0.11.5)