##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546047_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	679440
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.00545302013423	31.0	28.0	33.0	23.0	33.0
2	28.64303691275168	31.0	28.0	33.0	16.0	33.0
3	28.131630460379135	31.0	26.0	33.0	16.0	34.0
4	32.017150888967386	35.0	32.0	35.0	25.0	37.0
5	33.65821117390792	35.0	33.0	35.0	28.0	37.0
6	33.50109207582715	35.0	33.0	37.0	28.0	37.0
7	34.34860178970917	35.0	35.0	37.0	31.0	37.0
8	34.85490992582127	35.0	35.0	37.0	32.0	37.0
9	36.7559740374426	39.0	37.0	39.0	32.0	39.0
10	36.61899505475097	39.0	35.0	39.0	32.0	39.0
11	36.50143058989756	39.0	35.0	39.0	32.0	39.0
12	35.055095372659835	35.0	34.0	39.0	30.0	39.0
13	34.38988873189685	37.0	33.0	39.0	25.0	39.0
14	35.11482985988461	37.0	33.0	40.0	25.0	41.0
15	35.95947250677028	37.0	34.0	40.0	30.0	41.0
16	35.657381078535266	36.0	34.0	40.0	30.0	41.0
17	35.147069645590484	35.0	33.0	39.0	29.0	41.0
18	34.87887377840575	36.0	33.0	39.0	29.0	40.0
19	34.24428058401036	36.0	32.0	38.0	27.0	40.0
20	34.77443041328153	35.0	34.0	38.0	29.0	40.0
21	34.803374838101966	35.0	34.0	39.0	29.0	40.0
22	35.16928352761097	35.0	34.0	39.0	30.0	40.0
23	35.607866772636285	35.0	34.0	39.0	31.0	41.0
24	35.18741610738255	35.0	34.0	39.0	31.0	41.0
25	34.813065171317554	35.0	34.0	39.0	30.0	40.0
26	34.93555869539621	35.0	34.0	39.0	30.0	40.0
27	35.24377134110444	35.0	34.0	39.0	31.0	41.0
28	35.223235311432944	35.0	34.0	39.0	31.0	41.0
29	35.39480896032026	35.0	35.0	40.0	31.0	41.0
30	35.02304103379254	35.0	34.0	39.0	30.0	41.0
31	34.270656717296596	35.0	34.0	38.0	27.0	40.0
32	34.11772047568586	35.0	34.0	39.0	26.0	40.0
33	33.782255975509244	35.0	33.0	39.0	23.0	41.0
34	33.40544124573178	35.0	33.0	39.0	21.0	41.0
35	33.13067379018015	35.0	33.0	39.0	18.0	41.0
36	32.7287427881785	35.0	33.0	39.0	17.0	40.0
37	32.63187183562934	35.0	33.0	39.0	16.0	40.0
38	32.53289032144119	35.0	32.0	39.0	16.0	40.0
39	32.35451253973861	35.0	32.0	38.0	15.0	40.0
40	32.1840221358766	35.0	32.0	38.0	15.0	40.0
41	31.92724596726716	35.0	31.0	38.0	14.0	40.0
42	32.10280819498411	35.0	32.0	38.0	13.0	40.0
43	31.885480689979982	35.0	32.0	38.0	12.0	40.0
44	31.66970446249853	35.0	31.0	38.0	10.0	40.0
45	31.595955492758744	35.0	31.0	38.0	10.0	40.0
46	31.47583009537266	35.0	31.0	38.0	10.0	40.0
47	31.488587660426234	35.0	31.0	38.0	10.0	40.0
48	31.55560167196515	35.0	31.0	38.0	10.0	40.0
49	31.671959260567526	35.0	31.0	38.0	10.0	40.0
50	31.669003885552808	35.0	31.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	12.0
14	20.0
15	71.0
16	169.0
17	387.0
18	857.0
19	1460.0
20	2212.0
21	3219.0
22	4774.0
23	6821.0
24	9958.0
25	15067.0
26	20938.0
27	23492.0
28	22037.0
29	21281.0
30	23120.0
31	28439.0
32	39650.0
33	65354.0
34	113150.0
35	64105.0
36	51510.0
37	69581.0
38	74972.0
39	16775.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.034234075120686	22.214912280701753	27.942128811962792	23.808724832214764
2	37.92696926880961	20.3031908630637	30.00353232073472	11.76630754739197
3	26.934681502413753	18.558371600141292	41.798981514188156	12.7079653832568
4	24.509448957965382	24.068350406216883	37.04227010479218	14.37993053102555
5	18.836541858000707	29.427764040974917	38.756181561285764	12.979512539738607
6	18.534234075120686	35.30142470269634	36.29665606970446	9.867685152478511
7	75.52072294831036	5.181914517838219	16.95881902743436	2.3385435064170497
8	78.31169786883315	2.956405274932297	16.280466266336983	2.4514305898975626
9	75.26963381608384	4.066731425880136	17.594195219592606	3.069439538443424
10	54.49944071588367	15.642440833627694	21.010096550100084	8.848021900388556
11	49.63852584481337	15.273166136818556	25.122159425409162	9.966148592958907
12	43.67273048392794	18.034557871188035	28.35747085835394	9.935240786530084
13	19.047009301777933	41.6582774049217	28.883492287766394	10.41122100553397
14	12.245378547038738	43.459466619569056	33.548216178028966	10.746938655363241
15	10.758124337689862	19.18300365006476	58.76957494407159	11.28929706817379
16	11.699634993524079	13.531437654539033	57.74608501118568	17.022842340751208
17	12.530024726245143	13.721005533969151	36.15065347933593	37.598316260449785
18	19.570675850700578	18.905127752266573	42.456581890969034	19.06761450606382
19	27.04565524549629	20.45949605557518	32.89532556222772	19.599523136700814
20	30.042829388908515	18.566613681855646	32.11644884022136	19.274108089014483
21	19.38464029200518	25.63257977157659	35.0853644177558	19.897415518662427
22	20.323648887318967	20.59151654303544	32.43421052631579	26.650624043329803
23	16.530525138349226	26.99811609560815	30.703520546332275	25.76783821971035
24	17.440539267632168	19.897415518662427	47.60699988225598	15.05504533144943
25	14.681060873660662	20.13157894736842	45.362945955492755	19.824414223478158
26	12.935505710585188	32.5547509713882	35.054603791357586	19.455139526669022
27	14.431443541740258	32.93329801012599	36.09663840810079	16.53862004003297
28	12.453049570234311	25.55854821617803	47.709878723654775	14.278523489932887
29	12.912692805840104	20.255504533144943	46.79603791357589	20.035764747439067
30	16.467532085246674	27.532526786765573	38.99623219121629	17.00370893677146
31	29.074384787472034	23.243700694689746	30.48657718120805	17.195337336630164
32	29.7044624985282	22.643500529848108	32.27319557282468	15.378841398799011
33	27.97141763805487	24.966001412928293	29.73934416578359	17.323236783233252
34	18.035146591310493	26.314464853408694	31.457671023195573	24.192717532085247
35	18.31478864947604	24.5238726009655	35.83539385376192	21.32594489579654
36	31.37966560697045	22.14588484634405	29.154597904156365	17.31985164252914
37	19.63057812316025	30.683209702107618	31.917461438831978	17.768750735900152
38	19.684887554456612	31.63193217944189	26.71935711762628	21.963823148475214
39	19.801601318733077	29.48501707288355	30.72338985046509	19.989991757918286
40	23.530260214294124	24.195513952666904	27.989079241728483	24.285146591310493
41	16.295184269398327	21.96661956905687	31.06087366066172	30.67732250088308
42	22.310579300600494	23.101524785117157	26.435299658542327	28.15259625574002
43	22.507800541622515	23.712174732132343	27.899005062993055	25.88101966325209
44	18.841693159072175	28.740433298010124	30.78108442246556	21.63678912045214
45	15.806988107853526	37.93727187095255	25.135552808194983	21.12018721299894
46	22.104821617802898	30.941805015895447	28.13670081243377	18.816672553867893
47	20.788149063935006	26.106205110090663	28.821529494878135	24.284116331096197
48	22.92108206758507	21.65754150476863	33.24016837395502	22.181208053691275
49	20.918256210997292	20.967267161191568	35.315259625574	22.79921700223714
50	18.49552572706935	30.77313670081243	29.770399152243023	20.96093841987519
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	338.0
1	506.5
2	675.0
3	8380.5
4	16086.0
5	11750.0
6	7414.0
7	8121.0
8	8828.0
9	9692.0
10	10556.0
11	10581.5
12	10607.0
13	9954.5
14	9302.0
15	8544.5
16	7787.0
17	7049.5
18	6312.0
19	5788.0
20	5264.0
21	4799.5
22	4335.0
23	4011.5
24	3688.0
25	3545.5
26	3403.0
27	3496.5
28	3590.0
29	4106.0
30	4622.0
31	5378.0
32	6134.0
33	7051.5
34	7969.0
35	9683.0
36	11397.0
37	13742.0
38	16087.0
39	21621.0
40	27155.0
41	41903.5
42	56652.0
43	66002.5
44	75353.0
45	75132.0
46	74911.0
47	69939.0
48	64967.0
49	59720.5
50	54474.0
51	48860.0
52	43246.0
53	39138.0
54	35030.0
55	29997.5
56	24965.0
57	22129.5
58	19294.0
59	17450.0
60	15606.0
61	14781.5
62	13957.0
63	12252.5
64	10548.0
65	8691.5
66	6835.0
67	5785.5
68	4736.0
69	3864.5
70	2993.0
71	2466.5
72	1940.0
73	1477.5
74	1015.0
75	860.0
76	705.0
77	529.5
78	354.0
79	246.0
80	138.0
81	121.5
82	105.0
83	63.5
84	22.0
85	23.5
86	25.0
87	18.0
88	11.0
89	7.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	679440.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.451537275435125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.57453112936676	19.99842607938916
2	7.327142161484912	3.729740636908911
3	3.013465910527759	2.300920198501509
4	1.9104215851970374	1.9449266474975302
5	1.2102168228964547	1.540093928965389
6	0.899540198474888	1.3736808535341554
7	0.6306947458788452	1.123650558390956
8	0.509537041222789	1.037480079831739
9	0.3930886862969181	0.9004240216654052
>10	3.7350167682043023	22.29679174667775
>50	1.2091339602058273	22.132771751724533
>100	0.5860539242528723	19.823519932958927
>500	0.0	0.0
>1k	5.78532995313793E-4	0.19583656946746522
>5k	0.0	0.0
>10k+	5.78532995313793E-4	1.6017369944865312
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10878	1.6010243730130695	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1330	0.19574944071588368	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03767808783704227	0.0
2	0.0	0.0	0.0	0.13687742847050513	0.0
3	0.0	0.0	0.0	0.18044271753208524	0.0
4	0.0	0.0	0.0	0.26772047568585894	0.0
5	0.0	0.0	0.0	0.44992935358530556	0.0
6	0.0	0.0	0.0	0.6325797715765925	0.0
7	0.0	0.0	0.0	0.7706346402920052	0.0
8	0.0	0.0	0.0	1.1440303779583185	0.0
9	0.0	0.0	0.0	1.3040150712351348	0.0
10	0.0	0.0	0.0	1.568203226186271	0.0
11	0.0	0.0	0.0	1.7633639467797009	0.0
12	1.4718003061344636E-4	0.0	0.0	1.9471918050158954	0.0
13	4.415400918403391E-4	0.0	0.0	2.0253444012716355	0.0
14	4.415400918403391E-4	0.0	0.0	2.050659366537148	0.0
15	4.415400918403391E-4	0.0	0.0	2.0878959142823503	0.0
16	4.415400918403391E-4	0.0	0.0	2.187095254915813	0.0
17	4.415400918403391E-4	0.0	0.0	2.3016013187330744	0.0
18	4.415400918403391E-4	0.0	0.0	2.509861062051101	0.0
19	4.415400918403391E-4	0.0	0.0	2.570940774755681	0.0
20	4.415400918403391E-4	0.0	0.0	2.657924172848228	0.0
21	4.415400918403391E-4	0.0	0.0	2.7506475921346993	0.0
22	4.415400918403391E-4	0.0	0.0	2.834098669492523	0.0
23	4.415400918403391E-4	0.0	0.0	2.937566231013776	0.0
24	4.415400918403391E-4	0.0	0.0	3.0098316260449782	0.0
25	4.415400918403391E-4	0.0	0.0	3.067820558106676	0.0
26	4.415400918403391E-4	0.0	0.0	3.1352290121276347	0.0
27	4.415400918403391E-4	0.0	0.0	3.19439538443424	0.0
28	5.887201224537854E-4	0.0	0.0	3.255622277169434	0.0
29	5.887201224537854E-4	0.0	0.0	3.3237666313434593	0.0
30	5.887201224537854E-4	0.0	0.0	3.4133992699870483	0.0
31	7.359001530672318E-4	0.0	0.0	3.499941127987755	0.0
32	7.359001530672318E-4	0.0	0.0	3.575444483692453	0.0
33	7.359001530672318E-4	0.0	0.0	3.6547745201931003	0.0
34	7.359001530672318E-4	0.0	0.0	3.742788178499941	0.0
35	7.359001530672318E-4	0.0	0.0	3.8612681031437655	0.0
36	7.359001530672318E-4	0.0	0.0	3.940156599552573	0.0
37	7.359001530672318E-4	0.0	0.0	4.0199281761450605	0.0
38	7.359001530672318E-4	0.0	0.0	4.112357235370305	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	65	0.0	44.000004	31
GTTTGCG	20	7.8542065E-4	44.0	1
CTAGAAT	20	7.8542065E-4	44.0	41
GTACACT	70	0.0	44.0	18
ATCTAAC	20	7.8542065E-4	44.0	24
CACGGCA	20	7.8542065E-4	44.0	17
CGACGAT	50	2.7284841E-11	44.0	34
GACACAA	25	4.4409113E-5	44.0	9
AGCCCGA	25	4.4409113E-5	44.0	32
ATAATCG	25	4.4409113E-5	44.0	16
CGTTGCC	20	7.8542065E-4	44.0	21
CCCAGTA	20	7.8542065E-4	44.0	41
TTGTACG	30	2.5261816E-6	44.0	1
TAAGTCG	20	7.8542065E-4	44.0	1
ACGGGTC	20	7.8542065E-4	44.0	5
TCGATAG	20	7.8542065E-4	44.0	1
TAGCCGT	20	7.8542065E-4	44.0	44
CCGTCGA	20	7.8542065E-4	44.0	15
TAGTTCG	20	7.8542065E-4	44.0	1
CCGTATG	20	7.8542065E-4	44.0	35
>>END_MODULE