FastQCFastQC Report
Thu 26 May 2016
SRR1546046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546046_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences597523
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56410.9440640778681323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTC302.5256686E-644.0000049
GCGTTAG600.044.0000041
TTCGCAA302.5256686E-644.00000414
CGTCAGA302.5256686E-644.00000430
ACGCGAG207.853443E-444.01
CGGAATC408.301868E-944.09
AGCGTAA254.440266E-544.01
TTGGCCA207.853443E-444.05
GTACACT551.8189894E-1244.018
CACGGTA207.853443E-444.030
ACGTTAG408.301868E-944.01
CCGGCAT207.853443E-444.017
GACAATC207.853443E-444.09
AGAAGTC207.853443E-444.036
GTCTAAA408.301868E-944.033
ACTTATG254.440266E-544.01
ATACTCG351.4445322E-744.044
ACCTAGC207.853443E-444.034
ATTCACG207.853443E-444.01
TCTAACG254.440266E-544.023
Produced by FastQC (version 0.11.5)