##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546046_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	597523
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.085263663490778	28.0	25.0	30.0	16.0	33.0
2	26.08341101514084	30.0	25.0	31.0	10.0	33.0
3	26.408739077826294	30.0	25.0	31.0	16.0	33.0
4	27.956671123956735	32.0	19.0	35.0	10.0	37.0
5	31.290418946216295	33.0	30.0	35.0	19.0	37.0
6	29.877536094844885	33.0	28.0	35.0	17.0	37.0
7	29.768484225711813	32.0	28.0	35.0	17.0	35.0
8	32.5329686053926	33.0	32.0	35.0	28.0	35.0
9	27.134528712702274	31.0	23.0	34.0	10.0	35.0
10	32.094262480272725	35.0	31.0	37.0	19.0	37.0
11	33.88270409674607	35.0	32.0	38.0	27.0	39.0
12	33.029048254209464	35.0	31.0	37.0	25.0	39.0
13	32.088070250015484	35.0	30.0	37.0	21.0	39.0
14	31.832349549724444	34.0	27.0	38.0	20.0	40.0
15	31.37865990095779	33.0	27.0	38.0	17.0	40.0
16	30.219127966622207	33.0	26.0	37.0	17.0	40.0
17	29.96035633774767	32.0	25.0	37.0	17.0	40.0
18	31.05715261169863	33.0	27.0	37.0	18.0	39.0
19	30.615082599330904	34.0	27.0	37.0	18.0	39.0
20	31.342355022317133	34.0	29.0	35.0	19.0	39.0
21	32.084137347014256	34.0	30.0	36.0	23.0	39.0
22	32.42643881490754	34.0	30.0	36.0	23.0	39.0
23	33.90205565308783	35.0	33.0	37.0	28.0	39.0
24	33.56916135445832	35.0	33.0	37.0	26.0	39.0
25	32.35659882548454	34.0	31.0	36.0	23.0	39.0
26	26.058667197748036	33.0	20.0	35.0	0.0	38.0
27	29.067833372104506	33.0	23.0	35.0	10.0	39.0
28	30.62018700535377	33.0	27.0	36.0	18.0	39.0
29	32.033248929329915	34.0	30.0	36.0	23.0	39.0
30	31.674616709314954	34.0	30.0	36.0	21.0	39.0
31	30.611681893416655	33.0	29.0	35.0	18.0	38.0
32	30.712523199943767	34.0	29.0	36.0	18.0	38.0
33	30.436301196774014	33.0	29.0	35.0	17.0	38.0
34	30.539808509463235	34.0	29.0	36.0	15.0	38.0
35	29.761769839822065	33.0	27.0	36.0	10.0	38.0
36	29.358665691529865	33.0	26.0	35.0	10.0	38.0
37	29.365468776934108	33.0	26.0	36.0	10.0	38.0
38	29.196551429819436	33.0	26.0	35.0	10.0	38.0
39	29.13331202313551	33.0	25.0	35.0	10.0	38.0
40	29.134316168582632	33.0	26.0	35.0	10.0	38.0
41	28.629610910375	33.0	23.0	35.0	10.0	38.0
42	28.748510099192835	33.0	24.0	35.0	9.0	38.0
43	28.323664528394723	32.0	23.0	35.0	8.0	38.0
44	27.98038903941773	32.0	23.0	35.0	8.0	38.0
45	27.369460255086416	31.0	23.0	34.0	8.0	37.0
46	27.051266645802755	31.0	23.0	34.0	8.0	37.0
47	27.301325639347773	31.0	23.0	34.0	8.0	37.0
48	27.470689831186416	31.0	23.0	34.0	8.0	37.0
49	27.35870585734775	31.0	23.0	34.0	8.0	37.0
50	29.012945108389133	33.0	26.0	35.0	10.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	17.0
12	24.0
13	76.0
14	215.0
15	469.0
16	917.0
17	1840.0
18	2974.0
19	4798.0
20	7019.0
21	9838.0
22	13224.0
23	17289.0
24	22062.0
25	27307.0
26	32178.0
27	35204.0
28	39586.0
29	46712.0
30	55536.0
31	63989.0
32	65535.0
33	58226.0
34	42985.0
35	31150.0
36	15129.0
37	3135.0
38	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.20844051191335	23.018528157075124	27.641446438045065	25.131584892966462
2	37.749007151189154	17.5507888399275	31.806307037553367	12.893896971329974
3	28.307027511911677	14.231418706894965	43.669113992264734	13.792439788928625
4	25.796663894109518	23.858997896315287	35.92363808589795	14.420700123677246
5	18.010687454708858	29.6847150653615	39.709099063969084	12.595498415960558
6	18.554766929473846	33.66598440562121	37.751350157232444	10.027898507672509
7	78.08569711960878	3.6609469426281502	16.766718603300625	1.486637334462439
8	81.5136823185049	1.4851311162917578	15.71454153229248	1.286645032910867
9	69.90944281642714	2.190710650468685	15.902986161202163	11.996860371902002
10	56.91496394281057	14.509901710896484	20.808404027962105	7.766730318330843
11	52.055402051469144	14.241794876515213	24.81678529529407	8.886017776721564
12	45.134831629912156	17.810193080433724	28.305353936166473	8.749621353487647
13	18.3417207371097	43.36268227331835	28.657306915382335	9.638290074189612
14	11.326425928374304	44.27461369687861	34.181613092717775	10.217347282029312
15	9.270103410245296	17.255068005750406	60.00388269572887	13.470945888275429
16	10.07777106487951	11.432028557896516	58.711547505284315	19.77865287193966
17	11.544994920697613	12.394167253812824	36.84176173971546	39.2190760857741
18	19.075583701380534	18.161978702075064	44.180056667274734	18.582380929269668
19	28.24041919725266	19.323607626819385	33.4972879705049	18.938685205423056
20	30.968682377080047	17.739902899135263	32.70920115208955	18.582213571695146
21	18.946885726574543	25.638845701337022	35.83728157744556	19.576986994642883
22	19.875552907586822	19.94534101616172	32.42820778447022	27.750898291781233
23	15.653121302443587	26.959464321875476	31.268921865769183	26.11849250991175
24	16.303472837028867	19.008305956423435	50.55721704436482	14.131004162182878
25	13.731354274228774	18.704719316243892	47.88301036110744	19.680916048419896
26	9.909074629763206	43.934543105453685	29.39167195237673	16.764710312406383
27	13.16869810869205	34.68000394963876	36.509724311867494	15.641573629801698
28	11.44792752747593	25.56303272007939	49.48746742803206	13.501572324412617
29	11.862806954711367	19.853796422899205	48.317637982136254	19.965758640253178
30	15.920726064101299	27.967124278061263	39.59630005874251	16.51584959909493
31	30.260425121710792	22.900875782187462	30.793124281408414	16.045574814693325
32	30.79061391779061	21.179435770673262	33.10416502795708	14.925785283579044
33	29.068337118403807	24.1515389365765	29.64220624143338	17.137917703586307
34	17.68818940860854	25.32337667336655	31.330509453192594	25.657924464832316
35	17.995290557853004	23.91991605344062	36.114760435999955	21.970032952706422
36	32.4330611541313	21.32855136957071	28.655298624488097	17.58308885180989
37	18.9000256057089	31.899023133837527	31.314777841187702	17.88617341926587
38	18.995084708036345	32.792377866626055	26.14142049762101	22.071116927716588
39	19.49180198921213	30.0544079474765	30.230802831020732	20.22298723229064
40	23.719589036740008	23.191743246703474	27.807297794394525	25.281369922161996
41	15.447104128209292	21.264286060954976	30.793124281408414	32.49548552942732
42	21.41139336895818	22.030449037108195	25.650393373978908	30.907764219954714
43	21.8992406986844	22.4533616279206	27.891813369527195	27.755584303867803
44	17.768018971654648	28.225859088269406	31.16097623020369	22.845145709872256
45	14.7584277090589	39.75930633632513	24.291617226449862	21.19064872816611
46	21.170231104074656	31.017885503988968	27.619355238208403	20.192528153727974
47	20.075712566712912	25.31785387340738	28.212470482307793	26.393963077571907
48	22.079484806442597	20.94597195421766	34.20872501979003	22.765818219549708
49	20.786480185699965	20.750498307178134	35.18458703681699	23.27843447030491
50	18.10758749035602	32.06135998112207	29.068337118403807	20.762715410118105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	164.0
1	289.0
2	414.0
3	4834.0
4	9254.0
5	7550.0
6	5846.0
7	6193.5
8	6541.0
9	7433.0
10	8325.0
11	8922.0
12	9519.0
13	9550.0
14	9581.0
15	9144.0
16	8707.0
17	8233.0
18	7759.0
19	7236.5
20	6714.0
21	6142.0
22	5570.0
23	5016.5
24	4463.0
25	4242.5
26	4022.0
27	3849.5
28	3677.0
29	4010.5
30	4344.0
31	4972.5
32	5601.0
33	6251.0
34	6901.0
35	8215.0
36	9529.0
37	11127.0
38	12725.0
39	17794.0
40	22863.0
41	35284.0
42	47705.0
43	56354.5
44	65004.0
45	65088.5
46	65173.0
47	61528.0
48	57883.0
49	52417.0
50	46951.0
51	42720.0
52	38489.0
53	34458.5
54	30428.0
55	26634.5
56	22841.0
57	20129.5
58	17418.0
59	15791.0
60	14164.0
61	13168.0
62	12172.0
63	10694.5
64	9217.0
65	7756.5
66	6296.0
67	5224.0
68	4152.0
69	3515.5
70	2879.0
71	2318.0
72	1757.0
73	1392.0
74	1027.0
75	862.5
76	698.0
77	554.0
78	410.0
79	287.5
80	165.0
81	131.5
82	98.0
83	69.0
84	40.0
85	29.0
86	18.0
87	14.5
88	11.0
89	7.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	597523.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.94829425818666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.6686596031386	32.587515744110966
2	6.3935234524867575	4.980336655481434
3	2.414707390848973	2.8214620201861176
4	1.299494669461803	2.024524030925732
5	0.8258568888087292	1.608285856023646
6	0.608102906681966	1.4210742569224697
7	0.4664131201970118	1.2716196815918537
8	0.3821759209939159	1.1908080183411627
9	0.29578480248245614	1.0368282171767689
>10	2.840776160162531	24.78255807941378
>50	0.6608096994492976	17.835031412701507
>100	0.14275002091103964	7.296868884370522
>500	4.7268218844715104E-4	0.10456973700826182
>1k	0.0	0.0
>5k	4.7268218844715104E-4	1.0385174057457833
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5641	0.9440640778681323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.027446642221303615	0.0
2	0.0	0.0	0.0	0.10643941739481158	0.0
3	0.0	0.0	0.0	0.14124979289500153	0.0
4	0.0	0.0	0.0	0.20484567121265626	0.0
5	0.0	0.0	0.0	0.3377275853816506	0.0
6	0.0	0.0	0.0	0.4443343603509823	0.0
7	0.0	0.0	0.0	0.5213188446302486	0.0
8	0.0	0.0	0.0	0.7492598611266846	0.0
9	0.0	0.0	0.0	0.8801334844014372	0.0
10	0.0	0.0	0.0	1.0568630830947092	0.0
11	0.0	0.0	0.0	1.1952677972228685	0.0
12	0.0	0.0	0.0	1.321622765985577	0.0
13	0.0	0.0	0.0	1.3721647534906607	0.0
14	0.0	0.0	0.0	1.387896365495554	0.0
15	0.0	0.0	0.0	1.4118284986519347	0.0
16	0.0	0.0	0.0	1.4662197103709815	0.0
17	0.0	0.0	0.0	1.5522415036743356	0.0
18	0.0	0.0	0.0	1.7005203146991832	0.0
19	0.0	0.0	0.0	1.7381757689662156	0.0
20	0.0	0.0	0.0	1.7912281200891012	0.0
21	0.0	0.0	0.0	1.8412680348706243	0.0
22	0.0	0.0	0.0	1.893483598120909	0.0
23	0.0	0.0	0.0	1.9545691128207616	0.0
24	0.0	0.0	0.0	1.9975800094724387	0.0
25	0.0	0.0	0.0	2.0250266516937425	0.0
26	0.0	0.0	0.0	2.058330809023251	0.0
27	0.0	0.0	0.0	2.094814760268642	0.0
28	0.0	0.0	0.0	2.139833947814561	0.0
29	0.0	0.0	0.0	2.189706505021564	0.0
30	0.0	0.0	0.0	2.2727158619835555	0.0
31	0.0	0.0	0.0	2.340830394813254	0.0
32	0.0	0.0	0.0	2.400744406491466	0.0
33	0.0	0.0	0.0	2.455805048508593	0.0
34	0.0	0.0	0.0	2.5212418601459694	0.0
35	0.0	0.0	0.0	2.607096295874803	0.0
36	0.0	0.0	0.0	2.6681818105746555	0.0
37	0.0	0.0	0.0	2.733618622212032	0.0
38	0.0	0.0	0.0	2.798386003551328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGTC	30	2.5256686E-6	44.000004	9
GCGTTAG	60	0.0	44.000004	1
TTCGCAA	30	2.5256686E-6	44.000004	14
CGTCAGA	30	2.5256686E-6	44.000004	30
ACGCGAG	20	7.853443E-4	44.0	1
CGGAATC	40	8.301868E-9	44.0	9
AGCGTAA	25	4.440266E-5	44.0	1
TTGGCCA	20	7.853443E-4	44.0	5
GTACACT	55	1.8189894E-12	44.0	18
CACGGTA	20	7.853443E-4	44.0	30
ACGTTAG	40	8.301868E-9	44.0	1
CCGGCAT	20	7.853443E-4	44.0	17
GACAATC	20	7.853443E-4	44.0	9
AGAAGTC	20	7.853443E-4	44.0	36
GTCTAAA	40	8.301868E-9	44.0	33
ACTTATG	25	4.440266E-5	44.0	1
ATACTCG	35	1.4445322E-7	44.0	44
ACCTAGC	20	7.853443E-4	44.0	34
ATTCACG	20	7.853443E-4	44.0	1
TCTAACG	25	4.440266E-5	44.0	23
>>END_MODULE