FastQCFastQC Report
Thu 26 May 2016
SRR1546045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546045_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences995782
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152931.5357779112295662No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10270.10313502352924636No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAC207.8559754E-444.027
CGTATGA408.310963E-944.015
CTCGTAG408.310963E-944.01
CGTTGAT2200.044.025
CTAAGCG302.5273748E-644.01
CCGTCGA207.8559754E-444.017
GTACTAA207.8559754E-444.026
CTCGAAT207.8559754E-444.039
GACGCGT207.8559754E-444.010
ACGTAAG254.4424094E-544.01
ATACGGA254.4424094E-544.039
ATACGAG254.4424094E-544.01
TAAGCGA207.8559754E-444.023
TACGTTC207.8559754E-444.031
CGTTTTT103000.043.1242751
TGCGTAG1100.042.01
TACGGGT700.040.8571434
CTAACGG650.040.6153832
TATCGGG603.6379788E-1240.3333323
CGGTCTA1750.040.2285731
Produced by FastQC (version 0.11.5)