##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546045_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	995782
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.899499087149596	31.0	28.0	33.0	16.0	33.0
2	28.71746727697428	31.0	28.0	33.0	16.0	33.0
3	28.152821601515193	31.0	27.0	33.0	16.0	34.0
4	32.307654687471754	35.0	32.0	35.0	26.0	37.0
5	33.628226860899275	35.0	33.0	35.0	28.0	37.0
6	33.407289949004905	35.0	33.0	37.0	28.0	37.0
7	34.4001207091512	35.0	35.0	37.0	31.0	37.0
8	34.7643249225232	35.0	35.0	37.0	32.0	37.0
9	36.49748941033278	39.0	37.0	39.0	32.0	39.0
10	36.218942499462734	38.0	35.0	39.0	31.0	39.0
11	36.295433136971745	38.0	35.0	39.0	32.0	39.0
12	35.07079762437964	37.0	34.0	39.0	29.0	39.0
13	35.21852975852144	37.0	34.0	39.0	27.0	39.0
14	35.93070370824136	38.0	34.0	40.0	28.0	41.0
15	36.52466102018313	38.0	35.0	40.0	31.0	41.0
16	36.05961144105839	38.0	34.0	40.0	30.0	41.0
17	35.68888873267442	37.0	34.0	40.0	29.0	41.0
18	35.33711193815514	37.0	33.0	39.0	29.0	40.0
19	34.78512967697749	37.0	33.0	39.0	27.0	40.0
20	35.28541889690715	36.0	34.0	39.0	29.0	40.0
21	35.514970144067675	36.0	34.0	39.0	30.0	40.0
22	35.87875559108319	37.0	34.0	40.0	31.0	41.0
23	36.22946990405531	37.0	35.0	40.0	31.0	41.0
24	35.9177862222856	36.0	35.0	40.0	31.0	41.0
25	35.56946299491254	36.0	34.0	40.0	30.0	41.0
26	35.68610800355901	36.0	34.0	40.0	30.0	41.0
27	35.96295373887055	37.0	35.0	40.0	31.0	41.0
28	35.88819440399605	37.0	35.0	40.0	31.0	41.0
29	36.025495540188516	37.0	35.0	40.0	31.0	41.0
30	35.65193184853713	36.0	35.0	40.0	30.0	41.0
31	34.97284244945179	36.0	34.0	40.0	29.0	41.0
32	34.94678453717781	36.0	34.0	40.0	27.0	41.0
33	34.69687943746724	36.0	34.0	40.0	25.0	41.0
34	34.43996175869819	36.0	34.0	40.0	23.0	41.0
35	34.20697401640118	36.0	34.0	40.0	22.0	41.0
36	33.81991339469884	36.0	33.0	40.0	20.0	41.0
37	33.741012591109296	36.0	33.0	40.0	20.0	41.0
38	33.58319692462808	36.0	33.0	40.0	18.0	41.0
39	33.392786774615324	36.0	33.0	40.0	18.0	41.0
40	33.19873426111338	36.0	33.0	40.0	18.0	40.0
41	32.90257506160987	35.0	33.0	39.0	17.0	40.0
42	33.08847719681617	35.0	33.0	40.0	18.0	40.0
43	32.84912159488724	35.0	33.0	39.0	17.0	40.0
44	32.702756225760254	35.0	32.0	39.0	15.0	40.0
45	32.64624084387948	35.0	32.0	39.0	17.0	40.0
46	32.459238066163074	35.0	32.0	39.0	15.0	40.0
47	32.420478578644726	35.0	32.0	39.0	15.0	40.0
48	32.444606349582536	35.0	32.0	39.0	17.0	40.0
49	32.43266096394592	35.0	32.0	39.0	15.0	40.0
50	32.5836146867487	35.0	32.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	2.0
12	11.0
13	22.0
14	58.0
15	139.0
16	313.0
17	588.0
18	1106.0
19	1888.0
20	2819.0
21	4370.0
22	6094.0
23	8665.0
24	12287.0
25	18231.0
26	25541.0
27	29172.0
28	28001.0
29	27748.0
30	31147.0
31	38008.0
32	51519.0
33	77875.0
34	123149.0
35	94320.0
36	98658.0
37	135140.0
38	147356.0
39	31552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.770955891952255	22.103331853759155	27.863327515460213	21.262384738828377
2	36.646575254423155	20.406072815134234	29.987085526751837	12.960266403690767
3	28.286311662592816	19.151782217392963	38.71188673826199	13.850019381752233
4	25.652100560162765	25.51512278791944	34.079647955074506	14.753128696843284
5	21.319023641720776	29.162105762104556	35.609601298276125	13.909269297898536
6	19.10066661176844	37.18836050460844	32.73427316420662	10.9766997194165
7	77.39716122605148	4.8735566620003175	15.028891865890326	2.700390246057872
8	78.77125716271232	3.468931954986131	14.71336095651458	3.0464499257869693
9	74.91750202353528	5.138574507271672	16.13746783934636	3.806455629846693
10	44.662084673151355	22.44597713154084	21.410609952780828	11.48132824252698
11	38.63375718781822	20.149490551144726	28.07210815218592	13.144644108851134
12	35.32389619414691	20.23063280918916	30.881156719040913	13.564314277623012
13	20.962218638215997	33.03032189776477	31.062923410947374	14.944536053071857
14	16.37105310198417	33.62673757910868	34.072718727592985	15.929490591314163
15	14.982295321666792	22.00772859923156	47.10749943260674	15.902476646494915
16	16.697128487962225	17.78963668754808	45.71623106262214	19.797003761867558
17	17.2894268022519	18.687423552544633	34.61360016549807	29.409549479705397
18	21.121992564637644	21.27262794467062	38.05632156435846	19.549057926333273
19	25.496142730035288	23.19563920617163	31.016427290310528	20.29179077348255
20	26.817415860097892	21.84524323596932	31.641463693860704	19.695877210072084
21	21.40167225356554	25.636032786292585	33.470578901807826	19.491716058334053
22	21.673418479145035	21.791617040677576	31.970150093092663	24.564814387084724
23	18.915987635848005	26.920249612867075	31.54214476662563	22.62161798465929
24	18.926532112450314	22.068986987111636	41.26254541656708	17.741935483870968
25	18.02723889365343	22.860023579458154	38.248632732867236	20.86410479402118
26	17.09410292614247	28.67957042806558	32.71459014121565	21.511736504576305
27	16.80387876061226	28.685897114026965	34.64352639433129	19.866697731029483
28	16.256469789572417	24.63350412037976	41.06631772817745	18.04370836187037
29	16.646715847444522	22.248042242177505	39.715720910801764	21.389520999576213
30	18.694151932852773	26.998981704830978	35.67296858147667	18.633897780839582
31	26.04676525584917	24.596648664065025	30.470122978724255	18.886463101361542
32	25.597871823350893	24.309738476895546	31.09064032087344	19.001749378880117
33	24.77078316338315	25.116742419525558	30.699791721481205	19.412682695610084
34	18.872403799225133	25.528579548535724	32.25786366895566	23.34115298328349
35	20.142862594423278	24.80191447525663	33.33299858804437	21.72222434227572
36	26.72211387633036	24.304416026801047	29.113199475387187	19.86027062148141
37	20.292995856522815	29.19916206559267	30.418806525926357	20.08903555195816
38	20.586232729653677	29.91116529521522	28.646731915218393	20.85587005991271
39	20.12548931392614	28.242326131623187	29.341060593583734	22.291123960866937
40	23.299778465567762	24.73955142792298	29.046719060999294	22.91395104550996
41	19.035491704007505	22.741925441512297	30.832953397430362	27.389629457049836
42	21.50159372232075	24.417593408999156	27.398265885505058	26.682546983175033
43	21.217796666338618	25.406364043535635	28.264118049934623	25.111721240191127
44	19.808351627163375	27.505116581741785	29.82731160033019	22.859220190764646
45	18.402521837108925	32.06956944391443	26.993357983976413	22.53455073500023
46	21.644396062592016	28.82126810888327	28.885539204364008	20.64879662416071
47	20.858681920339993	26.002779724879545	29.945711009036113	23.192827345744348
48	21.20192973964181	24.468709014623684	32.25695985667546	22.072401389059053
49	20.562532763195158	24.135202283230665	32.54919249393944	22.75307245963474
50	19.364881068346286	28.762319463497033	30.593242296004547	21.27955717215214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	376.0
1	869.0
2	1362.0
3	12628.0
4	23894.0
5	17002.0
6	10110.0
7	10135.0
8	10160.0
9	10656.0
10	11152.0
11	11023.0
12	10894.0
13	10373.5
14	9853.0
15	9269.5
16	8686.0
17	8011.5
18	7337.0
19	6832.0
20	6327.0
21	5995.5
22	5664.0
23	5409.0
24	5154.0
25	5768.0
26	6382.0
27	7198.5
28	8015.0
29	8786.0
30	9557.0
31	11792.5
32	14028.0
33	18385.0
34	22742.0
35	23837.0
36	24932.0
37	26299.0
38	27666.0
39	34243.5
40	40821.0
41	55210.5
42	69600.0
43	78838.5
44	88077.0
45	91163.0
46	94249.0
47	88897.0
48	83545.0
49	80479.5
50	77414.0
51	71511.5
52	65609.0
53	59464.0
54	53319.0
55	48202.0
56	43085.0
57	40462.5
58	37840.0
59	34053.0
60	30266.0
61	27912.5
62	25559.0
63	22896.5
64	20234.0
65	17201.0
66	14168.0
67	12037.5
68	9907.0
69	8575.0
70	7243.0
71	5752.5
72	4262.0
73	3751.0
74	3240.0
75	2263.0
76	1286.0
77	1019.0
78	752.0
79	613.5
80	475.0
81	340.5
82	206.0
83	172.5
84	139.0
85	98.0
86	57.0
87	50.5
88	44.0
89	33.5
90	23.0
91	27.0
92	31.0
93	34.0
94	37.0
95	20.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	995782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.566453732644497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.6271300227499	21.12338234437469
2	7.24652016707597	3.9952172581675
3	3.1898545646365983	2.637989348097589
4	2.006703479786829	2.2127079452272125
5	1.4412370850750975	1.9864897711747047
6	1.1188317865928792	1.8505334807834752
7	0.8787312055356779	1.6956452184581128
8	0.688731123984902	1.5188699730849635
9	0.5517338278872708	1.3688410535270363
>10	4.955425271321019	30.076347902150086
>50	1.020116681816545	19.849529220500038
>100	0.27352015086876513	9.931375764499489
>500	7.323163343206503E-4	0.10578178918200051
>1k	3.6615816716032513E-4	0.10366211592549097
>5k	0.0	0.0
>10k+	3.6615816716032513E-4	1.543626814847647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15293	1.5357779112295662	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1027	0.10313502352924636	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.036052067621226334	0.0
2	0.0	0.0	0.0	0.13356337029590815	0.0
3	0.0	0.0	0.0	0.17734805409216073	0.0
4	0.0	0.0	0.0	0.27345342655320143	0.0
5	0.0	0.0	0.0	0.48494550011950405	0.0
6	0.0	0.0	0.0	0.70216171812706	0.0
7	0.0	0.0	0.0	0.8587220897746696	0.0
8	0.0	0.0	0.0	1.3176578809418125	0.0
9	0.0	0.0	0.0	1.5258359761473896	0.0
10	0.0	0.0	0.0	1.8459863705108146	0.0
11	0.0	0.0	0.0	2.083186882269412	0.0
12	0.0	0.0	0.0	2.310043764599079	0.0
13	0.0	0.0	0.0	2.413279211715014	0.0
14	0.0	0.0	0.0	2.4452139122820054	0.0
15	0.0	0.0	0.0	2.492212150852295	0.0
16	0.0	0.0	0.0	2.5949454800347866	0.0
17	0.0	0.0	0.0	2.7209770813290457	0.0
18	0.0	0.0	0.0	2.92192467829304	0.0
19	0.0	0.0	0.0	3.0042720193777352	0.0
20	1.0042358668865273E-4	0.0	0.0	3.096260024784541	0.0
21	1.0042358668865273E-4	0.0	0.0	3.2161657872907927	0.0
22	1.0042358668865273E-4	0.0	0.0	3.3385821394642603	0.0
23	1.0042358668865273E-4	0.0	0.0	3.467927719119245	0.0
24	1.0042358668865273E-4	0.0	0.0	3.5650373274471723	0.0
25	1.0042358668865273E-4	0.0	0.0	3.651200764826036	0.0
26	1.0042358668865273E-4	0.0	0.0	3.727321843536035	0.0
27	1.0042358668865273E-4	0.0	0.0	3.813786551674965	0.0
28	1.0042358668865273E-4	0.0	0.0	3.9064775221885917	0.0
29	1.0042358668865273E-4	0.0	0.0	3.9984655275953975	0.0
30	1.0042358668865273E-4	0.0	0.0	4.1291166138773345	0.0
31	2.0084717337730547E-4	0.0	0.0	4.237674511087768	0.0
32	3.012707600659582E-4	0.0	0.0	4.341412076137146	0.0
33	3.012707600659582E-4	0.0	0.0	4.452279715841419	0.0
34	3.012707600659582E-4	0.0	0.0	4.560837613051853	0.0
35	3.012707600659582E-4	0.0	0.0	4.702736141042919	0.0
36	3.012707600659582E-4	0.0	0.0	4.807578365545872	0.0
37	3.012707600659582E-4	0.0	0.0	4.9152324504761085	0.0
38	4.0169434675461094E-4	0.0	0.0	5.043573794264207	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAC	20	7.8559754E-4	44.0	27
CGTATGA	40	8.310963E-9	44.0	15
CTCGTAG	40	8.310963E-9	44.0	1
CGTTGAT	220	0.0	44.0	25
CTAAGCG	30	2.5273748E-6	44.0	1
CCGTCGA	20	7.8559754E-4	44.0	17
GTACTAA	20	7.8559754E-4	44.0	26
CTCGAAT	20	7.8559754E-4	44.0	39
GACGCGT	20	7.8559754E-4	44.0	10
ACGTAAG	25	4.4424094E-5	44.0	1
ATACGGA	25	4.4424094E-5	44.0	39
ATACGAG	25	4.4424094E-5	44.0	1
TAAGCGA	20	7.8559754E-4	44.0	23
TACGTTC	20	7.8559754E-4	44.0	31
CGTTTTT	10300	0.0	43.124275	1
TGCGTAG	110	0.0	42.0	1
TACGGGT	70	0.0	40.857143	4
CTAACGG	65	0.0	40.615383	2
TATCGGG	60	3.6379788E-12	40.333332	3
CGGTCTA	175	0.0	40.22857	31
>>END_MODULE