FastQCFastQC Report
Thu 26 May 2016
SRR1546044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546044_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1000827
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157331.571999956036358No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10470.10461348464819593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC207.8559923E-444.0000049
ATTACGC207.8559923E-444.00000435
TCTTACG207.8559923E-444.00000435
CGTAGTG207.8559923E-444.00000430
TGCTACG207.8559923E-444.0000041
CTATACG254.442427E-544.01
CGTTGAT3350.043.99999625
CGTTTTT104600.043.011471
TCGTTGA3600.040.94444724
GGCCTAT3550.040.901418
CGACGGT2100.040.85714328
CGTAAGG2050.040.7804872
CCTATGT3600.040.33333210
ACGTTAG557.8216544E-1140.01
ATTAATC3700.039.83783719
CTCACGA2100.039.80952524
AATCGTT3750.039.30666722
TACGGGA3200.039.1875044
AGGGCGC3050.038.950826
TAGTAGG3900.038.9230772
Produced by FastQC (version 0.11.5)