##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546044_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1000827 Sequences flagged as poor quality 0 Sequence length 50 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.865088571751162 31.0 28.0 33.0 16.0 33.0 2 28.673476035318792 31.0 28.0 33.0 16.0 33.0 3 28.129762686258463 31.0 26.0 33.0 16.0 34.0 4 32.29503300770263 35.0 32.0 35.0 26.0 37.0 5 33.666404883161626 35.0 33.0 35.0 28.0 37.0 6 33.50021732027613 35.0 33.0 37.0 28.0 37.0 7 34.4669818060464 35.0 35.0 37.0 32.0 37.0 8 34.79881038381259 35.0 35.0 37.0 32.0 37.0 9 36.60727977962225 39.0 37.0 39.0 32.0 39.0 10 36.215328922980696 38.0 35.0 39.0 31.0 39.0 11 36.343278109003855 38.0 35.0 39.0 32.0 39.0 12 35.027647135818675 37.0 34.0 39.0 29.0 39.0 13 35.22514180772501 37.0 34.0 39.0 27.0 39.0 14 35.93739977038989 38.0 34.0 40.0 28.0 41.0 15 36.54891804477697 38.0 35.0 40.0 31.0 41.0 16 36.07917252432239 38.0 34.0 40.0 31.0 41.0 17 35.728356649051236 37.0 34.0 40.0 30.0 41.0 18 35.410927163236 37.0 33.0 39.0 29.0 40.0 19 34.8684857622746 37.0 33.0 39.0 27.0 40.0 20 35.26469409798097 36.0 34.0 39.0 29.0 40.0 21 35.41890456592398 36.0 34.0 39.0 30.0 40.0 22 35.84593541141476 37.0 34.0 40.0 31.0 41.0 23 36.24438988956133 37.0 35.0 40.0 32.0 41.0 24 35.78996469919377 36.0 34.0 40.0 31.0 41.0 25 35.484254521510714 36.0 34.0 40.0 30.0 41.0 26 35.66186064124969 36.0 34.0 40.0 30.0 41.0 27 35.98859043570967 37.0 35.0 40.0 31.0 41.0 28 35.9034828197081 37.0 35.0 40.0 31.0 41.0 29 36.054358045896045 37.0 35.0 40.0 31.0 41.0 30 35.698610249323806 37.0 35.0 40.0 30.0 41.0 31 35.00208127878245 36.0 34.0 40.0 29.0 41.0 32 35.016516340986 36.0 34.0 40.0 29.0 41.0 33 34.785205634939906 37.0 34.0 40.0 26.0 41.0 34 34.45691912788124 37.0 34.0 40.0 23.0 41.0 35 34.249794420014645 37.0 34.0 40.0 23.0 41.0 36 33.848953915112205 36.0 33.0 40.0 21.0 41.0 37 33.76566279686699 36.0 33.0 40.0 20.0 41.0 38 33.65469756511365 36.0 33.0 40.0 19.0 41.0 39 33.431175417929374 36.0 33.0 40.0 18.0 41.0 40 33.21551876598053 36.0 33.0 40.0 18.0 40.0 41 32.90286333202442 35.0 33.0 39.0 17.0 40.0 42 33.080492432758106 35.0 33.0 40.0 17.0 40.0 43 32.86332503020002 35.0 33.0 39.0 17.0 40.0 44 32.70535667003388 35.0 32.0 39.0 15.0 40.0 45 32.64194511139288 35.0 32.0 39.0 17.0 40.0 46 32.443637112108284 35.0 32.0 39.0 16.0 40.0 47 32.40918260598485 35.0 32.0 38.0 16.0 40.0 48 32.44277682356691 35.0 32.0 38.0 17.0 40.0 49 32.4931381747295 35.0 32.0 39.0 17.0 40.0 50 32.67346404523459 35.0 32.0 39.0 17.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 4.0 11 5.0 12 10.0 13 27.0 14 49.0 15 113.0 16 288.0 17 539.0 18 1042.0 19 1885.0 20 2913.0 21 4205.0 22 5944.0 23 8447.0 24 12121.0 25 18181.0 26 25597.0 27 29094.0 28 28121.0 29 27721.0 30 30678.0 31 38015.0 32 51308.0 33 78570.0 34 124462.0 35 95583.0 36 100531.0 37 139216.0 38 147946.0 39 28211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.871223498167016 22.096326338118377 27.69619524653112 21.33625491718349 2 36.82634461300505 20.447789677936345 29.784568162129922 12.94129754692869 3 28.099361827768437 19.23539233054264 38.71608180035111 13.949164041337813 4 25.407687842154537 25.677364819294446 34.100398970051764 14.814548368499251 5 21.242632343052296 29.183065604744872 35.658610329257705 13.915691722945125 6 19.020569988619414 37.25888690053326 32.807168471673926 10.913374639173403 7 77.27139655504898 4.8717710453455 15.125191466657077 2.7316409329484515 8 78.62777483021542 3.5234860770143097 14.81095134323914 3.037787749531138 9 74.89556137074639 5.154437280369135 16.231676403614212 3.718324945270261 10 44.651972818479116 22.181056266467632 21.446963361300206 11.720007553753046 11 38.60827095991615 20.179311709216478 28.147222247201565 13.06519508366581 12 35.343371032156405 19.846187203182968 30.94890525535382 13.861536509306804 13 21.08416339687079 32.94235667103306 30.99666575741861 14.976814174677541 14 16.34618170772771 33.6095049394151 34.119483187404015 15.924830165453171 15 15.002692773076664 22.007899467140675 47.10454454166405 15.884863218118618 16 16.737957708974676 17.75881346126753 45.73657585177058 19.766652977987203 17 17.16700288861112 18.60980968738853 34.75605674107513 29.46713068292522 18 21.088859513182598 21.182681922050463 38.11937527664621 19.609083288120726 19 25.534183230468404 23.092002913590463 31.134851477827834 20.2389623781133 20 26.75247570259395 21.56926222014394 31.70507989892359 19.973182178338515 21 21.310076566679356 25.649887543001938 33.58952146574783 19.45051442457088 22 21.658488430068335 21.801769936262712 31.981950926583714 24.55779070708524 23 18.9263479102782 26.93452514770285 31.58847633007503 22.550650611943922 24 18.914857412919517 22.081138898131243 41.25428270820032 17.749720980748922 25 17.954151916365166 22.778662046487554 38.33030084120432 20.936885195942956 26 17.071481884481535 28.752921334056737 32.74372094278032 21.43187583868141 27 16.759539860535337 28.596051065768606 34.648645570113516 19.995763503582538 28 16.135955564747952 24.703969816961376 41.07622995782488 18.083844660465793 29 16.68630042954477 22.200340318556556 39.78130086418532 21.332058387713364 30 18.744897969379323 26.912143657195497 35.721758106046295 18.621200267378878 31 26.049656933715816 24.54400211025482 30.526354704659248 18.879986251370116 32 25.57325092148793 24.317989023077917 31.16872346569387 18.940036589740288 33 24.780406603738708 25.15909342973361 30.675131666112126 19.385368300415557 34 18.80384921669779 25.46184305579286 32.325666673660876 23.408641053848466 35 20.17511517974635 24.648615594903013 33.519279555807344 21.65698966954329 36 26.751076859437244 24.339970844111917 29.039384429077153 19.869567867373682 37 20.327289331722664 29.11582121585449 30.395962538980264 20.160926913442584 38 20.600063747281 29.815042959472514 28.733137695126132 20.851755598120356 39 20.118462031899618 28.167205720868843 29.373707943530704 22.34062430370084 40 23.25147103345533 24.71426130590002 29.099734519552328 22.934533141092317 41 19.03226032071477 22.712616666017205 30.790736061277325 27.464386951990704 42 21.532992215437833 24.422003003516092 27.427017856232894 26.61798692481318 43 21.17588754100359 25.41897850477655 28.31558301284837 25.089550941371485 44 19.778043557977554 27.542022747188078 29.775575598979643 22.90435809585473 45 18.41377181071254 32.031410023910226 27.117174097021763 22.43764406835547 46 21.667980580060288 28.845844486609572 28.819466301368767 20.666708631961367 47 20.83307105024145 26.029273790575196 29.91565974938726 23.221995409796097 48 21.16229877890984 24.42679903719624 32.41998866937043 21.990913514523488 49 20.629739205676906 24.097671225896182 32.64839977338741 22.624189795039502 50 19.304135479958074 28.82576109557396 30.56901942093888 21.30108400352908 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 406.0 1 901.5 2 1397.0 3 12975.0 4 24553.0 5 17337.5 6 10122.0 7 10308.5 8 10495.0 9 10880.5 10 11266.0 11 11169.0 12 11072.0 13 10446.5 14 9821.0 15 9269.5 16 8718.0 17 8027.0 18 7336.0 19 6818.0 20 6300.0 21 5974.0 22 5648.0 23 5410.0 24 5172.0 25 5792.0 26 6412.0 27 7091.5 28 7771.0 29 8752.5 30 9734.0 31 11822.0 32 13910.0 33 18268.5 34 22627.0 35 23597.0 36 24567.0 37 26195.5 38 27824.0 39 34372.5 40 40921.0 41 55606.5 42 70292.0 43 79395.0 44 88498.0 45 91240.5 46 93983.0 47 88885.5 48 83788.0 49 80652.0 50 77516.0 51 71958.5 52 66401.0 53 60121.0 54 53841.0 55 48504.0 56 43167.0 57 40687.0 58 38207.0 59 34338.0 60 30469.0 61 28103.0 62 25737.0 63 23006.0 64 20275.0 65 17354.5 66 14434.0 67 12250.0 68 10066.0 69 8754.0 70 7442.0 71 5930.0 72 4418.0 73 3827.5 74 3237.0 75 2246.0 76 1255.0 77 992.0 78 729.0 79 600.0 80 471.0 81 345.5 82 220.0 83 164.0 84 108.0 85 79.0 86 50.0 87 44.5 88 39.0 89 31.0 90 23.0 91 30.5 92 38.0 93 41.5 94 45.0 95 24.5 96 4.0 97 3.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1000827.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.480192915984375 #Duplication Level Percentage of deduplicated Percentage of total 1 76.33588816596118 20.977249332136218 2 7.377192377434681 4.0545333942046895 3 3.2608080174880567 2.6882290014768104 4 2.0272432654807484 2.2283614409216437 5 1.4395932103514153 1.9780149570499086 6 1.1301962605148477 1.8634806763123295 7 0.8701851710671298 1.6738999461487516 8 0.6828824538160542 1.5012593255844768 9 0.5826929663412214 1.4411263613259557 >10 4.9789948527924555 29.912318082845008 >50 1.0411171191294493 20.08078257313413 >100 0.2717392610207656 9.802309486512756 >500 7.334393010007098E-4 0.10742643506793038 >1k 3.667196505003549E-4 0.10551170498698229 >5k 0.0 0.0 >10k+ 3.667196505003549E-4 1.5854972822924474 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15733 1.571999956036358 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 1047 0.10461348464819593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.03706934365279913 0.0 2 0.0 0.0 0.0 0.12399745410545479 0.0 3 9.99173683363858E-5 0.0 0.0 0.16975960880351948 0.0 4 9.99173683363858E-5 0.0 0.0 0.2611840008313125 0.0 5 9.99173683363858E-5 0.0 0.0 0.46701377960426727 0.0 6 9.99173683363858E-5 0.0 0.0 0.6805371957391237 0.0 7 9.99173683363858E-5 0.0 0.0 0.8349095298188398 0.0 8 9.99173683363858E-5 0.0 0.0 1.2924311594311504 0.0 9 9.99173683363858E-5 0.0 0.0 1.4848720108470295 0.0 10 9.99173683363858E-5 0.0 0.0 1.8152987479354574 0.0 11 9.99173683363858E-5 0.0 0.0 2.052202828261028 0.0 12 9.99173683363858E-5 0.0 0.0 2.2748187249144958 0.0 13 9.99173683363858E-5 0.0 0.0 2.3737369195675178 0.0 14 9.99173683363858E-5 0.0 0.0 2.4024132042800606 0.0 15 9.99173683363858E-5 0.0 0.0 2.4526716405532625 0.0 16 9.99173683363858E-5 0.0 0.0 2.553288430468003 0.0 17 9.99173683363858E-5 0.0 0.0 2.6764865456267666 0.0 18 9.99173683363858E-5 0.0 0.0 2.87572177808952 0.0 19 1.998347366727716E-4 0.0 0.0 2.9529579038135463 0.0 20 2.9975210500915743E-4 0.0 0.0 3.0478794037331127 0.0 21 2.9975210500915743E-4 0.0 0.0 3.1602864431115467 0.0 22 2.9975210500915743E-4 0.0 0.0 3.2881806745821205 0.0 23 2.9975210500915743E-4 0.0 0.0 3.4215703613111956 0.0 24 2.9975210500915743E-4 0.0 0.0 3.5123952491289705 0.0 25 3.996694733455432E-4 0.0 0.0 3.599822946423308 0.0 26 3.996694733455432E-4 0.0 0.0 3.6838534531942084 0.0 27 3.996694733455432E-4 0.0 0.0 3.764786521546681 0.0 28 4.99586841681929E-4 0.0 0.0 3.8511151277893183 0.0 29 4.99586841681929E-4 0.0 0.0 3.9407410071870563 0.0 30 4.99586841681929E-4 0.0 0.0 4.075229784967831 0.0 31 4.99586841681929E-4 0.0 0.0 4.185138890137856 0.0 32 4.99586841681929E-4 0.0 0.0 4.2851561758425785 0.0 33 5.995042100183149E-4 0.0 0.0 4.38657230470401 0.0 34 5.995042100183149E-4 0.0 0.0 4.497780335662407 0.0 35 5.995042100183149E-4 0.0 0.0 4.6442591976435486 0.0 36 6.994215783547007E-4 0.0 0.0 4.752469707551855 0.0 37 7.993389466910864E-4 0.0 0.0 4.858282200620087 0.0 38 7.993389466910864E-4 0.0 0.0 4.983578580513915 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGAC 20 7.8559923E-4 44.000004 9 ATTACGC 20 7.8559923E-4 44.000004 35 TCTTACG 20 7.8559923E-4 44.000004 35 CGTAGTG 20 7.8559923E-4 44.000004 30 TGCTACG 20 7.8559923E-4 44.000004 1 CTATACG 25 4.442427E-5 44.0 1 CGTTGAT 335 0.0 43.999996 25 CGTTTTT 10460 0.0 43.01147 1 TCGTTGA 360 0.0 40.944447 24 GGCCTAT 355 0.0 40.90141 8 CGACGGT 210 0.0 40.857143 28 CGTAAGG 205 0.0 40.780487 2 CCTATGT 360 0.0 40.333332 10 ACGTTAG 55 7.8216544E-11 40.0 1 ATTAATC 370 0.0 39.837837 19 CTCACGA 210 0.0 39.809525 24 AATCGTT 375 0.0 39.306667 22 TACGGGA 320 0.0 39.187504 4 AGGGCGC 305 0.0 38.95082 6 TAGTAGG 390 0.0 38.923077 2 >>END_MODULE