FastQCFastQC Report
Thu 26 May 2016
SRR1546043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546043_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences986940
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157351.5943218432731474No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGT254.4423807E-544.012
CGCATCG454.802132E-1044.021
CGATTAC351.4458055E-744.010
CGTTCAC302.5273512E-644.021
TATTACG302.5273512E-644.01
TACGTAG454.802132E-1044.01
CGTTTTT104800.043.1393131
CGGTCTA1450.042.48275831
GGCCTAT2650.042.3396228
CGTTGAT2450.042.20408225
CCTATGT2650.041.50943410
TCACGAC1600.041.25000425
ACGGTCT1550.041.1612930
TCGATAG750.041.0666661
AACGGGA1300.040.6153874
AAGGGAC5550.040.0360375
TACGGGA2750.040.0000044
GTAATAG557.8216544E-1140.01
ACGACGG1600.039.87500427
ACGTAAG501.3478711E-939.6000021
Produced by FastQC (version 0.11.5)