##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546043_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	986940
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.762597523658986	31.0	28.0	31.0	16.0	33.0
2	28.583573469511823	31.0	28.0	33.0	16.0	33.0
3	28.036114657425983	31.0	26.0	33.0	16.0	34.0
4	32.199894623786655	35.0	32.0	35.0	26.0	37.0
5	33.60835207814051	35.0	33.0	35.0	28.0	37.0
6	33.44549921981073	35.0	33.0	37.0	28.0	37.0
7	34.39449409285265	35.0	35.0	37.0	32.0	37.0
8	34.765632155956794	35.0	35.0	37.0	32.0	37.0
9	36.59401280726285	39.0	37.0	39.0	32.0	39.0
10	36.29705757188887	38.0	35.0	39.0	32.0	39.0
11	36.36355705514013	38.0	35.0	39.0	32.0	39.0
12	35.202852250390094	37.0	34.0	39.0	30.0	39.0
13	35.30789207043995	37.0	34.0	39.0	29.0	39.0
14	36.016124587107626	38.0	34.0	40.0	29.0	41.0
15	36.52524469572619	38.0	35.0	40.0	31.0	41.0
16	36.04561776805074	37.0	34.0	40.0	30.0	41.0
17	35.69353152167305	37.0	34.0	40.0	30.0	41.0
18	35.34737876669301	37.0	33.0	39.0	29.0	40.0
19	34.76472125965104	37.0	33.0	39.0	27.0	40.0
20	35.35849798366669	36.0	34.0	39.0	30.0	40.0
21	35.49542525381482	36.0	34.0	39.0	30.0	40.0
22	35.86260765598719	37.0	34.0	40.0	31.0	41.0
23	36.23573773481671	37.0	35.0	40.0	31.0	41.0
24	35.80673799825724	36.0	34.0	40.0	31.0	41.0
25	35.50723448233935	36.0	34.0	40.0	30.0	41.0
26	35.661454597037306	36.0	34.0	40.0	30.0	41.0
27	35.990234462074696	37.0	35.0	40.0	31.0	41.0
28	35.91191460473787	37.0	35.0	40.0	31.0	41.0
29	36.04791983301923	37.0	35.0	40.0	31.0	41.0
30	35.69735343587249	37.0	35.0	40.0	30.0	41.0
31	34.98423916347498	36.0	34.0	40.0	29.0	41.0
32	35.006956856546495	36.0	34.0	40.0	28.0	41.0
33	34.78806411737289	37.0	34.0	40.0	26.0	41.0
34	34.47310576124182	37.0	34.0	40.0	23.0	41.0
35	34.236888767300954	37.0	34.0	40.0	22.0	41.0
36	33.87790949804446	36.0	33.0	40.0	21.0	41.0
37	33.82136300079032	36.0	33.0	40.0	20.0	41.0
38	33.677040144284355	36.0	33.0	40.0	19.0	41.0
39	33.422279976493	36.0	33.0	40.0	18.0	41.0
40	33.258332826716924	36.0	33.0	40.0	18.0	40.0
41	32.93552495592437	35.0	33.0	39.0	18.0	40.0
42	33.145244898372745	35.0	33.0	40.0	18.0	40.0
43	32.92508460494052	35.0	33.0	39.0	17.0	40.0
44	32.779697853972884	35.0	32.0	39.0	16.0	40.0
45	32.72158388554522	35.0	32.0	39.0	17.0	40.0
46	32.535835005167485	35.0	32.0	39.0	17.0	40.0
47	32.51974790767422	35.0	32.0	39.0	17.0	40.0
48	32.52828540742092	35.0	32.0	39.0	17.0	40.0
49	32.57672705534278	35.0	32.0	39.0	17.0	40.0
50	32.68622307333779	35.0	32.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	3.0
12	10.0
13	22.0
14	52.0
15	105.0
16	275.0
17	541.0
18	1058.0
19	1864.0
20	2821.0
21	4184.0
22	5894.0
23	8268.0
24	12035.0
25	18047.0
26	25119.0
27	28592.0
28	27830.0
29	27640.0
30	30727.0
31	37499.0
32	50511.0
33	77339.0
34	121813.0
35	92884.0
36	97012.0
37	135426.0
38	147454.0
39	31909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.78797089995339	22.06395525563864	27.697327091819158	21.45074675258881
2	36.762822461345166	20.29677589316473	29.942347052505724	12.998054592984376
3	28.224613451678927	19.23480657385454	38.65614930998845	13.884430664478083
4	25.495369526009686	25.62273289156382	33.98788173546517	14.894015846961315
5	21.234826838510955	29.190933592720935	35.64390945751515	13.930330111252964
6	19.033274565829736	37.198208604373114	32.81901635357772	10.949500476219425
7	77.12170952641499	4.976189028715019	15.111050317141872	2.7910511277281294
8	78.55279956228343	3.581474051107463	14.827649097209559	3.0380772893995585
9	74.91752284840011	5.177822360021886	16.19368958599307	3.7109652055849396
10	44.58315601758972	22.47137617281699	21.453685127768658	11.49178268182463
11	38.58177802095366	20.214805357975155	28.145175998540946	13.058240622530246
12	35.27022919326403	20.299815591626643	30.88961841651975	13.54033679858958
13	20.993170810788904	33.079721158327764	31.012016941252764	14.915091089630575
14	16.378300605913225	33.64581433521794	34.06397552029505	15.911909538573774
15	15.042961071595032	21.96506373234442	47.06365128579245	15.928323910268102
16	16.68287839179687	17.73086509818226	45.81028228666383	19.775974223357043
17	17.284232070845242	18.62129410095852	34.677184023344886	29.41728980485136
18	21.083044561979452	21.14890469532089	38.18357752244311	19.58447322025655
19	25.499321134010174	23.137374105822037	31.081018096338177	20.282286663829616
20	26.860194135408435	21.760390702575638	31.661499179281417	19.717915982734514
21	21.374754291040997	25.635398301821795	33.4561371511946	19.53371025594261
22	21.6713275376416	21.800008105862563	32.02697225768537	24.501692098810466
23	19.00044582244108	26.829189211096928	31.59685492532474	22.57351004113725
24	18.85646543862849	22.101951486412545	41.28680568220966	17.754777392749304
25	18.00464060631852	22.849008045068594	38.259266014144735	20.887085334468154
26	17.071351855229295	28.771252558412876	32.742719922183724	21.414675664174112
27	16.759985409447385	28.725454434920056	34.586702332461954	19.92785782317061
28	16.26491985328389	24.614160941901233	41.07838369100452	18.04253551381036
29	16.628265142764505	22.17439763308813	39.81224795833587	21.385089265811498
30	18.667598840861654	27.02555373173648	35.66853101505664	18.63831641234523
31	26.008875919508785	24.454678095932884	30.594970312278356	18.941475672279974
32	25.66103309218392	24.219506758262913	31.21446085881614	18.904999290737027
33	24.8666585608041	25.141751271607188	30.67045615741585	19.32113401017286
34	18.85626279206436	25.463250045595476	32.284232070845235	23.396255091494925
35	20.134354672016535	24.686911058423007	33.489675157557706	21.689059112002756
36	26.80872190812005	24.33400206699495	29.135509757432065	19.721766267452935
37	20.400632257280076	29.082112387784463	30.393336980971487	20.12391837396397
38	20.64451739720753	29.83798407198006	28.729304719638478	20.788193811173933
39	20.103856364115348	28.19705351895759	29.445153707418893	22.253936409508178
40	23.3032403185604	24.757432062739376	28.978053377104988	22.961274241595234
41	19.104302186556428	22.775447342290313	30.8078505278943	27.312399943258963
42	21.48225829331064	24.472510993576105	27.45627900378949	26.588951709323766
43	21.24850548158956	25.411271201896774	28.25602366911869	25.08419964739498
44	19.81366648428476	27.478772772407645	29.855411676495024	22.852149066812572
45	18.41054167426591	32.12505319472309	27.12900480272357	22.335400328287434
46	21.714085962672502	28.8248525746246	28.898818570531137	20.562242892171763
47	20.842604413642167	26.123979167933207	29.967880519585787	23.065535898838835
48	21.226214359535533	24.493788882809493	32.310474800899755	21.969521956755223
49	20.611182037408557	24.18434757938679	32.599448801345574	22.60502158185908
50	19.382535919103493	28.85494558939753	30.613005856485703	21.149512635013274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	389.0
1	887.5
2	1386.0
3	12995.5
4	24605.0
5	17363.5
6	10122.0
7	10147.5
8	10173.0
9	10568.0
10	10963.0
11	10852.5
12	10742.0
13	10291.5
14	9841.0
15	9155.5
16	8470.0
17	7798.0
18	7126.0
19	6549.5
20	5973.0
21	5784.0
22	5595.0
23	5402.5
24	5210.0
25	5769.0
26	6328.0
27	7110.0
28	7892.0
29	8644.5
30	9397.0
31	11565.5
32	13734.0
33	18034.0
34	22334.0
35	23494.5
36	24655.0
37	26203.0
38	27751.0
39	34039.0
40	40327.0
41	55087.0
42	69847.0
43	78475.0
44	87103.0
45	89684.0
46	92265.0
47	87128.5
48	81992.0
49	79505.0
50	77018.0
51	71395.0
52	65772.0
53	59546.5
54	53321.0
55	47944.5
56	42568.0
57	39964.0
58	37360.0
59	33569.5
60	29779.0
61	27693.0
62	25607.0
63	22689.0
64	19771.0
65	16894.5
66	14018.0
67	11937.0
68	9856.0
69	8517.0
70	7178.0
71	5743.5
72	4309.0
73	3760.5
74	3212.0
75	2237.0
76	1262.0
77	991.5
78	721.0
79	579.5
80	438.0
81	328.0
82	218.0
83	172.0
84	126.0
85	82.5
86	39.0
87	41.5
88	44.0
89	37.0
90	30.0
91	29.0
92	28.0
93	35.0
94	42.0
95	22.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	986940.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.952231253410396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.96613650466584	20.474568785018953
2	7.3579666958050405	3.9662723988045885
3	3.280195290296851	2.652257460612851
4	2.062842010230468	2.2239277959592614
5	1.5020401308001126	2.024166647861372
6	1.1446726386893136	1.851088900044352
7	0.8727876087616474	1.6466501426518498
8	0.7009851665458406	1.511449145116312
9	0.6148588725420773	1.491463666886873
>10	5.155892073963563	30.420760121487746
>50	1.0675361404004704	20.273564082327294
>100	0.2725704807577166	9.642050570932374
>500	0.0011372899058041	0.2140566287947533
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.7909663526803326E-4	1.6077236535014048
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15735	1.5943218432731474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0132328206375261E-4	0.0	0.0	0.0331327132348471	0.0
2	1.0132328206375261E-4	0.0	0.0	0.1161164812450605	0.0
3	1.0132328206375261E-4	0.0	0.0	0.16201592801994041	0.0
4	3.039698461912578E-4	0.0	0.0	0.25199100249255274	0.0
5	3.039698461912578E-4	0.0	0.0	0.4555494761586317	0.0
6	3.039698461912578E-4	0.0	0.0	0.6649847001844084	0.0
7	3.039698461912578E-4	0.0	0.0	0.8234543133321175	0.0
8	4.0529312825501044E-4	0.0	0.0	1.257320607129106	0.0
9	4.0529312825501044E-4	0.0	0.0	1.4490242567937262	0.0
10	4.0529312825501044E-4	0.0	0.0	1.7552232151903864	0.0
11	4.0529312825501044E-4	0.0	0.0	1.977729142602387	0.0
12	4.0529312825501044E-4	0.0	0.0	2.1902040650900765	0.0
13	4.0529312825501044E-4	0.0	0.0	2.2831175147425373	0.0
14	4.0529312825501044E-4	0.0	0.0	2.3163515512594484	0.0
15	4.0529312825501044E-4	0.0	0.0	2.3653920197783047	0.0
16	4.0529312825501044E-4	0.0	0.0	2.4658033923034837	0.0
17	4.0529312825501044E-4	0.0	0.0	2.586074128113158	0.0
18	4.0529312825501044E-4	0.0	0.0	2.7790949804446066	0.0
19	4.0529312825501044E-4	0.0	0.0	2.8569112610695684	0.0
20	4.0529312825501044E-4	0.0	0.0	2.944353253490587	0.0
21	4.0529312825501044E-4	0.0	0.0	3.0515532859140375	0.0
22	4.0529312825501044E-4	0.0	0.0	3.1678724137232255	0.0
23	4.0529312825501044E-4	0.0	0.0	3.291284171276876	0.0
24	5.06616410318763E-4	0.0	0.0	3.3873386426733134	0.0
25	5.06616410318763E-4	0.0	0.0	3.470221087401463	0.0
26	5.06616410318763E-4	0.0	0.0	3.5444910531541938	0.0
27	6.079396923825156E-4	0.0	0.0	3.628386730702981	0.0
28	6.079396923825156E-4	0.0	0.0	3.710154619328429	0.0
29	7.092629744462683E-4	0.0	0.0	3.8033720388270815	0.0
30	0.001013232820637526	0.0	0.0	3.9270877662269235	0.0
31	0.001013232820637526	0.0	0.0	4.038644699779115	0.0
32	0.001013232820637526	0.0	0.0	4.140373274971123	0.0
33	0.001013232820637526	0.0	0.0	4.237542302470262	0.0
34	0.001013232820637526	0.0	0.0	4.349605852432772	0.0
35	0.001013232820637526	0.0	0.0	4.487405516039476	0.0
36	0.0011145561027012787	0.0	0.0	4.590451293898312	0.0
37	0.0012158793847650313	0.0	0.0	4.701400287758121	0.0
38	0.0014185259488925365	0.0	0.0	4.828358360184003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGT	25	4.4423807E-5	44.0	12
CGCATCG	45	4.802132E-10	44.0	21
CGATTAC	35	1.4458055E-7	44.0	10
CGTTCAC	30	2.5273512E-6	44.0	21
TATTACG	30	2.5273512E-6	44.0	1
TACGTAG	45	4.802132E-10	44.0	1
CGTTTTT	10480	0.0	43.139313	1
CGGTCTA	145	0.0	42.482758	31
GGCCTAT	265	0.0	42.339622	8
CGTTGAT	245	0.0	42.204082	25
CCTATGT	265	0.0	41.509434	10
TCACGAC	160	0.0	41.250004	25
ACGGTCT	155	0.0	41.16129	30
TCGATAG	75	0.0	41.066666	1
AACGGGA	130	0.0	40.615387	4
AAGGGAC	555	0.0	40.036037	5
TACGGGA	275	0.0	40.000004	4
GTAATAG	55	7.8216544E-11	40.0	1
ACGACGG	160	0.0	39.875004	27
ACGTAAG	50	1.3478711E-9	39.600002	1
>>END_MODULE