FastQCFastQC Report
Thu 26 May 2016
SRR1546042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546042_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences862466
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85670.9933145190650994No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT15970.18516671961561385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA207.8553864E-444.00000421
TATCGAA207.8553864E-444.00000439
CGTTGAT2050.044.025
CCGTACT254.441913E-544.040
TGCACGA254.441913E-544.032
CGTTTTT100250.043.473321
AGGCGAT850.041.4117666
CCAATCA2400.040.33333641
CGTTAGG1750.040.228572
TTAATCG2300.040.17391620
TGCGTAG1150.040.1739161
CGTTTGG1700.040.117652
ACGTTAG557.8216544E-1140.01
TACGGGT557.8216544E-1140.04
TAGGCGA1100.040.05
CATACGA3650.039.7808218
TCACGAC1550.039.74193625
CACGACG1500.039.626
GCATATC2500.039.628
ACGGGAT2450.039.5102045
Produced by FastQC (version 0.11.5)