##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546042_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	862466
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.585366843446582	28.0	19.0	30.0	16.0	33.0
2	25.882204052101763	30.0	25.0	31.0	10.0	33.0
3	26.201545336279924	30.0	25.0	31.0	16.0	33.0
4	28.35886632052742	32.0	19.0	35.0	10.0	37.0
5	31.111009593421652	33.0	30.0	35.0	19.0	37.0
6	29.60369800084873	33.0	28.0	35.0	17.0	37.0
7	29.545767601273557	32.0	28.0	35.0	17.0	35.0
8	32.29789464164384	33.0	32.0	35.0	28.0	35.0
9	26.84129925121686	30.0	23.0	34.0	10.0	35.0
10	31.248810967620752	34.0	28.0	37.0	17.0	37.0
11	33.51981179547948	35.0	32.0	37.0	27.0	39.0
12	32.99126342371757	35.0	31.0	37.0	23.0	39.0
13	33.04849002743297	35.0	31.0	37.0	23.0	39.0
14	32.73059923521623	35.0	30.0	38.0	21.0	40.0
15	31.69130261366825	34.0	27.0	38.0	18.0	40.0
16	30.19691674802253	33.0	26.0	37.0	17.0	40.0
17	30.330549841964785	32.0	25.0	37.0	17.0	40.0
18	31.259885027351803	34.0	27.0	37.0	17.0	39.0
19	30.8667935895444	34.0	27.0	37.0	18.0	39.0
20	31.63653639679709	34.0	29.0	37.0	19.0	39.0
21	32.57231009686179	34.0	31.0	37.0	23.0	39.0
22	33.036895367469555	34.0	31.0	37.0	25.0	39.0
23	34.36510888545172	35.0	33.0	38.0	28.0	40.0
24	34.0094739966561	35.0	33.0	38.0	27.0	40.0
25	32.792127457778044	35.0	31.0	38.0	23.0	40.0
26	26.541991220523474	33.0	21.0	36.0	0.0	39.0
27	29.81281928794874	34.0	24.0	37.0	15.0	39.0
28	31.29041492650145	34.0	28.0	37.0	19.0	39.0
29	32.57967618433654	35.0	30.0	37.0	23.0	39.0
30	32.26670500634228	35.0	30.0	38.0	22.0	39.0
31	31.14370769398446	34.0	29.0	36.0	19.0	39.0
32	31.52035558503176	34.0	30.0	37.0	19.0	39.0
33	31.299158459579857	34.0	30.0	37.0	18.0	39.0
34	31.37664093425132	34.0	30.0	37.0	17.0	39.0
35	30.618269010024743	34.0	29.0	37.0	13.0	39.0
36	30.399524155155103	34.0	28.0	37.0	12.0	39.0
37	30.486820350019595	34.0	28.0	37.0	11.0	39.0
38	30.22048173493216	34.0	28.0	37.0	10.0	39.0
39	30.082713985246954	34.0	27.0	37.0	10.0	39.0
40	29.964944705066635	34.0	27.0	36.0	10.0	39.0
41	29.368321765727575	33.0	26.0	36.0	10.0	38.0
42	29.578289463004918	33.0	26.0	36.0	10.0	38.0
43	29.101469507203763	33.0	26.0	36.0	10.0	38.0
44	28.892638086602833	33.0	25.0	35.0	10.0	38.0
45	28.3709120127634	32.0	24.0	35.0	10.0	38.0
46	27.91619495725049	31.0	23.0	35.0	8.0	38.0
47	28.024743004361913	31.0	23.0	35.0	9.0	38.0
48	28.103766409342512	32.0	23.0	35.0	9.0	38.0
49	27.936899541547145	31.0	24.0	35.0	9.0	38.0
50	29.828001335704826	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	27.0
12	62.0
13	145.0
14	345.0
15	687.0
16	1275.0
17	2445.0
18	4200.0
19	6289.0
20	9093.0
21	12781.0
22	16610.0
23	21752.0
24	27778.0
25	35501.0
26	41333.0
27	46160.0
28	52050.0
29	61478.0
30	73573.0
31	86546.0
32	94071.0
33	93260.0
34	80540.0
35	59898.0
36	28721.0
37	5682.0
38	156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.85416004804827	22.83916119591961	27.523287874536507	22.78339088149562
2	36.670662959467386	17.599650305055505	31.59753543907818	14.13215129639893
3	29.424348322136755	14.756407788828776	40.68125584080995	15.13798804822451
4	26.735778569821882	25.36714490774129	33.06599912344371	14.83107739899312
5	20.445559593073813	29.272806116415023	36.6094431548606	13.672191135650566
6	19.030315397940324	35.548183928409934	34.0939816757994	11.32751899785035
7	79.63977710425686	3.361871656389933	15.120480111679765	1.8778711276734388
8	81.68426349560445	2.040080420561506	14.406712844332414	1.868943239501615
9	69.02382238836081	3.2458091101562268	14.60532936950558	13.125039131977376
10	45.66545231927983	22.21791931508025	21.586126293674184	10.530502071965737
11	39.829975906296596	19.596134804154598	28.430685963272754	12.14320332627605
12	36.048493505830955	19.99209244190496	31.34767051686675	12.611743535397338
13	20.49843124250695	33.87437881609246	31.266739790322166	14.36045015107842
14	15.687690877089647	33.76701226483131	34.840793724042456	15.704503134036587
15	13.47450218327447	20.249957679491136	47.49903184589306	18.77650829134134
16	14.848005602539693	15.78033221019727	46.04285850108874	23.328803686174297
17	16.375949892517504	17.640579454726332	35.49171793438814	30.491752718368026
18	20.507822917077313	20.62376951671138	39.46393249125183	19.404475074959475
19	26.14723363007933	22.233224266231943	31.856676089260333	19.762866014428397
20	27.395166881940852	21.129992370713744	32.37797200121512	19.096868746130284
21	21.054858974150864	25.35381104878337	34.172013737353126	19.41931623971264
22	21.41209044762344	21.305071736161192	32.29449044947859	24.988347366736775
23	18.39527587174451	26.702385949127272	32.441394791214954	22.46094338791326
24	17.967201025895513	21.369538045557736	43.52415051723778	17.139110411308966
25	17.408338415659284	21.513311829103987	40.20726614150587	20.87108361373086
26	13.463719149508503	40.53864152326005	27.444676079984603	18.552963247246847
27	15.719924031787919	29.59977552738311	35.39420684409588	19.28609359673309
28	15.556439326303877	24.257188109444314	42.710553227605494	17.475819336646314
29	15.886539295462082	21.76016213972493	41.11814262823114	21.235155936581847
30	18.068074567577156	27.097995747078723	36.47239427409312	18.361535411250994
31	26.773809054501857	24.159097286153887	30.99948287816563	18.06761078117862
32	26.055172029969874	23.32347014259113	31.885894632368117	18.735463195070878
33	25.14997692662667	24.720394774982434	31.069050837946076	19.060577460444815
34	18.399449949331338	24.775701303007885	32.65172192295117	24.173126824709612
35	20.006353873659947	24.011265371620446	33.87194393750014	22.110436817219462
36	27.18147729881526	23.714789916356125	28.983055563929476	20.12067722089914
37	19.579206600608025	29.562092882502032	30.295802964986446	20.562897551903493
38	19.927162346109874	30.30565842595534	28.790584208536917	20.976595019397866
39	19.587554755781678	28.321580213017093	29.55409256712728	22.536772464073945
40	23.321383103797714	23.99746772626399	29.280110752191966	23.40103841774632
41	18.374985216808547	22.08075448771314	31.09455908986557	28.449701205612744
42	20.747832378319842	23.67084615509481	27.105068489656404	28.476252976928944
43	20.819951163292234	24.619405286701156	28.381988391426443	26.178655158580167
44	18.97686401550902	27.067617737974597	30.196088889301144	23.759429357215243
45	17.798382776828305	33.06820210883675	26.497276414374593	22.636138699960345
46	20.85206837139087	28.76333675762291	28.57457569341864	21.81001917756758
47	20.231985956547852	25.5308615064246	29.6034858185714	24.633666718456148
48	20.470720005194405	23.752704454436465	33.300559094503434	22.476016445865692
49	20.22479726737054	23.857752073704937	32.59305294353633	23.3243977153882
50	18.802596276259006	29.38249159966886	30.483288616594738	21.331623507477396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	538.5
2	839.0
3	7800.5
4	14762.0
5	11805.5
6	8849.0
7	8887.0
8	8925.0
9	9402.5
10	9880.0
11	10266.5
12	10653.0
13	10605.5
14	10558.0
15	10032.0
16	9506.0
17	9080.5
18	8655.0
19	8059.5
20	7464.0
21	7081.0
22	6698.0
23	6370.0
24	6042.0
25	6376.0
26	6710.0
27	7084.0
28	7458.0
29	8349.0
30	9240.0
31	11034.5
32	12829.0
33	16181.5
34	19534.0
35	20260.0
36	20986.0
37	22079.5
38	23173.0
39	28146.0
40	33119.0
41	45193.0
42	57267.0
43	65063.5
44	72860.0
45	75261.5
46	77663.0
47	74242.0
48	70821.0
49	68158.5
50	65496.0
51	61073.5
52	56651.0
53	51950.0
54	47249.0
55	42821.5
56	38394.0
57	35782.5
58	33171.0
59	30123.5
60	27076.0
61	24933.5
62	22791.0
63	20277.0
64	17763.0
65	15345.5
66	12928.0
67	11196.5
68	9465.0
69	8058.5
70	6652.0
71	5425.5
72	4199.0
73	3538.5
74	2878.0
75	2119.0
76	1360.0
77	1039.5
78	719.0
79	565.0
80	411.0
81	312.5
82	214.0
83	171.0
84	128.0
85	96.5
86	65.0
87	57.0
88	49.0
89	35.5
90	22.0
91	24.5
92	27.0
93	24.5
94	22.0
95	13.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	862466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.61365320474116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85665003372176	34.88210897038883
2	7.0883433235305064	6.041204083701428
3	2.760557111563395	3.5291227021213345
4	1.5982533180202854	2.7242965050977332
5	1.0515039311254888	2.2404211932201807
6	0.7408808044053253	1.8942982598986924
7	0.5616781374303598	1.675461015279961
8	0.44221732690978555	1.5075596648049003
9	0.3516896056633109	1.3488101002303658
>10	3.087409059636702	27.987517424553516
>50	0.4082889635725964	11.828433514346488
>100	0.05193147096121799	3.0480753451309206
>500	0.0	0.0
>1k	2.984567296619449E-4	0.20311175524373185
>5k	2.984567296619449E-4	1.0895794659818727
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8567	0.9933145190650994	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTT	1597	0.18516671961561385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028986649908518132	0.0
2	2.3189319926814506E-4	0.0	0.0	0.1013373280801794	0.0
3	2.3189319926814506E-4	0.0	0.0	0.1376286137656441	0.0
4	2.3189319926814506E-4	0.0	0.0	0.203022495959261	0.0
5	2.3189319926814506E-4	0.0	0.0	0.3440135611142932	0.0
6	2.3189319926814506E-4	0.0	0.0	0.47213455370994334	0.0
7	2.3189319926814506E-4	0.0	0.0	0.5711529497974412	0.0
8	2.3189319926814506E-4	0.0	0.0	0.8517437209118968	0.0
9	2.3189319926814506E-4	0.0	0.0	1.0080397372186265	0.0
10	2.3189319926814506E-4	0.0	0.0	1.2260193445306828	0.0
11	2.3189319926814506E-4	0.0	0.0	1.3968086857916717	0.0
12	2.3189319926814506E-4	0.0	0.0	1.5514814497035245	0.0
13	2.3189319926814506E-4	0.0	0.0	1.6232523948770154	0.0
14	2.3189319926814506E-4	0.0	0.0	1.6476011808001707	0.0
15	2.3189319926814506E-4	0.0	0.0	1.689689796467339	0.0
16	2.3189319926814506E-4	0.0	0.0	1.7546198922624197	0.0
17	2.3189319926814506E-4	0.0	0.0	1.8563050601415012	0.0
18	2.3189319926814506E-4	0.0	0.0	1.9767735771613026	0.0
19	2.3189319926814506E-4	0.0	0.0	2.0201376054244458	0.0
20	2.3189319926814506E-4	0.0	0.0	2.0793863178374568	0.0
21	2.3189319926814506E-4	0.0	0.0	2.1446642534314395	0.0
22	2.3189319926814506E-4	0.0	0.0	2.2164351986049304	0.0
23	2.3189319926814506E-4	0.0	0.0	2.2902931825718347	0.0
24	2.3189319926814506E-4	0.0	0.0	2.348382428988505	0.0
25	2.3189319926814506E-4	0.0	0.0	2.3975437872333516	0.0
26	2.3189319926814506E-4	0.0	0.0	2.4404440290979585	0.0
27	2.3189319926814506E-4	0.0	0.0	2.5012000473062126	0.0
28	2.3189319926814506E-4	0.0	0.0	2.56404310430788	0.0
29	2.3189319926814506E-4	0.0	0.0	2.6352343164832006	0.0
30	2.3189319926814506E-4	0.0	0.0	2.7374992173604524	0.0
31	2.3189319926814506E-4	0.0	0.0	2.819937249700278	0.0
32	2.3189319926814506E-4	0.0	0.0	2.89970851024852	0.0
33	2.3189319926814506E-4	0.0	0.0	2.9740302806139605	0.0
34	2.3189319926814506E-4	0.0	0.0	3.0570480459519564	0.0
35	2.3189319926814506E-4	0.0	0.0	3.1670813690046913	0.0
36	2.3189319926814506E-4	0.0	0.0	3.2495194013445166	0.0
37	2.3189319926814506E-4	0.0	0.0	3.329522555092027	0.0
38	2.3189319926814506E-4	0.0	0.0	3.432135295768181	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAA	20	7.8553864E-4	44.000004	21
TATCGAA	20	7.8553864E-4	44.000004	39
CGTTGAT	205	0.0	44.0	25
CCGTACT	25	4.441913E-5	44.0	40
TGCACGA	25	4.441913E-5	44.0	32
CGTTTTT	10025	0.0	43.47332	1
AGGCGAT	85	0.0	41.411766	6
CCAATCA	240	0.0	40.333336	41
CGTTAGG	175	0.0	40.22857	2
TTAATCG	230	0.0	40.173916	20
TGCGTAG	115	0.0	40.173916	1
CGTTTGG	170	0.0	40.11765	2
ACGTTAG	55	7.8216544E-11	40.0	1
TACGGGT	55	7.8216544E-11	40.0	4
TAGGCGA	110	0.0	40.0	5
CATACGA	365	0.0	39.78082	18
TCACGAC	155	0.0	39.741936	25
CACGACG	150	0.0	39.6	26
GCATATC	250	0.0	39.6	28
ACGGGAT	245	0.0	39.510204	5
>>END_MODULE