FastQCFastQC Report
Thu 26 May 2016
SRR1546041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546041_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1147585
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181581.582279308286532No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGTA207.856475E-444.00000442
ACGTATA207.856475E-444.00000428
ATATGCG302.5277131E-644.01
ACTACGG351.4460784E-744.02
CGTTTTT119650.043.3012961
ACGTTAG800.041.2500041
CTACGGG1300.040.6153873
GTTACGG1250.040.482
ATTTCGT557.8216544E-1140.038
ATAACGC501.3478711E-939.60000211
CGGGATA1950.039.487186
TCGTTGA1400.039.28571324
GTTTTTT137550.039.217742
TTGTACG452.3508619E-839.111111
TATAGCG452.3508619E-839.111111
ACGGTCT1700.038.82353230
TAACGGA404.124522E-738.50000429
ATGGGAC4750.038.442115
CGTTGAT1450.037.93103825
AACTTAA18000.037.8888944
Produced by FastQC (version 0.11.5)