FastQCFastQC Report
Thu 26 May 2016
SRR1546040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546040_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1154104
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184911.602195296091167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT800.044.0000044
CGTTACA207.856493E-444.00000434
ACGTCAT207.856493E-444.00000415
ATATGCG351.4460966E-744.01
ATGTACG254.4428518E-544.01
GTTACGG1150.044.02
GTCGTAC351.4460966E-744.09
CCGCAAT551.8189894E-1244.033
AAATGCG551.8189894E-1244.01
CGTTTTT123050.043.2133261
ATTTCGT557.8216544E-1140.038
CTCACGA1600.039.87500424
TCGTAAG501.3478711E-939.61
TGTAACG501.3478711E-939.61
TTGTACG452.3510438E-839.1111071
GTTTTTT142200.038.5850942
TATCGCG404.1245585E-738.5000041
TACGGGA2200.038.04
CGGGATC1100.038.06
ACGTTAG700.037.7142871
Produced by FastQC (version 0.11.5)