##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546040_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1154104
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.86538388221512	31.0	28.0	33.0	16.0	33.0
2	28.657402625759897	31.0	28.0	33.0	16.0	33.0
3	28.15385528513895	31.0	27.0	33.0	16.0	34.0
4	32.2839917373131	35.0	32.0	35.0	26.0	37.0
5	33.66900816564192	35.0	33.0	35.0	28.0	37.0
6	33.5080945911287	35.0	33.0	37.0	28.0	37.0
7	34.46051222420163	35.0	35.0	37.0	32.0	37.0
8	34.815872746303626	35.0	35.0	37.0	32.0	37.0
9	36.61696952787617	39.0	37.0	39.0	32.0	39.0
10	36.24292351469192	38.0	35.0	39.0	31.0	39.0
11	36.33365104011423	38.0	35.0	39.0	32.0	39.0
12	34.93475458017648	37.0	33.0	39.0	28.0	39.0
13	35.144178514241354	37.0	34.0	39.0	27.0	39.0
14	35.79636323936145	38.0	34.0	40.0	27.0	41.0
15	36.443427108822085	38.0	35.0	40.0	31.0	41.0
16	36.11363360667669	38.0	34.0	40.0	31.0	41.0
17	35.663950562514295	37.0	34.0	40.0	30.0	41.0
18	35.32950496662346	37.0	33.0	39.0	29.0	40.0
19	34.81546463750234	37.0	33.0	39.0	27.0	40.0
20	35.207321870472676	35.0	34.0	39.0	29.0	40.0
21	35.33592553184115	36.0	34.0	39.0	30.0	40.0
22	35.729400469974976	36.0	34.0	39.0	31.0	40.0
23	36.12688197944033	37.0	35.0	40.0	31.0	41.0
24	35.69774214455543	36.0	34.0	40.0	31.0	41.0
25	35.36617583857261	35.0	34.0	40.0	30.0	41.0
26	35.53830417362733	36.0	34.0	40.0	30.0	41.0
27	35.86417081996077	36.0	35.0	40.0	31.0	41.0
28	35.78315212493848	36.0	35.0	40.0	31.0	41.0
29	35.936821118374084	37.0	35.0	40.0	31.0	41.0
30	35.60080633980993	36.0	35.0	40.0	30.0	41.0
31	34.878305594643116	36.0	34.0	40.0	29.0	41.0
32	34.8981521595974	36.0	34.0	40.0	28.0	41.0
33	34.6788876912306	36.0	34.0	40.0	26.0	41.0
34	34.33359385289367	36.0	34.0	40.0	23.0	41.0
35	34.1326059003348	36.0	34.0	40.0	22.0	41.0
36	33.75534613864955	36.0	33.0	40.0	20.0	41.0
37	33.66206078481662	36.0	33.0	40.0	20.0	41.0
38	33.534754233587265	36.0	33.0	40.0	18.0	41.0
39	33.3285059232097	35.0	33.0	40.0	18.0	40.0
40	33.136851618225045	35.0	33.0	39.0	18.0	40.0
41	32.87730655122935	35.0	32.0	39.0	18.0	40.0
42	33.05247534017732	35.0	33.0	39.0	18.0	40.0
43	32.8591166827253	35.0	33.0	39.0	18.0	40.0
44	32.70695968474245	35.0	32.0	39.0	17.0	40.0
45	32.65014937995189	35.0	32.0	39.0	17.0	40.0
46	32.45479870098362	35.0	32.0	39.0	17.0	40.0
47	32.44647016213443	35.0	32.0	38.0	17.0	40.0
48	32.45448070537837	35.0	32.0	38.0	17.0	40.0
49	32.50114114499213	35.0	32.0	38.0	17.0	40.0
50	32.650634604853636	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	7.0
12	6.0
13	21.0
14	58.0
15	123.0
16	299.0
17	680.0
18	1232.0
19	2081.0
20	3242.0
21	4730.0
22	6765.0
23	9760.0
24	13971.0
25	20762.0
26	29523.0
27	33226.0
28	32632.0
29	32696.0
30	37225.0
31	45681.0
32	61105.0
33	94154.0
34	149353.0
35	108750.0
36	113472.0
37	157479.0
38	163974.0
39	31094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.78440764437174	22.672653417716255	27.0776290524944	21.465309885417604
2	37.090938078370755	20.48030333488143	29.541445138393073	12.887313448354739
3	27.945488448181443	18.99092282844527	39.67051496225643	13.39307376111685
4	25.663631700436007	25.51017932526011	34.653202830940714	14.172986143363165
5	21.2391604222843	29.348394945342882	35.93575622300936	13.476688409363454
6	19.40596341404241	36.63214060431296	33.456603564323494	10.505292417321142
7	76.85745825332899	5.310180018438547	15.133211564988944	2.6991501632435204
8	78.65287703707811	3.3655545774037696	14.84874846634272	3.132819919175395
9	75.07252379334965	4.80104046082502	16.338822151209943	3.7876135946153897
10	46.328667087194916	20.93910080893923	21.43073761116849	11.301494492697366
11	39.55258798167236	20.17357187913741	27.154138621822643	13.119701517367584
12	35.936016164921014	19.912676847147225	30.144597020719104	14.006709967212661
13	21.069938237801793	33.06045209097274	31.041829852422314	14.827779818803158
14	15.702917588016332	34.3162314661417	34.66628657382697	15.314564372014999
15	15.121600826268688	21.35284168497813	48.589815129312434	14.935742359440743
16	18.165693906268412	17.89128189487256	46.70818227820023	17.2348419206588
17	17.952974775236893	18.461334507115478	34.206882568641994	29.378808149005636
18	21.912756562666797	20.772997927396492	38.13122560878396	19.183019901152758
19	26.19798562347934	22.518767806020946	32.19068645460028	19.092560115899435
20	27.765781939929155	22.101561037826748	31.096157711956636	19.03649931028746
21	22.316879587974743	24.97521887108961	33.81627652273972	18.891625018195935
22	21.789630743849774	22.20285173606538	32.48615376083958	23.52136375924527
23	19.274866043268197	26.011520625524216	32.36008193369055	22.353531397517035
24	20.213083049707826	21.66017967184933	40.919449200418676	17.207288078024163
25	18.695628816813738	21.838413175935617	38.557790285797466	20.90816772145318
26	16.920138912957587	28.363821631326118	33.823641543569735	20.892397912146563
27	17.68575449006329	28.210369256150226	34.684569154946175	19.419307098840314
28	15.895794486458758	25.09617850730957	40.607778848353355	18.40024815787832
29	17.185452957445776	22.818307535542722	39.61419421473281	20.382045292278686
30	19.804627659205757	25.520490354422133	36.05316331977014	18.621718666601968
31	25.75894373470675	23.34200384020851	31.697923237420543	19.201129187664197
32	27.33410507198658	23.25414347407166	31.622366788435013	17.78938466550675
33	25.981454011076995	24.05797051218954	30.025543625184557	19.935031851548906
34	19.72898456291634	25.54033258701122	32.893915972910584	21.83676687716185
35	20.33178985602684	24.815874479249704	33.81740293769019	21.034932727033265
36	26.76777829381061	24.752795242023247	29.371876364695037	19.107550099471105
37	20.750556275690926	29.528014806291285	30.480182028655996	19.24124688936179
38	21.278931534766365	30.180122415310922	27.7818983384513	20.75904771147141
39	21.294528049465214	28.271195663475734	28.979797314626758	21.454478972432295
40	23.44494083722091	24.700460270478224	28.64325918634716	23.21133970595371
41	18.409866008609278	23.89481363897881	30.04538585777365	27.649934494638266
42	21.91908181585022	24.66034256878063	27.405502450385754	26.0150731649834
43	21.430131080041313	24.82471250424572	29.02702009524272	24.71813632047025
44	20.11300541372355	27.215224971059797	30.059162779090965	22.612606836125686
45	18.623278318071854	31.951713190492363	26.918457955262266	22.506550536173517
46	21.546238467243853	28.364948046276595	28.95233011929601	21.136483367183548
47	21.08267539147252	26.26297110139121	29.703908833172747	22.950444673963524
48	22.174084831176394	23.644489578062288	32.06556774779396	22.11585784296736
49	21.534974317739128	23.211946237080888	32.92606212265099	22.32701732252899
50	20.24107012886187	28.662408240505187	29.96471721785905	21.131804412773892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	387.0
1	1049.5
2	1712.0
3	16195.0
4	30678.0
5	21413.5
6	12149.0
7	12073.0
8	11997.0
9	12519.0
10	13041.0
11	12856.5
12	12672.0
13	12052.0
14	11432.0
15	10650.0
16	9868.0
17	9256.0
18	8644.0
19	8102.0
20	7560.0
21	7065.5
22	6571.0
23	6271.0
24	5971.0
25	6428.5
26	6886.0
27	7821.5
28	8757.0
29	10296.5
30	11836.0
31	14082.0
32	16328.0
33	18519.0
34	20710.0
35	23578.5
36	26447.0
37	29754.0
38	33061.0
39	39593.0
40	46125.0
41	61956.0
42	77787.0
43	88941.5
44	100096.0
45	102861.5
46	105627.0
47	100840.0
48	96053.0
49	92815.0
50	89577.0
51	83393.0
52	77209.0
53	71197.0
54	65185.0
55	58444.0
56	51703.0
57	48310.5
58	44918.0
59	42399.5
60	39881.0
61	36487.5
62	33094.0
63	28783.0
64	24472.0
65	19978.0
66	15484.0
67	13054.5
68	10625.0
69	8676.5
70	6728.0
71	6257.5
72	5787.0
73	4438.0
74	3089.0
75	2449.5
76	1810.0
77	1365.5
78	921.0
79	729.0
80	537.0
81	462.0
82	387.0
83	272.0
84	157.0
85	109.0
86	61.0
87	50.5
88	40.0
89	30.5
90	21.0
91	14.5
92	8.0
93	9.0
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1154104.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.150019019915828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.41848242515223	20.747632513159868
2	7.245324310961659	3.9342138569613523
3	3.1647503849781335	2.577690994363268
4	1.958414498088001	2.1268396348787255
5	1.4055174266232142	1.9079912432821706
6	1.0924015317756819	1.779523341905697
7	0.8849254420068379	1.681801980718515
8	0.7074282919709054	1.5365353265789319
9	0.5722025730044299	1.3981779666283543
>10	5.199860216568518	30.374765438330837
>50	1.072014311631894	20.43936741665644
>100	0.2780364706780154	9.802844021174412
>500	3.210582802273595E-4	0.08080416510403053
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.210582802273595E-4	1.6118121002574204
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18491	1.602195296091167	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.66473038824924E-5	0.0	0.0	0.0444500668917186	0.0
2	8.66473038824924E-5	0.0	0.0	0.15544526316519136	0.0
3	8.66473038824924E-5	0.0	0.0	0.21029300652280902	0.0
4	8.66473038824924E-5	0.0	0.0	0.30907093294885035	0.0
5	8.66473038824924E-5	0.0	0.0	0.5475243132334694	0.0
6	8.66473038824924E-5	0.0	0.0	0.7828583905783187	0.0
7	8.66473038824924E-5	0.0	0.0	0.9524271642763564	0.0
8	8.66473038824924E-5	0.0	0.0	1.3868767459431732	0.0
9	8.66473038824924E-5	0.0	0.0	1.5528929801820286	0.0
10	8.66473038824924E-5	0.0	0.0	1.8186402611896328	0.0
11	8.66473038824924E-5	0.0	0.0	2.056573757650957	0.0
12	8.66473038824924E-5	0.0	0.0	2.2562957931001018	0.0
13	8.66473038824924E-5	0.0	0.0	2.33722437492635	0.0
14	8.66473038824924E-5	0.0	0.0	2.36746428398134	0.0
15	8.66473038824924E-5	0.0	0.0	2.416073421459418	0.0
16	8.66473038824924E-5	0.0	0.0	2.5176240616096988	0.0
17	8.66473038824924E-5	0.0	0.0	2.6392768762607184	0.0
18	8.66473038824924E-5	0.0	0.0	2.8402986212681007	0.0
19	8.66473038824924E-5	0.0	0.0	2.925559568288473	0.0
20	8.66473038824924E-5	0.0	0.0	3.02035171873592	0.0
21	1.732946077649848E-4	0.0	0.0	3.1443440105917664	0.0
22	2.5994191164747715E-4	0.0	0.0	3.267469829408788	0.0
23	2.5994191164747715E-4	0.0	0.0	3.4051523952780687	0.0
24	2.5994191164747715E-4	0.0	0.0	3.512941641307889	0.0
25	3.465892155299696E-4	0.0	0.0	3.5976827045049666	0.0
26	3.465892155299696E-4	0.0	0.0	3.6885757262777012	0.0
27	3.465892155299696E-4	0.0	0.0	3.781634930647498	0.0
28	4.3323651941246197E-4	0.0	0.0	3.8841386911404863	0.0
29	4.3323651941246197E-4	0.0	0.0	4.000592667558556	0.0
30	4.3323651941246197E-4	0.0	0.0	4.139748237593839	0.0
31	5.198838232949543E-4	0.0	0.0	4.2617476414603885	0.0
32	5.198838232949543E-4	0.0	0.0	4.371356480871741	0.0
33	6.065311271774467E-4	0.0	0.0	4.4864241004276915	0.0
34	6.065311271774467E-4	0.0	0.0	4.607037147432121	0.0
35	6.065311271774467E-4	0.0	0.0	4.750525082661528	0.0
36	6.931784310599392E-4	0.0	0.0	4.86949183089219	0.0
37	6.931784310599392E-4	0.0	0.0	4.991231292847091	0.0
38	9.531203427074163E-4	0.0	0.0	5.135499053811442	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGT	80	0.0	44.000004	4
CGTTACA	20	7.856493E-4	44.000004	34
ACGTCAT	20	7.856493E-4	44.000004	15
ATATGCG	35	1.4460966E-7	44.0	1
ATGTACG	25	4.4428518E-5	44.0	1
GTTACGG	115	0.0	44.0	2
GTCGTAC	35	1.4460966E-7	44.0	9
CCGCAAT	55	1.8189894E-12	44.0	33
AAATGCG	55	1.8189894E-12	44.0	1
CGTTTTT	12305	0.0	43.213326	1
ATTTCGT	55	7.8216544E-11	40.0	38
CTCACGA	160	0.0	39.875004	24
TCGTAAG	50	1.3478711E-9	39.6	1
TGTAACG	50	1.3478711E-9	39.6	1
TTGTACG	45	2.3510438E-8	39.111107	1
GTTTTTT	14220	0.0	38.585094	2
TATCGCG	40	4.1245585E-7	38.500004	1
TACGGGA	220	0.0	38.0	4
CGGGATC	110	0.0	38.0	6
ACGTTAG	70	0.0	37.714287	1
>>END_MODULE