FastQCFastQC Report
Thu 26 May 2016
SRR1546039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546039_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1136108
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183811.6178919609755409No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC207.856442E-444.0000049
ATATGCG302.5276913E-644.01
TCGATAG351.4460602E-744.01
AATGCGT254.442808E-544.031
TCGACTA302.5276913E-644.01
ATCGCAC254.442808E-544.039
TAATCGC254.442808E-544.037
CGTTTTT116500.043.357941
ACGGTCT1050.041.90476230
ATACGAG900.041.5555571
TACGCGG603.6379788E-1240.3333322
GTTTTTT132950.039.317042
ATAGGGC4000.039.054
GGACCGA4450.038.56188
TCGTCCC3450.038.2608738
CGACTAT357.290135E-637.71428735
TTGGGAC3500.037.7142875
GTTGCGC700.037.71428741
TAGGGCA5400.037.481485
CTAACGG1000.037.3999982
Produced by FastQC (version 0.11.5)