##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546039_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1136108
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.74761114260264	31.0	28.0	31.0	16.0	33.0
2	28.54706770835167	31.0	28.0	33.0	16.0	33.0
3	28.03113612438254	31.0	26.0	33.0	16.0	34.0
4	32.17595950384999	35.0	32.0	35.0	26.0	37.0
5	33.60196477799646	35.0	33.0	35.0	28.0	37.0
6	33.44981286990321	35.0	33.0	37.0	28.0	37.0
7	34.38337728455393	35.0	35.0	37.0	31.0	37.0
8	34.78101289666123	35.0	35.0	37.0	32.0	37.0
9	36.597335816665314	39.0	37.0	39.0	32.0	39.0
10	36.31510120516712	38.0	35.0	39.0	32.0	39.0
11	36.33679016431537	38.0	35.0	39.0	32.0	39.0
12	35.117043450094535	37.0	34.0	39.0	29.0	39.0
13	35.21663697465382	37.0	34.0	39.0	28.0	39.0
14	35.860544948191546	38.0	34.0	40.0	27.0	41.0
15	36.41146792382414	38.0	35.0	40.0	31.0	41.0
16	36.05727712506205	37.0	34.0	40.0	30.0	41.0
17	35.595068426593244	37.0	34.0	40.0	29.0	41.0
18	35.25577057814926	37.0	33.0	39.0	29.0	40.0
19	34.71253965292032	36.0	33.0	39.0	27.0	40.0
20	35.29433293313664	36.0	34.0	39.0	30.0	40.0
21	35.40719016149873	36.0	34.0	39.0	30.0	40.0
22	35.74532702876839	36.0	34.0	40.0	31.0	41.0
23	36.11554183229059	37.0	35.0	40.0	31.0	41.0
24	35.70165952532682	36.0	34.0	40.0	31.0	41.0
25	35.38883275181585	35.0	34.0	40.0	30.0	41.0
26	35.543448334137246	36.0	34.0	40.0	30.0	41.0
27	35.8672564580128	36.0	35.0	40.0	31.0	41.0
28	35.78718748569678	36.0	35.0	40.0	31.0	41.0
29	35.93586525224715	37.0	35.0	40.0	31.0	41.0
30	35.59108817119499	36.0	35.0	40.0	30.0	41.0
31	34.857037359124305	36.0	34.0	40.0	29.0	41.0
32	34.89229985177465	36.0	34.0	40.0	28.0	41.0
33	34.67699285631296	36.0	34.0	40.0	25.0	41.0
34	34.35014628890915	36.0	34.0	40.0	23.0	41.0
35	34.11376295211371	36.0	34.0	40.0	22.0	41.0
36	33.77575019276336	36.0	33.0	40.0	20.0	41.0
37	33.70566706686336	36.0	33.0	40.0	20.0	41.0
38	33.55106556771011	36.0	33.0	40.0	18.0	41.0
39	33.32278973477874	35.0	33.0	40.0	18.0	41.0
40	33.1704406623314	35.0	33.0	40.0	18.0	40.0
41	32.896917370531675	35.0	32.0	39.0	18.0	40.0
42	33.111832677879214	35.0	33.0	39.0	18.0	40.0
43	32.920450344509504	35.0	33.0	39.0	18.0	40.0
44	32.769785090854036	35.0	32.0	39.0	17.0	40.0
45	32.72837353491041	35.0	32.0	39.0	18.0	40.0
46	32.54003756685104	35.0	32.0	39.0	17.0	40.0
47	32.544954352931235	35.0	32.0	39.0	17.0	40.0
48	32.539652040122945	35.0	32.0	39.0	17.0	40.0
49	32.57759561590976	35.0	32.0	39.0	18.0	40.0
50	32.655163945681224	35.0	32.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	5.0
12	9.0
13	25.0
14	50.0
15	108.0
16	285.0
17	642.0
18	1186.0
19	2074.0
20	3223.0
21	4710.0
22	6870.0
23	9578.0
24	13941.0
25	20805.0
26	28850.0
27	32995.0
28	32089.0
29	32707.0
30	36943.0
31	44818.0
32	59759.0
33	92040.0
34	146545.0
35	106438.0
36	108182.0
37	152056.0
38	163916.0
39	35254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.68257243149419	22.70479567083411	27.150323736827836	21.46230816084386
2	37.05730441120034	20.408711143658877	29.589352420720566	12.944632024420214
3	27.973308875564644	19.007083833579202	39.65934576642361	13.360261524432538
4	25.74508761490985	25.552764349868145	34.525854936326475	14.17629309889553
5	21.20326588669387	29.38030539350132	35.948078879824806	13.468349839980002
6	19.41980868016069	36.655846099138465	33.33837980192024	10.585965418780608
7	76.8650515619994	5.327662510958466	15.105430117559246	2.7018558094829013
8	78.66936946135402	3.3862097617480025	14.837057744510204	3.1073630323877657
9	75.13290989941098	4.82850221985938	16.284807430279518	3.753780450450133
10	46.30748133100022	21.242786777313423	21.395765191337443	11.05396670034891
11	39.547120520232234	20.210050452949897	27.130431261816657	13.112397765001216
12	35.942093533361266	20.222461244881647	30.2221267696381	13.613318452118989
13	21.113045590736093	33.130212972710346	30.971527354793732	14.78521408175983
14	15.719192189474946	34.430969590919176	34.53914592626757	15.31069229333831
15	15.109214968999426	21.427716379076635	48.5177465522644	14.945322099659538
16	18.128382160850904	17.88122255982706	46.66044073274724	17.329954546574797
17	17.95172642037553	18.48750294866333	34.188122960141115	29.37264767082003
18	21.864822710516957	20.814482425966546	38.156759744672165	19.163935118844336
19	26.2659007770388	22.507895376143814	32.12088991539537	19.105313931422014
20	27.80923996662289	22.231513201209744	31.116936065937395	18.84231076622997
21	22.390124882493563	25.031070989729848	33.694243857098094	18.88456027067849
22	21.80708172110398	22.21487745883314	32.32368753674827	23.654353283314613
23	19.23831185063392	26.1549078080605	32.35440644727438	22.2523738940312
24	20.075820256524906	21.68235766317991	41.02532505712485	17.216497023170334
25	18.66794354057889	21.941840036334572	38.59272181870034	20.797494604386202
26	16.918549997007325	28.468420255820746	33.70207761938126	20.91095212779067
27	17.707559492583453	28.373006791607843	34.52717523334049	19.392258482468215
28	15.791104366838363	25.210631383636063	40.635573378587246	18.362690870938327
29	17.21508870635538	22.793695669777872	39.69569794420953	20.295517679657216
30	19.83121322972816	25.566231379411114	36.053702640946106	18.54885274991462
31	25.788481376770516	23.405697345674884	31.643030416122407	19.16279086143219
32	27.38207987268816	23.32172645558345	31.6202332876804	17.675960384047997
33	25.974995334950552	24.035567041161578	30.148718255658792	19.840719368229077
34	19.635633232051884	25.625292665838106	32.834202382167895	21.90487171994212
35	20.30713629338056	24.72625841909396	33.89404880521922	21.07255648230626
36	26.80889492900323	24.750023765346253	29.44517598678999	18.995905318860533
37	20.832174405954362	29.461635689564723	30.500621419794598	19.205568484686314
38	21.243402915919965	30.15127082988589	27.8910983814919	20.714227872702242
39	21.250004400990047	28.338415009840613	29.040725001496337	21.370855587673002
40	23.504367542522367	24.70161287483232	28.70017639168107	23.09384319096424
41	18.466114137036268	23.85389417203294	30.062722910145865	27.617268780784926
42	21.92705270977759	24.600566143359607	27.442109376925433	26.030271769937364
43	21.449369250106503	24.82492861594144	29.01687163544311	24.708830498508945
44	20.077404612941727	27.299869378615416	30.082879444559847	22.53984656388301
45	18.5930386899837	32.07846437134498	26.891281462677846	22.43721547599348
46	21.4626602400476	28.50732500783376	28.928763814707757	21.101250937410878
47	21.07871786837167	26.23474176750802	29.72349459734462	22.963045766775693
48	22.281156368936756	23.495917641632662	32.17528615237284	22.047639837057744
49	21.519080932446563	23.253687149461143	33.00144000394329	22.225791914149006
50	20.267791442362874	28.73538431205484	29.958859545043254	21.037964700539032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	404.0
1	1058.0
2	1712.0
3	16160.5
4	30609.0
5	21409.5
6	12210.0
7	12119.5
8	12029.0
9	12348.5
10	12668.0
11	12594.5
12	12521.0
13	11832.5
14	11144.0
15	10308.0
16	9472.0
17	8927.0
18	8382.0
19	7855.0
20	7328.0
21	6755.5
22	6183.0
23	6066.0
24	5949.0
25	6303.5
26	6658.0
27	7673.0
28	8688.0
29	10163.5
30	11639.0
31	13941.0
32	16243.0
33	18309.5
34	20376.0
35	23200.0
36	26024.0
37	29432.0
38	32840.0
39	39572.0
40	46304.0
41	61758.5
42	77213.0
43	87968.0
44	98723.0
45	101074.0
46	103425.0
47	98781.0
48	94137.0
49	91192.5
50	88248.0
51	81995.0
52	75742.0
53	70295.5
54	64849.0
55	58080.5
56	51312.0
57	47669.5
58	44027.0
59	41368.0
60	38709.0
61	35114.5
62	31520.0
63	27778.0
64	24036.0
65	19608.5
66	15181.0
67	12779.0
68	10377.0
69	8474.0
70	6571.0
71	6020.5
72	5470.0
73	4323.0
74	3176.0
75	2486.5
76	1797.0
77	1368.5
78	940.0
79	750.5
80	561.0
81	467.0
82	373.0
83	269.5
84	166.0
85	119.5
86	73.0
87	57.5
88	42.0
89	33.5
90	25.0
91	20.5
92	16.0
93	11.0
94	6.0
95	6.0
96	6.0
97	3.0
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1136108.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.513635308510974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0069366825632	20.152202001185653
2	7.175734172010675	3.80509597815022
3	3.135396709229131	2.4939229468801978
4	2.037292558432726	2.1606412764411433
5	1.4685091383574171	1.946775787081212
6	1.123164391825753	1.786750264582412
7	0.914489583078209	1.6972510309417514
8	0.7650335390575159	1.622705620268037
9	0.5899205415332951	1.4076844289291779
>10	5.386598768661862	30.727892512753748
>50	1.1070723403170712	20.595944793914246
>100	0.2891806222133988	9.879074072595039
>500	0.0	0.0
>1k	3.354763598759883E-4	0.08912487256099494
>5k	0.0	0.0
>10k+	3.354763598759883E-4	1.6349344137162154
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18381	1.6178919609755409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044538019272815615	0.0
2	0.0	0.0	0.0	0.15852366148288718	0.0
3	8.80198009344182E-5	0.0	0.0	0.21300791826129206	0.0
4	1.760396018688364E-4	0.0	0.0	0.31493484774334835	0.0
5	1.760396018688364E-4	0.0	0.0	0.543434250969098	0.0
6	1.760396018688364E-4	0.0	0.0	0.7744862284219458	0.0
7	1.760396018688364E-4	0.0	0.0	0.9363546423403409	0.0
8	1.760396018688364E-4	0.0	0.0	1.3426540434536154	0.0
9	1.760396018688364E-4	0.0	0.0	1.5035542395617318	0.0
10	1.760396018688364E-4	0.0	0.0	1.763740771123872	0.0
11	1.760396018688364E-4	0.0	0.0	1.995056807979523	0.0
12	1.760396018688364E-4	0.0	0.0	2.194157597693177	0.0
13	2.6405940280325465E-4	0.0	0.0	2.2746076957472354	0.0
14	2.6405940280325465E-4	0.0	0.0	2.3032141310509213	0.0
15	2.6405940280325465E-4	0.0	0.0	2.3492484869396217	0.0
16	3.520792037376728E-4	0.0	0.0	2.452583733236629	0.0
17	3.520792037376728E-4	0.0	0.0	2.5726427417111752	0.0
18	3.520792037376728E-4	0.0	0.0	2.7585405612846667	0.0
19	3.520792037376728E-4	0.0	0.0	2.839782837547135	0.0
20	3.520792037376728E-4	0.0	0.0	2.9340520443478963	0.0
21	4.4009900467209106E-4	0.0	0.0	3.0559594686420657	0.0
22	4.4009900467209106E-4	0.0	0.0	3.175754417713809	0.0
23	4.4009900467209106E-4	0.0	0.0	3.3084882775229114	0.0
24	4.4009900467209106E-4	0.0	0.0	3.412263622824591	0.0
25	4.4009900467209106E-4	0.0	0.0	3.499491245550599	0.0
26	4.4009900467209106E-4	0.0	0.0	3.5900636207121153	0.0
27	4.4009900467209106E-4	0.0	0.0	3.676939164234386	0.0
28	4.4009900467209106E-4	0.0	0.0	3.7750812422762623	0.0
29	4.4009900467209106E-4	0.0	0.0	3.8811451024022365	0.0
30	4.4009900467209106E-4	0.0	0.0	4.021712724494503	0.0
31	4.4009900467209106E-4	0.0	0.0	4.137370742922328	0.0
32	4.4009900467209106E-4	0.0	0.0	4.24220232583522	0.0
33	4.4009900467209106E-4	0.0	0.0	4.354427572026603	0.0
34	4.4009900467209106E-4	0.0	0.0	4.475630837913297	0.0
35	4.4009900467209106E-4	0.0	0.0	4.616638559010235	0.0
36	4.4009900467209106E-4	0.0	0.0	4.734761131864224	0.0
37	4.4009900467209106E-4	0.0	0.0	4.847250437458411	0.0
38	4.4009900467209106E-4	0.0	0.0	4.985881623930119	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	20	7.856442E-4	44.000004	9
ATATGCG	30	2.5276913E-6	44.0	1
TCGATAG	35	1.4460602E-7	44.0	1
AATGCGT	25	4.442808E-5	44.0	31
TCGACTA	30	2.5276913E-6	44.0	1
ATCGCAC	25	4.442808E-5	44.0	39
TAATCGC	25	4.442808E-5	44.0	37
CGTTTTT	11650	0.0	43.35794	1
ACGGTCT	105	0.0	41.904762	30
ATACGAG	90	0.0	41.555557	1
TACGCGG	60	3.6379788E-12	40.333332	2
GTTTTTT	13295	0.0	39.31704	2
ATAGGGC	400	0.0	39.05	4
GGACCGA	445	0.0	38.5618	8
TCGTCCC	345	0.0	38.26087	38
CGACTAT	35	7.290135E-6	37.714287	35
TTGGGAC	350	0.0	37.714287	5
GTTGCGC	70	0.0	37.714287	41
TAGGGCA	540	0.0	37.48148	5
CTAACGG	100	0.0	37.399998	2
>>END_MODULE