FastQCFastQC Report
Thu 26 May 2016
SRR1546038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546038_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences972526
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96770.995037664802792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGA207.855883E-444.012
ATTCGTT254.4423337E-544.038
ATTCGGC254.4423337E-544.017
GTTACGG1000.044.02
AATGCGT207.855883E-444.031
CTAACGG750.044.02
GTCGTAC207.855883E-444.09
TGCGTTA302.527313E-644.018
CGTTTTT106600.043.3808631
CATACGA2450.041.3061218
CGACGGT1500.041.06666628
TTACGAC750.041.06666624
ATAGGCG2450.040.408164
GTTGATC3550.039.66197616
ATCCTGC3150.039.1111120
CGGGAAT13950.039.111119
TAGGCGG5100.038.8235325
ATTTCGT850.038.82353238
GTTTTTT122400.038.823532
TCGTCCC2900.038.68965538
Produced by FastQC (version 0.11.5)