##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546038_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	972526
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.59430596199999	28.0	19.0	30.0	16.0	33.0
2	25.88986207052562	30.0	25.0	31.0	10.0	33.0
3	26.216878520471433	30.0	25.0	31.0	16.0	33.0
4	28.28848997353284	32.0	19.0	35.0	10.0	37.0
5	31.11485451288706	33.0	30.0	35.0	19.0	37.0
6	29.646293261054204	33.0	28.0	35.0	17.0	37.0
7	29.587382753777277	32.0	28.0	35.0	17.0	35.0
8	32.34591774410144	33.0	32.0	35.0	28.0	35.0
9	26.916019725950772	30.0	23.0	34.0	10.0	35.0
10	31.369771090952838	34.0	30.0	37.0	17.0	37.0
11	33.53226648953344	35.0	32.0	37.0	27.0	39.0
12	32.927050793500634	35.0	31.0	37.0	23.0	39.0
13	32.984073433512314	35.0	31.0	37.0	22.0	39.0
14	32.62540950062004	35.0	30.0	38.0	21.0	40.0
15	31.626001772703248	34.0	27.0	38.0	18.0	40.0
16	30.277541165994535	33.0	26.0	37.0	17.0	40.0
17	30.376989406967013	32.0	25.0	37.0	17.0	40.0
18	31.323458704445947	34.0	27.0	37.0	18.0	39.0
19	30.995761552904497	34.0	27.0	37.0	18.0	39.0
20	31.769681221890213	34.0	29.0	37.0	19.0	39.0
21	32.59837886082223	34.0	31.0	37.0	23.0	39.0
22	32.98632735782899	34.0	31.0	37.0	25.0	39.0
23	34.282655682213125	35.0	33.0	38.0	28.0	40.0
24	33.932731875548825	35.0	33.0	38.0	27.0	40.0
25	32.71730730078168	35.0	31.0	38.0	23.0	39.0
26	26.434174510501517	33.0	20.0	36.0	0.0	39.0
27	29.653932131377463	34.0	24.0	37.0	15.0	39.0
28	31.128476770800987	34.0	27.0	37.0	19.0	39.0
29	32.45568550352381	34.0	30.0	37.0	23.0	39.0
30	32.20396061390647	34.0	30.0	37.0	22.0	39.0
31	31.010026467158717	33.0	29.0	36.0	18.0	38.0
32	31.392531407900663	34.0	30.0	37.0	18.0	39.0
33	31.192976845863246	34.0	30.0	37.0	18.0	39.0
34	31.244456189346096	34.0	30.0	37.0	17.0	39.0
35	30.504927374692297	34.0	29.0	37.0	13.0	39.0
36	30.29138449768952	34.0	27.0	37.0	12.0	39.0
37	30.361632491059364	34.0	28.0	37.0	10.0	39.0
38	30.08684497895172	33.0	27.0	37.0	10.0	39.0
39	29.993333854313406	34.0	27.0	37.0	10.0	39.0
40	29.917672123932935	33.0	27.0	36.0	10.0	38.0
41	29.34288234967497	33.0	26.0	36.0	10.0	38.0
42	29.5185167286016	33.0	26.0	36.0	10.0	38.0
43	29.072826844732173	33.0	26.0	36.0	10.0	38.0
44	28.883098241075302	33.0	25.0	35.0	10.0	38.0
45	28.388777266623205	32.0	24.0	35.0	10.0	38.0
46	27.984157750024163	31.0	23.0	35.0	9.0	38.0
47	28.107102535047908	32.0	23.0	35.0	10.0	38.0
48	28.165518454005344	32.0	23.0	35.0	10.0	38.0
49	27.966089338485553	31.0	24.0	35.0	9.0	38.0
50	29.785875133415455	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	11.0
11	22.0
12	65.0
13	135.0
14	349.0
15	803.0
16	1564.0
17	2809.0
18	4597.0
19	7086.0
20	10469.0
21	14073.0
22	18989.0
23	24845.0
24	31753.0
25	39564.0
26	46859.0
27	52766.0
28	59582.0
29	69981.0
30	84285.0
31	98260.0
32	105804.0
33	103776.0
34	89073.0
35	66070.0
36	32269.0
37	6487.0
38	178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.787767113681276	23.539730557332142	26.829616894561177	22.8428854344254
2	36.981324920876155	17.56981304355873	31.456845369686775	13.992016665878342
3	29.30574606745732	14.605779177111975	41.641765875668106	14.446708879762598
4	27.068582228135803	25.342972835687682	33.59437177000923	13.99407316616728
5	20.331487281573963	29.65936129214026	36.864721354493355	13.144430071792426
6	19.417064428097554	35.08111865389717	34.81007191581511	10.691745002190173
7	79.47684689149699	3.7946543331489337	14.97245317862967	1.7560455967244062
8	81.94505853828072	1.838202783267491	14.363214968031704	1.8535237104200815
9	69.53942619528938	2.814217820397604	14.66161315995665	12.984742824356365
10	47.76314463572182	20.7567715413264	21.40827083286205	10.071812990089725
11	40.90430487205484	19.629706557973773	27.251610753851313	12.214377816120082
12	36.84559590180622	20.004915035690562	30.544376191484858	12.60511287101836
13	20.526957634037547	33.98623790006642	31.243277814680532	14.243526651215493
14	14.908187544600349	34.74940515729143	35.432677378291174	14.909729919817053
15	13.642720091802174	19.694280667046435	49.09668224808386	17.566316993067538
16	16.19123807486895	15.783228417543594	47.30176879589851	20.72376471168894
17	16.991216687265943	17.42493259820303	35.12008933437255	30.463761380158473
18	21.405186082428646	20.184961635987108	39.428251789669375	18.981600491914868
19	26.891928853316006	21.596440609299904	33.036648891649165	18.47498164573492
20	28.455486022995785	21.587392008028576	31.747223210484858	18.209898758490777
21	22.074268451434715	24.765404729539362	34.46550529240349	18.694821526622423
22	21.419890059494552	21.81597201514407	32.74071850007095	24.02341942529043
23	18.54767893094889	26.012569329765988	32.993668035610355	22.44608370367476
24	19.329149040745442	21.018358378079352	43.10661103147885	16.545881549696357
25	18.057614912094895	20.5218163833152	40.544108846447294	20.87645985814261
26	13.374346804095726	40.47644998694122	28.337545731425173	17.811657477537874
27	16.739501051899897	29.33381729640133	35.23309402524971	18.693587626449062
28	15.069417167253112	24.95779033156954	42.30642676905296	17.666365732124387
29	16.42177175725893	22.371124268142957	41.13381030430035	20.07329367029776
30	19.322568239820836	25.451556050943623	37.04384252965988	18.18203317957566
31	26.42582306282814	22.9995907564425	32.207982100221486	18.366604080507873
32	27.86588739015718	22.58705679848148	32.224948227605225	17.322107583756114
33	26.37852355618256	23.67916127692216	30.420883349134108	19.52143181776117
34	19.374083572058744	25.1325928561293	32.97032675733091	22.522996814481054
35	20.080182946265705	24.236164380181098	34.27733551596564	21.40631715758756
36	27.07403195390149	24.157297594100314	29.512527171510065	19.256143280488132
37	20.293544851243052	30.011536966620945	30.2330220477396	19.461896134396405
38	20.67255785449438	30.82447153083825	27.652114185122045	20.850856429545328
39	20.948128893212107	28.537848859567767	28.937735340751814	21.57628690646831
40	23.68152625225444	24.104856836732385	28.63460719816231	23.579009712850866
41	17.915407917114813	23.190331158241527	30.184385815906207	28.709875108737453
42	21.394183805882825	23.927483686811456	26.827560394272236	27.850772113033486
43	21.005299601244594	24.16408404505381	28.9999444744922	25.830671879209397
44	19.349097093548142	26.982003565971503	30.45687210419053	23.212027236289824
45	17.91551074212926	33.047548343180544	26.44813609096312	22.588804823727077
46	20.830291426655943	28.259604370474413	28.64046822398579	22.269635978883855
47	20.680372555592346	25.762704544659986	29.243639758731387	24.31328314101628
48	21.61196718648139	22.882370239973017	32.848993240283555	22.65666933326204
49	21.073883885880687	23.06426769052961	32.84467458967678	23.017173833912924
50	19.8320661864053	29.34348284775934	29.8713864719298	20.95306449390556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	232.0
1	627.5
2	1023.0
3	9428.5
4	17834.0
5	14204.5
6	10575.0
7	10436.0
8	10297.0
9	10887.5
10	11478.0
11	11772.0
12	12066.0
13	11896.5
14	11727.0
15	11304.5
16	10882.0
17	10323.5
18	9765.0
19	9258.5
20	8752.0
21	8194.5
22	7637.0
23	7169.0
24	6701.0
25	6946.5
26	7192.0
27	7747.0
28	8302.0
29	9464.0
30	10626.0
31	12567.5
32	14509.0
33	16147.5
34	17786.0
35	19890.0
36	21994.0
37	24560.0
38	27126.0
39	32129.5
40	37133.0
41	49822.0
42	62511.0
43	71769.5
44	81028.0
45	83746.0
46	86464.0
47	83019.0
48	79574.0
49	75887.0
50	72200.0
51	67950.0
52	63700.0
53	59527.5
54	55355.0
55	50026.0
56	44697.0
57	41713.0
58	38729.0
59	36327.5
60	33926.0
61	31189.0
62	28452.0
63	24840.0
64	21228.0
65	17525.0
66	13822.0
67	11665.5
68	9509.0
69	7851.5
70	6194.0
71	5526.5
72	4859.0
73	3839.0
74	2819.0
75	2226.0
76	1633.0
77	1290.0
78	947.0
79	759.5
80	572.0
81	457.0
82	342.0
83	252.0
84	162.0
85	118.0
86	74.0
87	59.0
88	44.0
89	36.5
90	29.0
91	21.0
92	13.0
93	9.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	972526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.93569116909174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.83926113788704	35.1382524172297
2	7.109396259065816	6.10493684355892
3	2.8274924660203085	3.642010299119447
4	1.5468664370380174	2.6566311848199042
5	1.0546528341104102	2.2641124187985957
6	0.7356543897194037	1.8951497810507385
7	0.5733258881003459	1.7231300289505191
8	0.43452125748931575	1.4925176414373322
9	0.3622379745851244	1.3997644025853784
>10	3.0599735403491994	27.91761573219261
>50	0.40707064620535266	11.841045290489006
>100	0.04928639485367618	2.8413451075221654
>500	0.0	0.0
>1k	0.0	0.0
>5k	2.607745759202054E-4	1.0834888522457125
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9677	0.995037664802792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.030539029290733617	0.0
2	0.0	0.0	0.0	0.12585781768302337	0.0
3	0.0	0.0	0.0	0.16647369838955461	0.0
4	0.0	0.0	0.0	0.2306365074044293	0.0
5	0.0	0.0	0.0	0.3952593555339394	0.0
6	0.0	0.0	0.0	0.5292403493582691	0.0
7	0.0	0.0	0.0	0.6298032134873515	0.0
8	0.0	0.0	0.0	0.8832668740989958	0.0
9	0.0	0.0	0.0	1.0108727170276168	0.0
10	0.0	0.0	0.0	1.1954436179598282	0.0
11	0.0	0.0	0.0	1.356981715655931	0.0
12	0.0	0.0	0.0	1.4877751340324064	0.0
13	0.0	0.0	0.0	1.5456656171660192	1.0282501444691454E-4
14	0.0	0.0	0.0	1.562734569564207	1.0282501444691454E-4
15	0.0	0.0	0.0	1.5925538237538122	1.0282501444691454E-4
16	0.0	0.0	0.0	1.6502386568585312	1.0282501444691454E-4
17	0.0	0.0	0.0	1.7439122450196705	1.0282501444691454E-4
18	0.0	0.0	0.0	1.8631892617780912	1.0282501444691454E-4
19	0.0	0.0	0.0	1.9091520432358622	1.0282501444691454E-4
20	0.0	0.0	0.0	1.9697159767450947	1.0282501444691454E-4
21	0.0	0.0	0.0	2.0390200364823152	1.0282501444691454E-4
22	0.0	0.0	0.0	2.111408846652943	1.0282501444691454E-4
23	0.0	0.0	0.0	2.188527607488129	1.0282501444691454E-4
24	0.0	0.0	0.0	2.248783065954021	1.0282501444691454E-4
25	0.0	0.0	0.0	2.302766198538651	1.0282501444691454E-4
26	0.0	0.0	0.0	2.354281530776555	1.0282501444691454E-4
27	0.0	0.0	0.0	2.4183415147769827	1.0282501444691454E-4
28	0.0	0.0	0.0	2.490216199875376	1.0282501444691454E-4
29	0.0	0.0	0.0	2.569699936042841	1.0282501444691454E-4
30	0.0	0.0	0.0	2.669337375041901	1.0282501444691454E-4
31	0.0	0.0	0.0	2.76568441357866	1.0282501444691454E-4
32	0.0	0.0	0.0	2.8448596747027843	1.0282501444691454E-4
33	0.0	0.0	0.0	2.9335976621704716	1.0282501444691454E-4
34	0.0	0.0	0.0	3.024392149927097	1.0282501444691454E-4
35	0.0	0.0	0.0	3.1298906147496313	1.0282501444691454E-4
36	0.0	0.0	0.0	3.2198625023906815	1.0282501444691454E-4
37	0.0	0.0	0.0	3.3146671657107367	1.0282501444691454E-4
38	0.0	0.0	0.0	3.411836804363071	1.0282501444691454E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGA	20	7.855883E-4	44.0	12
ATTCGTT	25	4.4423337E-5	44.0	38
ATTCGGC	25	4.4423337E-5	44.0	17
GTTACGG	100	0.0	44.0	2
AATGCGT	20	7.855883E-4	44.0	31
CTAACGG	75	0.0	44.0	2
GTCGTAC	20	7.855883E-4	44.0	9
TGCGTTA	30	2.527313E-6	44.0	18
CGTTTTT	10660	0.0	43.380863	1
CATACGA	245	0.0	41.30612	18
CGACGGT	150	0.0	41.066666	28
TTACGAC	75	0.0	41.066666	24
ATAGGCG	245	0.0	40.40816	4
GTTGATC	355	0.0	39.661976	16
ATCCTGC	315	0.0	39.11111	20
CGGGAAT	1395	0.0	39.11111	9
TAGGCGG	510	0.0	38.823532	5
ATTTCGT	85	0.0	38.823532	38
GTTTTTT	12240	0.0	38.82353	2
TCGTCCC	290	0.0	38.689655	38
>>END_MODULE