FastQCFastQC Report
Thu 26 May 2016
SRR1546037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546037_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1381124
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217761.5766868145076038No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG207.857033E-444.0000041
TCTAGCG351.4463694E-744.01
ATCCGTT254.443307E-544.031
GTAATCG254.443307E-544.01
GCGTAAG600.044.01
CGTTTTT133250.042.976361
ACGGGAC1800.041.5555575
TAGACGG1400.040.8571432
TCGATAG700.040.8571431
AGTACGG1050.039.8095252
GTACGAG1300.038.9230771
GTTTTTT154200.038.5499342
CAACCCG404.1253406E-738.50000423
GCGAGAC1950.038.35897421
CGTTAGG3300.038.02
CGTAAGG1450.037.9310342
TTACGAG357.2912753E-637.7142871
TACGGGA2000.037.44
TAGGGTA6500.037.230775
TGCGTAG950.037.0526351
Produced by FastQC (version 0.11.5)