FastQCFastQC Report
Thu 26 May 2016
SRR1546036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546036_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1389452
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227951.6405748453347075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG551.8189894E-1244.0000041
TCGCTAA302.5281006E-644.02
CGACGGT2050.042.92682628
CGTTTTT137950.042.89961
AGTACGG1500.042.5333332
TCGTAAG750.041.0666661
CGGTCTA2100.040.85714331
CGTTAGG2600.040.6153832
CGTAAGG1900.040.5263142
TCACGAC2200.040.00000425
AGGGTAC6050.039.2727286
CACAACG2850.038.59649312
TACGTAG800.038.51
GTAGGGT5950.038.453784
GTTTTTT159350.038.4085352
TAGGGTA6650.038.375945
TAGACGG1150.038.260872
ATGCGTC357.2913062E-637.71428728
ACGGTCT2300.037.30434830
ACGTTAG651.0913936E-1137.2307661
Produced by FastQC (version 0.11.5)