##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546036_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1389452
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.887021645943868	31.0	28.0	33.0	22.0	33.0
2	28.63444365116607	31.0	28.0	33.0	16.0	33.0
3	28.081701994743252	31.0	26.0	33.0	16.0	34.0
4	32.25723306742514	35.0	32.0	35.0	26.0	37.0
5	33.673536041547315	35.0	33.0	35.0	28.0	37.0
6	33.502812619651486	35.0	33.0	37.0	28.0	37.0
7	34.50057936510221	35.0	35.0	37.0	32.0	37.0
8	34.83604327461474	35.0	35.0	37.0	32.0	37.0
9	36.61557794007997	39.0	37.0	39.0	32.0	39.0
10	36.106200861922545	38.0	35.0	39.0	31.0	39.0
11	36.278270138155186	38.0	35.0	39.0	31.0	39.0
12	34.91479374602361	37.0	33.0	39.0	28.0	39.0
13	35.29565109122157	37.0	34.0	39.0	29.0	39.0
14	35.94348419376848	38.0	34.0	40.0	28.0	41.0
15	36.53981785624836	38.0	35.0	40.0	31.0	41.0
16	36.13150508257932	38.0	34.0	40.0	31.0	41.0
17	35.750577205977606	37.0	34.0	40.0	30.0	41.0
18	35.437515653653385	37.0	33.0	39.0	29.0	40.0
19	34.934258254333365	37.0	33.0	39.0	27.0	41.0
20	35.29158545959126	36.0	34.0	39.0	29.0	41.0
21	35.442750811111146	36.0	34.0	39.0	30.0	40.0
22	35.89937903576374	37.0	34.0	40.0	31.0	41.0
23	36.28058328031483	37.0	35.0	40.0	31.0	41.0
24	35.83130255669141	36.0	34.0	40.0	31.0	41.0
25	35.54911648621183	36.0	34.0	40.0	30.0	41.0
26	35.70855128496702	37.0	34.0	40.0	30.0	41.0
27	36.03737372719605	37.0	35.0	40.0	31.0	41.0
28	35.938562109378374	37.0	35.0	40.0	31.0	41.0
29	36.07835751073085	37.0	35.0	40.0	31.0	41.0
30	35.74770197171259	37.0	35.0	40.0	30.0	41.0
31	35.048626364926605	36.0	34.0	40.0	29.0	41.0
32	35.08671548207495	37.0	34.0	40.0	29.0	41.0
33	34.89019196057151	37.0	34.0	40.0	27.0	41.0
34	34.56703074305553	37.0	34.0	40.0	24.0	41.0
35	34.39025745401784	37.0	34.0	40.0	23.0	41.0
36	33.99346001157291	36.0	33.0	40.0	21.0	41.0
37	33.92089543215599	36.0	33.0	40.0	21.0	41.0
38	33.77890636020532	36.0	33.0	40.0	20.0	41.0
39	33.53685913583197	36.0	33.0	40.0	19.0	41.0
40	33.34155983797929	36.0	33.0	40.0	18.0	40.0
41	33.016378399541686	35.0	33.0	39.0	18.0	40.0
42	33.19095154060738	36.0	33.0	40.0	18.0	40.0
43	32.98434706632543	35.0	33.0	39.0	18.0	40.0
44	32.86021683368695	35.0	32.0	39.0	17.0	40.0
45	32.80365784496334	35.0	32.0	39.0	18.0	40.0
46	32.626731977786925	35.0	32.0	39.0	18.0	40.0
47	32.57180600697253	35.0	32.0	39.0	18.0	40.0
48	32.57564493051937	35.0	32.0	39.0	18.0	40.0
49	32.6147790639763	35.0	32.0	39.0	18.0	40.0
50	32.803327498898845	35.0	32.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	6.0
11	4.0
12	10.0
13	21.0
14	52.0
15	143.0
16	355.0
17	744.0
18	1464.0
19	2460.0
20	3910.0
21	5731.0
22	8054.0
23	11424.0
24	16385.0
25	24304.0
26	33752.0
27	38826.0
28	38780.0
29	38914.0
30	43960.0
31	53219.0
32	71520.0
33	108211.0
34	166425.0
35	130825.0
36	142483.0
37	199156.0
38	209402.0
39	38910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.495766676358734	22.184501515705474	28.08891563004695	21.230816177888837
2	35.56366106925608	20.85412090522019	30.19341438207293	13.3888036434508
3	27.76691818069282	19.758725022526864	38.75938139640664	13.714975400373671
4	25.0852134510584	25.60728978043142	34.86007433146305	14.447422437047125
5	20.993888237952802	29.42174324841736	35.86716201783149	13.717206495798342
6	19.44262918042509	37.25000935620662	32.82970552419227	10.47765593917602
7	78.0570325567202	4.756479532938165	14.565022757173333	2.6214651531682995
8	79.41720908674786	3.396806798651555	14.177243978201478	3.008740136399098
9	75.81183085129965	4.84608320402576	15.594205485328027	3.747880459346563
10	43.53666049636835	21.901656192513308	22.02069592904253	12.54098738207581
11	37.622098496385625	21.66652752308104	27.105218460227487	13.606155520305846
12	33.80728517429893	20.419920947251146	30.565431551431786	15.207362327018133
13	21.443777834714695	31.127091831887682	31.3520726156787	16.07705771771893
14	16.884210465708783	32.42127111983717	34.17390453214649	16.520613882307558
15	16.259575717621047	21.94224773507829	45.80359738947441	15.99457915782625
16	18.700322141390995	18.97352337468297	43.65965862800586	18.666495855920175
17	18.912995914936246	19.348851201768756	33.950723018859236	27.787429864435765
18	22.333409142597223	21.718922280150736	36.69820907811137	19.24945949914067
19	25.845513195130167	23.402895530036304	31.147027749069416	19.604563525764114
20	27.15235934742618	22.90831205396084	30.3808263977453	19.558502200867682
21	21.784631638948305	25.53438333961878	33.104993911268615	19.575991110164296
22	21.71949804671194	21.747278783290103	32.89865356989662	23.634569600101337
23	19.76952064554947	25.930438762907965	32.12208842047081	22.17795217107176
24	19.887408848956277	22.24272590920737	39.12758411229751	18.74228112953884
25	19.738429251244373	22.356655717505895	37.06425266939772	20.84066236185201
26	17.803637693133695	28.040407297265396	32.50216632168654	21.65378868791437
27	18.164355443728894	27.589150254920646	34.27387200133578	19.97262230001468
28	16.7690571534677	24.89074829501127	39.08504935758846	19.25514519393257
29	17.569876469284292	23.832345413875398	37.86406439373221	20.733713723108103
30	20.451732049757744	24.610853775445282	35.61260122695854	19.324812947838428
31	25.810247493256334	23.137611086960902	31.351856703218246	19.700284716564518
32	26.462878890382687	23.724964950210587	31.16962658659673	18.64252957281
33	24.755443153127995	24.398827739281387	30.12007611633939	20.725652991251227
34	19.213690001525784	25.45125704234475	32.78601923636081	22.54903371976866
35	20.31210865866543	25.27715962840026	32.657623293212	21.75310841972231
36	25.871854515305316	25.10191068133336	29.095499520674338	19.93073528268699
37	20.208254765188002	29.40994003391265	30.033135365597374	20.348669835301976
38	22.039336371461555	28.927879480543407	28.078120007024353	20.954664140970685
39	21.105443009186356	27.772747817125026	28.732262791373863	22.389546382314755
40	24.001045016308588	24.493541338599677	28.87735596479763	22.6280576802941
41	19.627090392471278	23.309189522200118	29.938421766279077	27.125298319049524
42	22.293465337413597	24.364713570529965	27.92079179417497	25.421029297881464
43	21.71417220602079	24.717082705987682	28.785089373364464	24.783655714627063
44	20.729827298819966	27.193958481473274	30.009600907408103	22.06661331229866
45	19.50977795562567	30.407671513661498	27.01547084749959	23.067079683213237
46	21.374685847370042	28.26250924825039	29.127454564821235	21.23535033955833
47	21.073056140118553	25.691063815086814	30.07005639633467	23.16582364845997
48	21.692077164234533	24.64000195760631	31.8721337620875	21.79578711607166
49	21.338844378935004	23.761526126847134	32.29093196454429	22.60869752967357
50	19.315672653679293	28.071282779110035	30.160595688084225	22.452448879126447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	570.0
1	1295.0
2	2020.0
3	18779.0
4	35538.0
5	24614.0
6	13690.0
7	13500.5
8	13311.0
9	13897.0
10	14483.0
11	14317.5
12	14152.0
13	13412.5
14	12673.0
15	11814.5
16	10956.0
17	10249.5
18	9543.0
19	8926.0
20	8309.0
21	7781.5
22	7254.0
23	7227.5
24	7201.0
25	7691.5
26	8182.0
27	9833.5
28	11485.0
29	13574.5
30	15664.0
31	18369.5
32	21075.0
33	24094.0
34	27113.0
35	29253.0
36	31393.0
37	35810.0
38	40227.0
39	48662.5
40	57098.0
41	71075.0
42	85052.0
43	99087.0
44	113122.0
45	117756.5
46	122391.0
47	119098.5
48	115806.0
49	114881.0
50	113956.0
51	106194.5
52	98433.0
53	87717.5
54	77002.0
55	71199.0
56	65396.0
57	62901.0
58	60406.0
59	55284.5
60	50163.0
61	44446.0
62	38729.0
63	33110.0
64	27491.0
65	23448.5
66	19406.0
67	16798.5
68	14191.0
69	11696.0
70	9201.0
71	8009.5
72	6818.0
73	5582.0
74	4346.0
75	3580.5
76	2815.0
77	2016.0
78	1217.0
79	1017.5
80	818.0
81	576.0
82	334.0
83	284.5
84	235.0
85	159.0
86	83.0
87	62.0
88	41.0
89	38.0
90	35.0
91	22.0
92	9.0
93	9.5
94	10.0
95	7.5
96	5.0
97	4.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1389452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.795100927069594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.375631658949	20.4649275867023
2	7.373763328937392	3.951614652224041
3	3.0387318804543697	2.4426938228123642
4	1.9038246416068723	2.0405269367719296
5	1.4046640050783805	1.8819056892348502
6	1.0835785067216304	1.7420757270005667
7	0.8433896668970227	1.5819097871747325
8	0.711445213781946	1.5250597045894276
9	0.5691567317981462	1.3725550864667166
>10	5.323463393243574	31.003685652716495
>50	1.1055007340714982	20.772679803617592
>100	0.26549978178997113	9.381122716428528
>500	8.102740020407094E-4	0.11485316667865281
>1k	2.7009133401356987E-4	0.07468712540161922
>5k	0.0	0.0
>10k+	2.7009133401356987E-4	1.6497025421801452
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22795	1.6405748453347075	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.197082015067812E-5	0.0	0.0	0.036489205816393804	0.0
2	7.197082015067812E-5	0.0	0.0	0.13861579961020604	0.0
3	7.197082015067812E-5	0.0	0.0	0.19209011898215989	0.0
4	7.197082015067812E-5	0.0	0.0	0.30918664336731316	0.0
5	1.4394164030135623E-4	0.0	0.0	0.5613004263551385	0.0
6	1.4394164030135623E-4	0.0	0.0	0.8306872061791267	0.0
7	1.4394164030135623E-4	0.0	0.0	1.035444189507806	0.0
8	1.4394164030135623E-4	0.0	0.0	1.5755851947386452	0.0
9	1.4394164030135623E-4	0.0	0.0	1.8138086094373898	0.0
10	2.1591246045203432E-4	0.0	0.0	2.18848869914182	0.0
11	2.1591246045203432E-4	0.0	0.0	2.458739128807616	0.0
12	2.1591246045203432E-4	0.0	0.0	2.7236637177822622	0.0
13	2.8788328060271246E-4	0.0	0.0	2.8244228659932116	0.0
14	2.8788328060271246E-4	0.0	0.0	2.859040830485688	0.0
15	2.8788328060271246E-4	0.0	0.0	2.9096363170516146	0.0
16	2.8788328060271246E-4	0.0	0.0	3.0117629108454267	0.0
17	3.5985410075339055E-4	0.0	0.0	3.1292192893313335	0.0
18	3.5985410075339055E-4	0.0	0.0	3.323180649637411	0.0
19	3.5985410075339055E-4	0.0	0.0	3.4024205226233075	0.0
20	3.5985410075339055E-4	0.0	0.0	3.4992212757259695	0.0
21	3.5985410075339055E-4	0.0	0.0	3.6195564870179036	0.0
22	3.5985410075339055E-4	0.0	0.0	3.744209947518878	0.0
23	3.5985410075339055E-4	0.0	0.0	3.8766362565961257	0.0
24	4.3182492090406864E-4	0.0	0.0	3.9754521926630066	0.0
25	4.3182492090406864E-4	0.0	0.0	4.058938344037793	0.0
26	4.3182492090406864E-4	0.0	0.0	4.143863911815593	0.0
27	4.3182492090406864E-4	0.0	0.0	4.235194882586804	0.0
28	5.037957410547468E-4	0.0	0.0	4.325662203516206	0.0
29	5.037957410547468E-4	0.0	0.0	4.433762375382525	0.0
30	5.037957410547468E-4	0.0	0.0	4.57554489107936	0.0
31	5.037957410547468E-4	0.0	0.0	4.692929298745117	0.0
32	5.037957410547468E-4	0.0	0.0	4.803332536856257	0.0
33	5.037957410547468E-4	0.0	0.0	4.916686578593575	0.0
34	5.757665612054249E-4	0.0	0.0	5.035870256763098	0.0
35	5.757665612054249E-4	0.0	0.0	5.170527661265017	0.0
36	5.757665612054249E-4	0.0	0.0	5.288271923031526	0.0
37	5.757665612054249E-4	0.0	0.0	5.409758667445871	0.0
38	5.757665612054249E-4	0.0	0.0	5.573348341648362	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	55	1.8189894E-12	44.000004	1
TCGCTAA	30	2.5281006E-6	44.0	2
CGACGGT	205	0.0	42.926826	28
CGTTTTT	13795	0.0	42.8996	1
AGTACGG	150	0.0	42.533333	2
TCGTAAG	75	0.0	41.066666	1
CGGTCTA	210	0.0	40.857143	31
CGTTAGG	260	0.0	40.615383	2
CGTAAGG	190	0.0	40.526314	2
TCACGAC	220	0.0	40.000004	25
AGGGTAC	605	0.0	39.272728	6
CACAACG	285	0.0	38.596493	12
TACGTAG	80	0.0	38.5	1
GTAGGGT	595	0.0	38.45378	4
GTTTTTT	15935	0.0	38.408535	2
TAGGGTA	665	0.0	38.37594	5
TAGACGG	115	0.0	38.26087	2
ATGCGTC	35	7.2913062E-6	37.714287	28
ACGGTCT	230	0.0	37.304348	30
ACGTTAG	65	1.0913936E-11	37.230766	1
>>END_MODULE