FastQCFastQC Report
Thu 26 May 2016
SRR1546035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546035_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1369111
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224111.6369016098767741No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAC302.5280733E-644.00000412
GTACGAC302.5280733E-644.00000434
TTACGAG502.7284841E-1144.01
ACCGTAT207.85701E-444.039
TCGCGAC254.4432876E-544.043
TATAGCG650.043.9999961
CGTTTTT138350.043.0299951
GCGTAAG1000.041.81
ATCGCCA501.3478711E-939.624
TAGGGTA5750.039.0260855
TGCGGGT1700.038.8235324
GTTTTTT159050.038.7299582
CGTAAGG2450.038.6122442
CGGTCTA2000.038.531
TACGTAG404.1253043E-738.51
CGTTAGG2750.038.42
CACAACG2900.037.93103812
AACGTAA357.29123E-637.71428719
TCGATAG700.037.7142871
GTATGCG357.29123E-637.7142871
Produced by FastQC (version 0.11.5)