FastQCFastQC Report
Thu 26 May 2016
SRR1546034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546034_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1179750
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119391.011994066539521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAG650.044.0000041
GGTACGA207.8565674E-444.038
TAAGTCG254.442912E-544.018
CGTTTTT124350.043.1684761
ACGGTCT1500.042.53333330
CGATAGG1450.040.965522
CGGTATA557.8216544E-1140.043
CGGTCTA1600.039.87531
ATACCGG452.3510438E-839.111112
GTTTTTT140800.039.1093752
CGTAGGC850.038.823533
CGGAATT4200.038.7619069
CGACGGT1650.038.66666828
TAGGGTA5600.038.55
GGCGGGA18500.037.8162167
CTATTCG357.2903695E-637.71428744
CTCGTCC3100.037.612937
GACGGTC1700.037.5294129
CGGGAAT16200.037.481489
ATCGTAG601.9826984E-1036.6666641
Produced by FastQC (version 0.11.5)