##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546034_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1179750
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.594587836406017	28.0	23.0	30.0	16.0	33.0
2	25.845008688281414	30.0	23.0	31.0	10.0	33.0
3	26.137377410468318	30.0	25.0	31.0	16.0	33.0
4	28.243682136045773	32.0	19.0	35.0	10.0	37.0
5	31.11888111888112	33.0	30.0	35.0	19.0	37.0
6	29.59650010595465	33.0	28.0	35.0	17.0	37.0
7	29.539771985590168	32.0	28.0	35.0	17.0	35.0
8	32.35083958465777	33.0	32.0	35.0	28.0	35.0
9	26.84156982411528	30.0	23.0	34.0	10.0	35.0
10	31.017318075863532	34.0	27.0	37.0	17.0	37.0
11	33.39323755032846	35.0	32.0	37.0	27.0	39.0
12	32.826648018648015	35.0	31.0	37.0	23.0	39.0
13	33.12795931341386	35.0	31.0	37.0	23.0	39.0
14	32.77862089425726	35.0	30.0	38.0	21.0	40.0
15	31.684687433778343	34.0	27.0	38.0	18.0	40.0
16	30.250038567493114	33.0	26.0	37.0	17.0	40.0
17	30.410141131595676	32.0	26.0	37.0	17.0	40.0
18	31.33565840220386	34.0	27.0	37.0	17.0	39.0
19	30.996914600550966	34.0	27.0	37.0	18.0	39.0
20	31.775424454333546	34.0	29.0	37.0	19.0	39.0
21	32.641018859927954	34.0	31.0	37.0	23.0	39.0
22	33.128518753973296	35.0	31.0	37.0	25.0	39.0
23	34.408407713498626	35.0	33.0	38.0	28.0	40.0
24	34.05830726848909	35.0	33.0	38.0	27.0	40.0
25	32.86929095147277	35.0	31.0	38.0	23.0	40.0
26	26.599543971180335	33.0	21.0	36.0	0.0	39.0
27	29.850598855689764	34.0	24.0	37.0	15.0	39.0
28	31.273714770078406	34.0	28.0	37.0	19.0	39.0
29	32.574458147912694	35.0	30.0	37.0	23.0	39.0
30	32.390217418944694	35.0	30.0	38.0	22.0	39.0
31	31.182586141131594	34.0	29.0	37.0	19.0	39.0
32	31.576016952744226	34.0	30.0	37.0	19.0	39.0
33	31.381466412375502	34.0	30.0	37.0	18.0	39.0
34	31.428244119516847	34.0	30.0	37.0	18.0	39.0
35	30.717467260012715	34.0	29.0	37.0	15.0	39.0
36	30.500593346047893	34.0	28.0	37.0	13.0	39.0
37	30.601328247510065	34.0	29.0	37.0	12.0	39.0
38	30.328505191777918	34.0	28.0	37.0	12.0	39.0
39	30.194816698453064	34.0	27.0	37.0	10.0	39.0
40	30.087019283746557	34.0	27.0	37.0	11.0	39.0
41	29.458500529773257	33.0	26.0	36.0	10.0	38.0
42	29.625445221445222	33.0	26.0	36.0	10.0	38.0
43	29.162817546090274	33.0	26.0	36.0	10.0	38.0
44	28.99520915448188	33.0	26.0	36.0	10.0	38.0
45	28.51633142614961	32.0	24.0	35.0	10.0	38.0
46	28.103271879635518	31.0	23.0	35.0	9.0	38.0
47	28.147823691460054	32.0	23.0	35.0	10.0	38.0
48	28.203977113795297	32.0	24.0	35.0	10.0	38.0
49	28.043545666454758	31.0	24.0	35.0	9.0	38.0
50	29.966650561559653	34.0	27.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	9.0
11	25.0
12	63.0
13	183.0
14	402.0
15	907.0
16	1838.0
17	3335.0
18	5437.0
19	8550.0
20	12100.0
21	17007.0
22	22750.0
23	29453.0
24	37889.0
25	46917.0
26	55583.0
27	62681.0
28	71405.0
29	84045.0
30	100359.0
31	117431.0
32	128188.0
33	128198.0
34	111790.0
35	84050.0
36	40961.0
37	7987.0
38	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.48061029879212	23.08353464717101	27.921847849120578	22.514007204916293
2	35.360796778978596	17.904979868616234	32.24700148336512	14.48722186904005
3	29.264505191777918	15.10947234583598	40.65183301546938	14.97418944691672
4	26.467810976901884	25.326891290527655	33.86217418944691	14.343123543123543
5	20.21360457724094	29.696800169527442	36.73117185844458	13.35842339478703
6	19.44276329730875	35.642975206611574	34.186564950201316	10.727696545878365
7	80.86501377410468	3.0999788090697185	14.366094511549058	1.6689129052765417
8	82.85611358338632	1.8297096842551388	13.544988344988345	1.7691883873702057
9	70.12078830260648	2.8873066327611783	13.803771985590169	13.188133079042169
10	44.670904852723034	21.833100233100232	22.04204280567917	11.453952108497562
11	38.65132443314261	21.33824962915872	27.242636151727062	12.767789785971603
12	34.49518965882602	20.698792116973934	30.95528713710532	13.850731087094722
13	20.851197287560925	32.07128628946811	31.532273786819243	15.545242636151727
14	16.27200678109769	32.73752913752914	34.78626827717737	16.204195804195802
15	14.700063572790844	20.318033481669843	46.02085187539733	18.96105107014198
16	16.795168467895742	16.91256622165713	43.968891714346256	22.32337359610087
17	18.008815426997245	18.398813307904216	34.751430387794024	28.840940877304515
18	21.852256834075014	21.282559864378044	37.79478703115067	19.07039627039627
19	26.443399025217207	22.60631489722399	31.884212756940027	19.066073320618777
20	27.85912269548633	22.409832591650776	30.94536978173342	18.785674931129474
21	21.5659249841068	25.297647806738716	33.55660097478279	19.579826234371687
22	21.39571943208307	21.38537825810553	33.20559440559441	24.013307904216997
23	19.138885357067174	25.969824115278662	32.72210214028396	22.169188387370205
24	19.132443314261497	21.4974358974359	41.14431023521933	18.22581055308328
25	19.102691248145792	21.155244755244755	38.89374867556686	20.848315321042595
26	14.17062937062937	40.160033905488454	27.18177579995762	18.48756092392456
27	17.28951048951049	28.498410680228865	34.86620046620047	19.34587836406018
28	16.130451366815002	24.650137741046834	40.61970756516211	18.599703326976055
29	16.809663064208518	23.593388429752064	39.14388641661369	20.453062089425725
30	19.907522780250055	24.57868192413647	36.42068234795507	19.0931129476584
31	26.63860987497351	22.74583598219962	31.72985802076711	18.88569612205976
32	26.83738080101716	23.016656071201524	31.80682347955075	18.339139648230557
33	25.041152786607334	24.014918414918416	30.47086247086247	20.473066327611782
34	18.85619834710744	24.97995337995338	32.92570459843187	23.23814367450731
35	20.25081585081585	24.74575121847849	32.94714981987709	22.056283110828563
36	26.22962492053401	24.592583174401355	28.975799957618136	20.201991947446494
37	19.635092180546724	29.95583810129265	29.81114642932825	20.59792328883238
38	21.342996397541853	29.746217418944692	27.91311718584446	20.997668997669
39	20.653697817334184	28.191142191142195	28.544776435685527	22.6103835558381
40	24.071031998304726	24.088323797414706	28.76728120364484	23.073363000635727
41	19.069972451790633	22.701843610934517	29.946175037084128	28.282008900190718
42	21.639330366603094	23.81106166560712	27.122865013774106	27.42674295401568
43	21.21152786607332	24.129688493324856	28.72201737656283	25.93676626403899
44	20.004068658614113	26.846874337783426	30.29921593557957	22.849841068022887
45	18.92350074168256	31.393176520449245	26.490188599279506	23.193134138588682
46	20.676499258317442	28.217842763297305	28.846535282898923	22.259122695486333
47	20.597753761390123	25.207120152574696	29.67501589319771	24.520110192837468
48	20.89747827929646	24.18054672600127	32.66759906759907	22.2543759271032
49	20.856198347107437	23.694426785335875	32.10197075651621	23.347404111040472
50	18.870777707141343	28.59368510277601	30.1168044077135	22.418732782369148
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	309.0
1	776.0
2	1243.0
3	11082.5
4	20922.0
5	16539.5
6	12157.0
7	11933.5
8	11710.0
9	12162.5
10	12615.0
11	12966.5
12	13318.0
13	13122.5
14	12927.0
15	12474.5
16	12022.0
17	11540.5
18	11059.0
19	10517.0
20	9975.0
21	9427.5
22	8880.0
23	8615.0
24	8350.0
25	8371.5
26	8393.0
27	9474.0
28	10555.0
29	12598.0
30	14641.0
31	16826.5
32	19012.0
33	21353.5
34	23695.0
35	25251.5
36	26808.0
37	29799.0
38	32790.0
39	39629.0
40	46468.0
41	57666.5
42	68865.0
43	80602.0
44	92339.0
45	95792.5
46	99246.0
47	97150.0
48	95054.0
49	93214.0
50	91374.0
51	86778.0
52	82182.0
53	74201.5
54	66221.0
55	61657.5
56	57094.0
57	54572.5
58	52051.0
59	47889.0
60	43727.0
61	38905.5
62	34084.0
63	29464.0
64	24844.0
65	21273.0
66	17702.0
67	15098.5
68	12495.0
69	10761.5
70	9028.0
71	7646.5
72	6265.0
73	5149.5
74	4034.0
75	3286.5
76	2539.0
77	1873.0
78	1207.0
79	985.0
80	763.0
81	569.0
82	375.0
83	293.5
84	212.0
85	155.5
86	99.0
87	75.0
88	51.0
89	41.0
90	31.0
91	23.0
92	15.0
93	9.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1179750.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.51970021453524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.52370176776158	34.66363359544399
2	7.278331012138774	6.189449053965911
3	2.8433773892630017	3.6269866256475214
4	1.567189400242847	2.6654569391089256
5	1.060438477813176	2.254476308628716
6	0.7293220726627393	1.86063335336779
7	0.5553238007851412	1.6528541071966347
8	0.4568853305049309	1.5541301828390812
9	0.36622103778854004	1.4014447865122233
>10	3.1709348426564983	28.717109642828525
>50	0.3976519502492004	11.33047698302167
>100	0.05040658087937067	2.985128141325362
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.1633725418494288E-4	1.0982202801136982
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11939	1.011994066539521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02941301123119305	0.0
2	0.0	0.0	0.0	0.11934731934731935	0.0
3	0.0	0.0	0.0	0.16266158084339902	0.0
4	0.0	0.0	0.0	0.23208306844670482	0.0
5	0.0	0.0	0.0	0.3882178427632973	0.0
6	0.0	0.0	0.0	0.5452850180122908	0.0
7	0.0	0.0	0.0	0.6625980080525535	0.0
8	0.0	0.0	0.0	0.9888535706717525	0.0
9	0.0	0.0	0.0	1.1635515999152364	0.0
10	0.0	0.0	0.0	1.4135198135198135	0.0
11	0.0	0.0	0.0	1.5995761813943632	0.0
12	0.0	0.0	0.0	1.7666454757363848	0.0
13	0.0	0.0	0.0	1.834710743801653	0.0
14	0.0	0.0	0.0	1.8559016740834922	0.0
15	0.0	0.0	0.0	1.8856537401991948	0.0
16	0.0	0.0	0.0	1.9460902733630006	0.0
17	0.0	0.0	0.0	2.0311930493748678	0.0
18	0.0	0.0	0.0	2.147743165924984	0.0
19	0.0	0.0	0.0	2.1936003390548846	0.0
20	0.0	0.0	0.0	2.2537825810553085	0.0
21	0.0	0.0	0.0	2.3196439923712653	0.0
22	0.0	0.0	0.0	2.3910998092816276	0.0
23	0.0	0.0	0.0	2.4641661368934096	0.0
24	0.0	0.0	0.0	2.5234159779614327	0.0
25	0.0	0.0	0.0	2.571900826446281	0.0
26	0.0	0.0	0.0	2.6170798898071626	0.0
27	0.0	0.0	0.0	2.6777707141343505	0.0
28	0.0	0.0	0.0	2.7474464929010383	0.0
29	0.0	0.0	0.0	2.8291587200678108	0.0
30	0.0	0.0	0.0	2.9339266793812246	0.0
31	0.0	0.0	0.0	3.021402839584658	0.0
32	0.0	0.0	0.0	3.1037931765204494	0.0
33	0.0	0.0	0.0	3.190760754397118	0.0
34	0.0	0.0	0.0	3.2748463657554567	0.0
35	0.0	0.0	0.0	3.3791057427421065	0.0
36	0.0	0.0	0.0	3.472684890866709	0.0
37	0.0	0.0	0.0	3.5636363636363635	0.0
38	0.0	0.0	0.0	3.682983682983683	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAG	65	0.0	44.000004	1
GGTACGA	20	7.8565674E-4	44.0	38
TAAGTCG	25	4.442912E-5	44.0	18
CGTTTTT	12435	0.0	43.168476	1
ACGGTCT	150	0.0	42.533333	30
CGATAGG	145	0.0	40.96552	2
CGGTATA	55	7.8216544E-11	40.0	43
CGGTCTA	160	0.0	39.875	31
ATACCGG	45	2.3510438E-8	39.11111	2
GTTTTTT	14080	0.0	39.109375	2
CGTAGGC	85	0.0	38.82353	3
CGGAATT	420	0.0	38.761906	9
CGACGGT	165	0.0	38.666668	28
TAGGGTA	560	0.0	38.5	5
GGCGGGA	1850	0.0	37.816216	7
CTATTCG	35	7.2903695E-6	37.714287	44
CTCGTCC	310	0.0	37.6129	37
GACGGTC	170	0.0	37.52941	29
CGGGAAT	1620	0.0	37.48148	9
ATCGTAG	60	1.9826984E-10	36.666664	1
>>END_MODULE