FastQCFastQC Report
Thu 26 May 2016
SRR1546033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546033_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences902565
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163681.8134981968057702No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13320.14757939871366607No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGC207.85558E-444.00000439
CGTTGTC207.85558E-444.00000433
TCTAGCG408.309144E-944.0000041
CTAACGC207.85558E-444.00000420
GTAGATC207.85558E-444.00000441
CGTAGGA207.85558E-444.00000442
GTATGCG408.309144E-944.0000041
TACGACT207.85558E-444.00000436
ACTACGG207.85558E-444.0000042
ACGTCCG207.85558E-444.00000422
GCTACGA408.309144E-944.00000410
GGATTAC408.309144E-944.0000048
CGATAGA408.309144E-944.00000410
AAATCGT454.802132E-1044.012
GTTACGT254.4420773E-544.025
CGGGCTA600.044.06
TCCACCG254.4420773E-544.031
CGCACGG254.4420773E-544.02
CACGTCG502.7284841E-1144.024
TGTCGTA254.4420773E-544.017
Produced by FastQC (version 0.11.5)