##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546033_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	902565
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.263989851146455	31.0	28.0	33.0	25.0	33.0
2	28.845729670439248	31.0	28.0	33.0	16.0	33.0
3	28.18074044528649	31.0	27.0	33.0	16.0	34.0
4	31.916475821685975	35.0	32.0	35.0	25.0	37.0
5	33.63949854027134	35.0	33.0	35.0	28.0	37.0
6	33.5333477367281	35.0	33.0	37.0	28.0	37.0
7	34.214797826195344	35.0	33.0	37.0	30.0	37.0
8	34.7709605402381	35.0	35.0	37.0	32.0	37.0
9	36.65710059663293	39.0	37.0	39.0	32.0	39.0
10	36.5144349714425	39.0	35.0	39.0	32.0	39.0
11	36.432290195166	39.0	35.0	39.0	32.0	39.0
12	35.13066427348723	37.0	34.0	39.0	30.0	39.0
13	34.80782547517353	37.0	33.0	39.0	26.0	39.0
14	35.657811902743845	38.0	33.0	40.0	27.0	41.0
15	36.39669497487716	38.0	35.0	40.0	31.0	41.0
16	36.09001456958779	37.0	34.0	40.0	31.0	41.0
17	35.6570341194263	37.0	34.0	40.0	30.0	41.0
18	35.28548968772332	36.0	34.0	39.0	29.0	40.0
19	34.68349426357104	36.0	33.0	39.0	27.0	40.0
20	34.951446156232514	35.0	34.0	39.0	29.0	40.0
21	35.0976827153723	35.0	34.0	39.0	30.0	40.0
22	35.42479489011872	35.0	34.0	39.0	31.0	40.0
23	35.76352617263023	35.0	34.0	39.0	31.0	41.0
24	35.43413936946369	35.0	34.0	39.0	31.0	41.0
25	35.0640264136101	35.0	34.0	39.0	30.0	41.0
26	35.11029344146959	35.0	34.0	39.0	30.0	41.0
27	35.38747015450411	35.0	34.0	39.0	31.0	41.0
28	35.36424966622903	35.0	34.0	40.0	31.0	41.0
29	35.51700874729244	36.0	35.0	40.0	31.0	41.0
30	35.113273836233404	35.0	34.0	40.0	30.0	41.0
31	34.35778586583792	35.0	34.0	39.0	27.0	40.0
32	34.06723504678333	35.0	33.0	39.0	24.0	40.0
33	33.56310404236814	35.0	33.0	39.0	21.0	41.0
34	33.07428163068588	35.0	33.0	40.0	18.0	41.0
35	32.66379485134035	35.0	33.0	40.0	15.0	41.0
36	32.17909181056212	35.0	32.0	39.0	10.0	41.0
37	32.02803122212805	35.0	31.0	39.0	10.0	41.0
38	31.918486757186464	35.0	31.0	39.0	10.0	40.0
39	31.77340911734889	35.0	31.0	39.0	10.0	40.0
40	31.577864198146393	35.0	31.0	39.0	10.0	40.0
41	31.347141757103365	35.0	30.0	38.0	10.0	40.0
42	31.482501537285405	35.0	31.0	39.0	10.0	40.0
43	31.251157534360406	35.0	30.0	38.0	9.0	40.0
44	31.018785350639565	35.0	30.0	38.0	8.0	40.0
45	30.948056926647943	35.0	30.0	38.0	8.0	40.0
46	30.8052771822528	35.0	29.0	38.0	8.0	40.0
47	30.78715549572607	35.0	30.0	38.0	8.0	40.0
48	30.835499936292678	35.0	30.0	38.0	8.0	40.0
49	30.847333987025866	35.0	30.0	38.0	8.0	40.0
50	30.89840953283143	35.0	29.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	5.0
13	15.0
14	43.0
15	112.0
16	281.0
17	599.0
18	1285.0
19	2262.0
20	3439.0
21	5136.0
22	7412.0
23	10429.0
24	15610.0
25	23825.0
26	33158.0
27	35990.0
28	32181.0
29	28857.0
30	30139.0
31	35408.0
32	48650.0
33	76539.0
34	127212.0
35	76871.0
36	69813.0
37	98392.0
38	113121.0
39	25777.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.509592106939667	21.57650695517774	26.966811254591082	26.94708968329151
2	39.60501459728662	20.406286527840102	28.95403655138411	11.034662323489167
3	25.117082980173173	18.720202977070905	44.360239982715925	11.802474060039996
4	22.589397993496313	24.02187100098054	40.768033327239586	12.620697678283557
5	18.94799820511542	27.84032174968008	41.45053264861811	11.761147396586395
6	17.37559067767972	34.043309900118	38.7398137530261	9.84128566917618
7	72.7646208306327	4.312708779977066	20.701445325267432	2.2212250641228057
8	74.2919346529059	3.0275935805177467	20.30180651809011	2.3786652484862585
9	71.22146327411322	4.591359071091833	21.19293347293547	2.9942441818594783
10	46.40386010979818	21.21354140699008	24.5659869372289	7.816611545982838
11	42.24150061214428	15.948879028103239	32.42669503027483	9.382925329477656
12	37.99150199708608	18.479777079767107	33.099222770659175	10.429498152487632
13	17.730911347105195	38.1523768371253	33.80100048195975	10.315711333809753
14	12.134084525768227	38.555228709289636	37.24241467373541	12.068272091206728
15	10.94957149900561	19.033310620287736	58.664029737470436	11.35308814323622
16	12.642967542503863	15.729393450887194	55.70457529374616	15.923063712862787
17	13.39648667962972	15.809166098840526	38.708901851944184	32.08544536958557
18	18.30682554719051	20.0233778176641	44.44411205841131	17.225684576734086
19	24.793671369929037	20.56450227961421	36.967199038296414	17.674627312160343
20	26.31832610393711	20.34014170724546	35.72318891160193	17.61834327721549
21	17.66011312204661	25.14666533712254	38.791665974195766	18.40155556663509
22	18.97536465517719	20.891237750189738	35.92838188939301	24.205015705240065
23	15.637544110396481	26.39676920775789	35.70169461479228	22.263992067053344
24	16.021339183327516	19.415554558397456	50.0853678128445	14.477738445430525
25	13.22630503066261	21.850947023206086	45.99668721920305	18.926060726928252
26	13.429392896910471	30.612642856747158	38.772276788929325	17.18568745741304
27	13.997883808922348	30.518356018680095	39.48679596483356	15.996964207563998
28	11.868508085290257	24.20390775179627	49.415609956069645	14.511974206843828
29	12.724956097344789	20.67817830294771	47.00160099272628	19.595264606981214
30	15.823015516887981	26.302593165035205	41.433359370239266	16.44103194783755
31	25.76567892617152	22.288810224194382	35.69560087085141	16.249909978782693
32	26.82399605568574	21.97426224150061	36.264645759585186	14.937095943228465
33	24.72121121470476	23.46955620924809	34.291159085495224	17.518073490551927
34	17.372377612692716	24.180529934132167	36.08637605047836	22.36071640269676
35	18.433243035127663	24.032396558696604	37.69534604155933	19.839014364616396
36	27.900151235645076	22.92455391024469	31.84003368178469	17.335261172325538
37	18.352694819763673	30.832017638618826	33.81739819292793	16.99788934868957
38	19.550724878540603	30.42373679457989	30.003933234725476	20.021605092154026
39	19.687224742816305	29.096851750289453	31.931439840897884	19.284483665996355
40	22.71570468608909	24.43103820777451	30.569654263127866	22.283602843008538
41	16.11617999811648	23.303363192678646	32.305263332834755	28.275193476370124
42	21.46659797355315	23.299374560280977	28.544536958557003	26.68949050760887
43	21.803415820467222	24.32954967232277	29.52529734700548	24.341737160204527
44	18.500828195199237	29.239334563161655	31.121193487449656	21.13864375418945
45	16.374000764487874	35.60840493482464	26.24752787887853	21.770066421808956
46	20.723936780176498	30.035177521840534	29.5240785982173	19.716807099765667
47	20.046312453950684	24.99631605479938	31.38499720241755	23.572374288832386
48	21.680876169583353	21.85227656733864	35.180956496208026	21.285890766869976
49	20.223917390991232	22.32359996232958	34.57102812539817	22.881454521281018
50	18.070499077628757	30.952008996581963	30.214222798358016	20.763269127431265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	439.0
1	594.0
2	749.0
3	12870.0
4	24991.0
5	18758.5
6	12526.0
7	13389.5
8	14253.0
9	15247.5
10	16242.0
11	16671.5
12	17101.0
13	16353.5
14	15606.0
15	14734.5
16	13863.0
17	12966.5
18	12070.0
19	11016.5
20	9963.0
21	9220.5
22	8478.0
23	7851.5
24	7225.0
25	6816.5
26	6408.0
27	7384.5
28	8361.0
29	8909.0
30	9457.0
31	11027.0
32	12597.0
33	14500.5
34	16404.0
35	19504.5
36	22605.0
37	23060.0
38	23515.0
39	32303.5
40	41092.0
41	57167.5
42	73243.0
43	83419.5
44	93596.0
45	93934.5
46	94273.0
47	86869.0
48	79465.0
49	73433.5
50	67402.0
51	61057.5
52	54713.0
53	48061.5
54	41410.0
55	35236.0
56	29062.0
57	26119.0
58	23176.0
59	20234.5
60	17293.0
61	14910.0
62	12527.0
63	10491.0
64	8455.0
65	6826.0
66	5197.0
67	4183.0
68	3169.0
69	2593.5
70	2018.0
71	1740.0
72	1462.0
73	1150.5
74	839.0
75	722.0
76	605.0
77	484.0
78	363.0
79	258.5
80	154.0
81	139.0
82	124.0
83	80.5
84	37.0
85	27.0
86	17.0
87	11.5
88	6.0
89	4.5
90	3.0
91	5.0
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	902565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.104359403403784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.82740088539398	23.233253656081
2	6.765130493356933	3.9378957857917305
3	2.8378747374300666	2.477835789000144
4	1.714182198061796	1.995606991012288
5	1.210024519266588	1.7608494247832833
6	0.9008983876754112	1.5732042276511295
7	0.6599778719313807	1.344576322808915
8	0.5515735171325362	1.2842555104019864
9	0.4382381314276198	1.1479176073210995
>10	3.7450731871986087	23.877628543587313
>50	0.9466842762299776	19.47974937176141
>100	0.40179707388689184	15.854483881471182
>500	3.815736694082543E-4	0.06707696156853382
>1k	3.815736694082543E-4	0.14792469008160108
>5k	0.0	0.0
>10k+	3.815736694082543E-4	1.8177412366784131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16368	1.8134981968057702	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1332	0.14757939871366607	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1079534437962917E-4	0.0	0.0	0.04830677014951832	0.0
2	1.1079534437962917E-4	0.0	0.0	0.1750566441198141	0.0
3	1.1079534437962917E-4	0.0	0.0	0.2441929390127027	0.0
4	1.1079534437962917E-4	0.0	0.0	0.36307634353204477	0.0
5	1.1079534437962917E-4	0.0	0.0	0.6179056356051918	0.0
6	1.1079534437962917E-4	0.0	0.0	0.8433741614177372	0.0
7	1.1079534437962917E-4	0.0	0.0	1.00092514112557	0.0
8	1.1079534437962917E-4	0.0	0.0	1.4279303983646607	0.0
9	1.1079534437962917E-4	0.0	0.0	1.62237622775091	0.0
10	1.1079534437962917E-4	0.0	0.0	1.9834582550841213	0.0
11	1.1079534437962917E-4	0.0	0.0	2.2798358012996296	0.0
12	1.1079534437962917E-4	0.0	0.0	2.5451906510888413	0.0
13	4.431813775185167E-4	0.0	0.0	2.648341116706276	0.0
14	5.539767218981458E-4	0.0	0.0	2.6846819896627943	0.0
15	5.539767218981458E-4	0.0	0.0	2.741630796673924	0.0
16	6.64772066277775E-4	0.0	0.0	2.875360777340136	0.0
17	6.64772066277775E-4	0.0	0.0	3.0374543661675335	0.0
18	6.64772066277775E-4	0.0	0.0	3.2917296815187824	0.0
19	8.863627550370334E-4	0.0	0.0	3.3904483333610322	0.0
20	9.971580994166626E-4	0.0	0.0	3.5037919706613927	0.0
21	9.971580994166626E-4	0.0	0.0	3.6499310298981236	0.0
22	9.971580994166626E-4	0.0	0.0	3.793189410180984	0.0
23	9.971580994166626E-4	0.0	0.0	3.9600471988167056	0.0
24	0.0011079534437962917	0.0	0.0	4.07516356162714	0.0
25	0.0011079534437962917	0.0	0.0	4.171112329859899	0.0
26	0.0011079534437962917	0.0	0.0	4.2671718934370375	0.0
27	0.0011079534437962917	0.0	0.0	4.359575210649648	0.0
28	0.0011079534437962917	0.0	0.0	4.4545268207829904	0.0
29	0.0011079534437962917	0.0	0.0	4.561111942076194	0.0
30	0.0011079534437962917	0.0	0.0	4.708802136134239	0.0
31	0.0011079534437962917	0.0	0.0	4.844526432999285	0.0
32	0.0011079534437962917	0.0	0.0	4.9516655310143864	0.0
33	0.0011079534437962917	0.0	0.0	5.06434439624847	0.0
34	0.0012187487881759208	0.0	0.0	5.178352805615108	0.0
35	0.0012187487881759208	0.0	0.0	5.331471971547756	0.0
36	0.0012187487881759208	0.0	0.0	5.444040041437459	0.0
37	0.0012187487881759208	0.0	0.0	5.569681961963958	0.0
38	0.0012187487881759208	0.0	0.0	5.683136394608699	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGC	20	7.85558E-4	44.000004	39
CGTTGTC	20	7.85558E-4	44.000004	33
TCTAGCG	40	8.309144E-9	44.000004	1
CTAACGC	20	7.85558E-4	44.000004	20
GTAGATC	20	7.85558E-4	44.000004	41
CGTAGGA	20	7.85558E-4	44.000004	42
GTATGCG	40	8.309144E-9	44.000004	1
TACGACT	20	7.85558E-4	44.000004	36
ACTACGG	20	7.85558E-4	44.000004	2
ACGTCCG	20	7.85558E-4	44.000004	22
GCTACGA	40	8.309144E-9	44.000004	10
GGATTAC	40	8.309144E-9	44.000004	8
CGATAGA	40	8.309144E-9	44.000004	10
AAATCGT	45	4.802132E-10	44.0	12
GTTACGT	25	4.4420773E-5	44.0	25
CGGGCTA	60	0.0	44.0	6
TCCACCG	25	4.4420773E-5	44.0	31
CGCACGG	25	4.4420773E-5	44.0	2
CACGTCG	50	2.7284841E-11	44.0	24
TGTCGTA	25	4.4420773E-5	44.0	17
>>END_MODULE