FastQCFastQC Report
Thu 26 May 2016
SRR1546032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546032_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences908524
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171031.8825039294504051No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13240.14573087777538074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG351.4456418E-744.01
ACGCCCA207.8556105E-444.035
GGTCGTT207.8556105E-444.025
ACGCATA254.4421016E-544.032
ACGTTAG600.044.01
TTTCGCG207.8556105E-444.01
CTATACG351.4456418E-744.01
ACCGACG551.8189894E-1244.039
ATCGTAG302.5271293E-644.01
TATGACA551.8189894E-1244.031
ACGATAT207.8556105E-444.034
GTAGATC207.8556105E-444.041
GTAGACG351.4456418E-744.01
CTACGCG207.8556105E-444.01
CTACGAT207.8556105E-444.032
TGTTACG207.8556105E-444.01
TATAGCG254.4421016E-544.01
TTACGCC254.4421016E-544.014
CGGTCTA600.044.031
GTCCCCG207.8556105E-444.042
Produced by FastQC (version 0.11.5)