FastQCFastQC Report
Thu 26 May 2016
SRR1546031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546031_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences892213
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171401.921065933807286No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA14400.1613964378461197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCAA650.044.00000415
CGGACAA207.855533E-444.06
TCACGAC351.4456054E-744.025
CTATGCG302.5270783E-644.01
GTACCGG454.802132E-1044.02
TGCCCTA408.309144E-944.024
CGAAACG207.855533E-444.015
ACGCATA207.855533E-444.032
CCGATCA254.442036E-544.011
CTATACG254.442036E-544.01
ATTCGGC207.855533E-444.012
AAGCGAC351.4456054E-744.015
TACGGCA254.442036E-544.040
TCGATAT254.442036E-544.016
ATCCGAT207.855533E-444.025
CCCGTCG207.855533E-444.044
TAACATG600.044.01
AAGCACG254.442036E-544.01
TATTCGA207.855533E-444.013
ACGGAAT254.442036E-544.05
Produced by FastQC (version 0.11.5)