##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546031_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	892213
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.11591962905719	31.0	28.0	33.0	25.0	33.0
2	28.67749853454276	31.0	28.0	33.0	16.0	33.0
3	28.044518517439222	31.0	26.0	33.0	16.0	34.0
4	31.779496600027123	33.0	30.0	35.0	25.0	37.0
5	33.603405240676835	35.0	33.0	35.0	28.0	37.0
6	33.548724351696286	35.0	33.0	37.0	28.0	37.0
7	34.16599623632474	35.0	33.0	37.0	30.0	37.0
8	34.75487131436103	35.0	35.0	37.0	32.0	37.0
9	36.7401663055795	39.0	37.0	39.0	32.0	39.0
10	36.57399298149657	39.0	35.0	39.0	32.0	39.0
11	36.47432283546642	39.0	35.0	39.0	32.0	39.0
12	35.23054472418582	37.0	34.0	39.0	30.0	39.0
13	34.89895238020517	37.0	33.0	39.0	27.0	39.0
14	35.72877889024257	38.0	33.0	40.0	27.0	41.0
15	36.40294526082897	38.0	35.0	40.0	31.0	41.0
16	36.076133165510925	37.0	34.0	40.0	31.0	41.0
17	35.65205057536709	37.0	34.0	40.0	30.0	41.0
18	35.28897023468611	36.0	34.0	39.0	29.0	40.0
19	34.66072899632711	36.0	33.0	39.0	27.0	40.0
20	34.99879961399352	35.0	34.0	39.0	30.0	40.0
21	35.06819671984156	35.0	34.0	39.0	30.0	40.0
22	35.40343729580268	35.0	34.0	39.0	31.0	40.0
23	35.76118034594878	35.0	34.0	39.0	31.0	41.0
24	35.35869237502704	35.0	34.0	39.0	31.0	41.0
25	35.00447987195883	35.0	34.0	39.0	30.0	41.0
26	35.074706376168024	35.0	34.0	39.0	30.0	41.0
27	35.39401129550903	35.0	34.0	39.0	31.0	41.0
28	35.381903200244786	35.0	34.0	40.0	31.0	41.0
29	35.51401290947341	36.0	35.0	40.0	31.0	41.0
30	35.14955285341056	35.0	34.0	40.0	30.0	41.0
31	34.37790975921669	35.0	34.0	39.0	27.0	40.0
32	34.126792593248474	35.0	34.0	39.0	24.0	40.0
33	33.63588403217617	35.0	33.0	40.0	21.0	41.0
34	33.073954313600005	35.0	33.0	40.0	17.0	41.0
35	32.65628162781757	35.0	33.0	40.0	15.0	41.0
36	32.203054651747955	35.0	32.0	39.0	10.0	41.0
37	32.07502468580933	35.0	31.0	39.0	10.0	41.0
38	31.98260953382208	35.0	31.0	39.0	10.0	41.0
39	31.775883113113125	35.0	31.0	39.0	10.0	40.0
40	31.600689521448352	35.0	31.0	39.0	10.0	40.0
41	31.364516096492654	35.0	31.0	39.0	10.0	40.0
42	31.533351340991445	35.0	31.0	39.0	10.0	40.0
43	31.3043297956878	35.0	30.0	39.0	9.0	40.0
44	31.075497667036906	35.0	30.0	38.0	8.0	40.0
45	31.018347636719035	35.0	30.0	38.0	8.0	40.0
46	30.85874785505255	35.0	29.0	38.0	8.0	40.0
47	30.850124353713742	35.0	30.0	38.0	8.0	40.0
48	30.88532558929314	35.0	30.0	38.0	8.0	40.0
49	30.959229466506315	35.0	30.0	38.0	8.0	40.0
50	30.959871689831914	35.0	30.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	10.0
14	31.0
15	110.0
16	249.0
17	649.0
18	1231.0
19	2172.0
20	3418.0
21	4973.0
22	7269.0
23	10523.0
24	15329.0
25	23571.0
26	33137.0
27	35768.0
28	30907.0
29	28453.0
30	29454.0
31	34900.0
32	47513.0
33	76316.0
34	126437.0
35	75488.0
36	68181.0
37	97929.0
38	112783.0
39	25408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.622147402021717	21.443197980751233	26.851884023209706	27.082770594017347
2	39.583260947778165	20.43121989928414	28.87752139903812	11.107997753899573
3	25.05141709434855	18.689819583440276	44.412376865165605	11.846386457045572
4	22.47445397007217	23.99987446943723	40.65352107624525	12.872150484245354
5	18.80167628133641	27.767472565407587	41.602958038046964	11.827893115209037
6	17.404476285371317	34.015644246385115	38.831422541478325	9.748456926765245
7	72.44783476591353	4.381352883224073	20.895682981530193	2.275129369332211
8	73.98334254264397	3.114278765272418	20.45968843762644	2.4426902544571756
9	71.0488414761946	4.685988659658625	21.358352770022403	2.906817094124385
10	46.33064077748251	21.233270530691662	24.689732160369775	7.746356531456054
11	42.13321258488724	15.991921211638923	32.512864080662354	9.362002122811482
12	37.89924603205736	18.5046620033557	33.18859958328336	10.407492381303568
13	17.72626043332702	38.05985790388618	33.807958413517845	10.405923249268954
14	12.140934956114739	38.4999994395957	37.32606451598441	12.033001088305147
15	10.911968330432307	18.99411911729598	58.66693267190682	11.42697988036489
16	12.59418995239926	15.74052384352167	55.72010271089975	15.945183493179318
17	13.361047193887558	15.885332314144717	38.66868113331682	32.08493935865091
18	18.31188292481728	20.07491484656691	44.33739477008293	17.275807458532885
19	24.686593896300547	20.6075230914591	37.04743149898063	17.658451513259728
20	26.216721791769455	20.31398332012647	35.801316501777045	17.667978386327032
21	17.722113441521252	24.91792879054665	38.94182218819945	18.418135579732642
22	18.95332168439599	20.966518084807102	36.06280114725968	24.017359083537226
23	15.665429667579378	26.29798041499059	35.77856408727512	22.258025830154907
24	16.117788016986974	19.420923030711275	50.01496279475865	14.4463261575431
25	13.211307165441436	21.836265555422305	45.95998937473451	18.99243790440175
26	13.45306557963177	30.537999334239696	38.8341124821091	17.174822604019443
27	13.907553465372057	30.532507372118538	39.63190404085123	15.92803512165817
28	11.837419988276341	24.285344418877557	49.35200451013379	14.525231082712311
29	12.71568560422231	20.529851055745656	47.08561744785158	19.668845892180457
30	15.770673594758202	26.19946133938869	41.581550593860435	16.448314471992674
31	25.67750077615995	22.27416547393952	35.8184648732982	16.229868876602335
32	26.67759828650782	21.928059779447285	36.42179614060768	14.972545793437217
33	24.59199765078518	23.445074214341194	34.47506368994848	17.487864444925147
34	17.351125796194406	24.226838210158334	36.162553112317354	22.259482881329905
35	18.41017783869995	24.07642569655452	37.65759969872665	19.855796766018877
36	27.864310428115257	22.972989633641294	31.972298094737468	17.190401843505978
37	18.274896241144212	30.829297488380014	34.04568191676203	16.850124353713746
38	19.580189932224705	30.359230363153195	30.148294185357084	19.91228551926502
39	19.49164605312857	29.026252699747708	32.22761829294126	19.254482954182464
40	22.649972596229826	24.516230989685198	30.64324326141852	22.19055315266646
41	16.18884728198311	23.285134827670074	32.47408410323544	28.051933787111373
42	21.585988995901204	23.298920773402763	28.64887644542279	26.466213785273247
43	21.834136019089613	24.475993961083283	29.494750692939913	24.19511932688719
44	18.354809894049964	29.3917483829534	31.209699925914553	21.043741797082085
45	16.353830307336924	35.62546163304054	26.42911502074056	21.59159303888197
46	20.74213220385715	30.028815989007107	29.6661223272918	19.562929479843937
47	20.067069186393834	25.093783659283154	31.40303940875105	23.436107745571967
48	21.867872357833836	21.941509482601127	35.058220402527205	21.13239775703784
49	20.28215235599571	22.316980362312584	34.635339319198444	22.765527962493262
50	18.074607745011562	30.84252302981463	30.4161674398378	20.666701785336013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	409.0
1	604.0
2	799.0
3	13402.0
4	26005.0
5	19401.5
6	12798.0
7	13431.5
8	14065.0
9	15236.5
10	16408.0
11	16652.0
12	16896.0
13	16197.5
14	15499.0
15	14605.0
16	13711.0
17	12663.0
18	11615.0
19	10663.5
20	9712.0
21	8978.5
22	8245.0
23	7554.0
24	6863.0
25	6538.5
26	6214.0
27	7056.0
28	7898.0
29	8532.5
30	9167.0
31	10698.0
32	12229.0
33	14251.0
34	16273.0
35	19313.0
36	22353.0
37	23009.0
38	23665.0
39	32159.5
40	40654.0
41	56569.5
42	72485.0
43	82475.5
44	92466.0
45	92817.5
46	93169.0
47	85642.5
48	78116.0
49	72420.5
50	66725.0
51	60616.5
52	54508.0
53	47581.0
54	40654.0
55	34690.0
56	28726.0
57	25740.0
58	22754.0
59	19744.0
60	16734.0
61	14541.0
62	12348.0
63	10320.0
64	8292.0
65	6739.0
66	5186.0
67	4091.5
68	2997.0
69	2520.5
70	2044.0
71	1733.0
72	1422.0
73	1119.5
74	817.0
75	708.5
76	600.0
77	490.5
78	381.0
79	255.5
80	130.0
81	111.5
82	93.0
83	65.0
84	37.0
85	29.5
86	22.0
87	17.5
88	13.0
89	8.0
90	3.0
91	3.5
92	4.0
93	5.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	892213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.63428669438862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.20632675553232	22.680166683273363
2	6.984851493698025	4.000124803765556
3	2.870913964059934	2.4661972056544754
4	1.7534516212940543	2.00835345715498
5	1.2628973901803913	1.8081082968010247
6	0.9317062897870327	1.600724701003621
7	0.7220737660387297	1.4473247061874916
8	0.5417403659263023	1.240987916148539
9	0.4812212638669	1.2401484869700767
>10	3.8819955525517686	24.337462120166542
>50	0.9610194036180434	19.53458671470602
>100	0.40062151993001066	15.4760605997528
>500	3.9353783883105167E-4	0.06603443727076563
>1k	3.9353783883105167E-4	0.1622689243513695
>5k	0.0	0.0
>10k+	3.9353783883105167E-4	1.9314509467933842
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17140	1.921065933807286	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1440	0.1613964378461197	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04853101221345127	0.0
2	0.0	0.0	0.0	0.16969042145765642	0.0
3	0.0	0.0	0.0	0.23447315831533502	0.0
4	0.0	0.0	0.0	0.353727192946079	0.0
5	0.0	0.0	0.0	0.5880882704017987	0.0
6	0.0	0.0	0.0	0.8059734614940602	0.0
7	0.0	0.0	0.0	0.9540322770459521	0.0
8	0.0	0.0	0.0	1.3625670103439425	0.0
9	0.0	0.0	0.0	1.5510870162169796	0.0
10	0.0	0.0	0.0	1.8866571099053702	0.0
11	0.0	0.0	0.0	2.175041161695694	0.0
12	0.0	0.0	0.0	2.4292405513033324	0.0
13	0.0	0.0	0.0	2.527311303466773	0.0
14	0.0	0.0	0.0	2.5610476422109967	0.0
15	1.120808596153609E-4	0.0	0.0	2.609914897003294	0.0
16	1.120808596153609E-4	0.0	0.0	2.7308501445282687	0.0
17	1.120808596153609E-4	0.0	0.0	2.8835042753243902	0.0
18	1.120808596153609E-4	0.0	0.0	3.115847897307033	0.0
19	1.120808596153609E-4	0.0	0.0	3.209547495945475	0.0
20	1.120808596153609E-4	0.0	0.0	3.3332847649608333	0.0
21	1.120808596153609E-4	0.0	0.0	3.4768603461281105	0.0
22	1.120808596153609E-4	0.0	0.0	3.61169362024539	0.0
23	1.120808596153609E-4	0.0	0.0	3.7708484408992025	0.0
24	1.120808596153609E-4	0.0	0.0	3.886291726303024	0.0
25	1.120808596153609E-4	0.0	0.0	3.978085950328005	0.0
26	1.120808596153609E-4	0.0	0.0	4.063715727074141	0.0
27	1.120808596153609E-4	0.0	0.0	4.151362959293353	0.0
28	1.120808596153609E-4	0.0	0.0	4.245622962229871	0.0
29	1.120808596153609E-4	0.0	0.0	4.349521919093311	0.0
30	1.120808596153609E-4	0.0	0.0	4.491080044787512	0.0
31	1.120808596153609E-4	0.0	0.0	4.609549513400948	0.0
32	1.120808596153609E-4	0.0	0.0	4.711094772212465	0.0
33	1.120808596153609E-4	0.0	0.0	4.819140720881673	0.0
34	1.120808596153609E-4	0.0	0.0	4.937722270354724	0.0
35	1.120808596153609E-4	0.0	0.0	5.087686460520078	0.0
36	1.120808596153609E-4	0.0	0.0	5.210639163518128	0.0
37	1.120808596153609E-4	0.0	0.0	5.326979095798873	0.0
38	1.120808596153609E-4	0.0	0.0	5.44421567495654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCAA	65	0.0	44.000004	15
CGGACAA	20	7.855533E-4	44.0	6
TCACGAC	35	1.4456054E-7	44.0	25
CTATGCG	30	2.5270783E-6	44.0	1
GTACCGG	45	4.802132E-10	44.0	2
TGCCCTA	40	8.309144E-9	44.0	24
CGAAACG	20	7.855533E-4	44.0	15
ACGCATA	20	7.855533E-4	44.0	32
CCGATCA	25	4.442036E-5	44.0	11
CTATACG	25	4.442036E-5	44.0	1
ATTCGGC	20	7.855533E-4	44.0	12
AAGCGAC	35	1.4456054E-7	44.0	15
TACGGCA	25	4.442036E-5	44.0	40
TCGATAT	25	4.442036E-5	44.0	16
ATCCGAT	20	7.855533E-4	44.0	25
CCCGTCG	20	7.855533E-4	44.0	44
TAACATG	60	0.0	44.0	1
AAGCACG	25	4.442036E-5	44.0	1
TATTCGA	20	7.855533E-4	44.0	13
ACGGAAT	25	4.442036E-5	44.0	5
>>END_MODULE