FastQCFastQC Report
Thu 26 May 2016
SRR1546030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546030_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences786146
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87131.1083183021983194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGG302.5266909E-644.02
AGATCGT207.8549614E-444.022
ACGTTAG750.044.01
TAGACGT207.8549614E-444.025
TAGGTCG207.8549614E-444.041
TAGCGGC207.8549614E-444.03
CGTAGAA254.441552E-544.028
TACGACG302.5266909E-644.025
TGCGAAG750.044.01
ACGTGCG207.8549614E-444.01
GTGTACG1000.044.01
TTAGTCG207.8549614E-444.02
GTATACG207.8549614E-444.01
TTACGAC302.5266909E-644.024
CCGGTCA207.8549614E-444.032
TAGGCCG207.8549614E-444.05
GGTTCGT302.5266909E-644.042
GCTCGAA408.307325E-944.021
CTTAGGT207.8549614E-444.019
ACGACGT254.441552E-544.026
Produced by FastQC (version 0.11.5)