##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546030_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	786146
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.313175924064996	28.0	25.0	30.0	16.0	33.0
2	26.21667858133222	30.0	25.0	31.0	10.0	33.0
3	26.384829281075017	30.0	25.0	31.0	16.0	33.0
4	27.346388584308766	32.0	19.0	35.0	10.0	35.0
5	31.181593495355823	33.0	30.0	35.0	19.0	35.0
6	29.986270998008003	33.0	28.0	35.0	17.0	36.0
7	30.119367140454827	32.0	28.0	35.0	17.0	35.0
8	32.60088838460032	35.0	32.0	35.0	28.0	35.0
9	27.38615982273013	31.0	23.0	34.0	10.0	37.0
10	32.38497683636373	35.0	31.0	37.0	23.0	39.0
11	34.06910166813798	35.0	32.0	39.0	27.0	39.0
12	33.3365112841635	35.0	31.0	39.0	25.0	39.0
13	32.77986913372325	35.0	31.0	38.0	22.0	39.0
14	32.6531064204359	35.0	30.0	39.0	21.0	40.0
15	31.9663548501169	34.0	27.0	38.0	18.0	40.0
16	30.67646340501637	34.0	27.0	38.0	17.0	40.0
17	30.690584191740466	32.0	27.0	38.0	17.0	40.0
18	31.64507864951294	34.0	29.0	38.0	18.0	39.0
19	31.244254883952852	34.0	29.0	37.0	18.0	39.0
20	31.86580355302959	34.0	30.0	36.0	23.0	39.0
21	32.47146458800274	34.0	31.0	36.0	23.0	39.0
22	32.71177618406759	34.0	31.0	37.0	24.0	39.0
23	34.02419525126376	35.0	33.0	38.0	28.0	39.0
24	33.667756370953995	35.0	33.0	38.0	26.0	40.0
25	32.43614672083812	34.0	31.0	37.0	23.0	39.0
26	25.98452959119553	33.0	18.0	35.0	0.0	38.0
27	29.18679100319788	33.0	23.0	36.0	11.0	39.0
28	30.824621635167006	34.0	27.0	36.0	18.0	39.0
29	32.15877839485287	34.0	30.0	36.0	23.0	39.0
30	31.872157334642676	34.0	30.0	37.0	21.0	39.0
31	30.767990932981913	33.0	29.0	35.0	18.0	38.0
32	30.730683613476376	34.0	29.0	36.0	18.0	38.0
33	30.379061904531728	33.0	29.0	36.0	15.0	38.0
34	30.206670262266805	34.0	28.0	37.0	12.0	39.0
35	29.268726165368772	33.0	25.0	36.0	9.0	39.0
36	28.846527235399023	33.0	23.0	36.0	8.0	39.0
37	28.73421094809361	33.0	23.0	36.0	8.0	39.0
38	28.584364736321245	33.0	23.0	36.0	8.0	38.0
39	28.502725956756123	33.0	23.0	36.0	8.0	38.0
40	28.478306828502593	33.0	23.0	36.0	8.0	38.0
41	28.00400434524885	32.0	23.0	35.0	8.0	38.0
42	28.155641572939377	33.0	23.0	35.0	7.0	38.0
43	27.721326572926657	32.0	23.0	35.0	7.0	38.0
44	27.356340934126738	32.0	22.0	35.0	7.0	38.0
45	26.819949475033898	31.0	21.0	34.0	7.0	37.0
46	26.482620276640727	30.0	20.0	34.0	7.0	38.0
47	26.67627896090548	31.0	20.0	34.0	7.0	37.0
48	26.80134097228759	31.0	21.0	34.0	7.0	37.0
49	26.66411073770012	31.0	20.0	34.0	7.0	37.0
50	28.273603122066383	33.0	23.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	6.0
11	11.0
12	44.0
13	108.0
14	289.0
15	648.0
16	1327.0
17	2548.0
18	4379.0
19	6896.0
20	10079.0
21	14166.0
22	18911.0
23	24838.0
24	31495.0
25	39065.0
26	44142.0
27	46378.0
28	49464.0
29	55777.0
30	66152.0
31	76667.0
32	80233.0
33	75840.0
34	61411.0
35	46688.0
36	23501.0
37	4936.0
38	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.82972882899614	21.927733525324815	26.72582446517568	28.516713180503366
2	39.84259921185123	17.612122938996063	30.50349426188011	12.041783587272594
3	26.208617737672135	14.391474357180472	46.451422509304884	12.948485395842502
4	23.573483805807065	23.866304732199872	39.964459527873956	12.595751934119107
5	17.959895490150686	28.021894151976863	42.74702154561621	11.271188812256247
6	17.123282443719106	32.624601537119055	40.44363260768356	9.808483411478274
7	74.86408377069908	2.800497617490899	21.026883047169356	1.3085355646406647
8	76.99663930109675	1.5745166928280498	20.26570128195017	1.1631427241250352
9	65.86155752239405	2.7751842533066378	19.734883851091272	11.628374373208029
10	48.035606617600294	20.580782704484918	24.835844741307593	6.5477659366071945
11	43.89057503313634	15.012733003793189	32.8449168475067	8.251775115563776
12	38.902315854815775	18.23452641112465	33.49416011784071	9.36899761621887
13	16.882360274045787	39.397134883342275	34.119489255176525	9.601015587435413
14	11.17247941222114	38.899899000948935	38.33003029971532	11.597591287114607
15	9.526220320398501	17.015160033886836	59.823493346019696	13.635126299694967
16	10.923797869606917	13.717553736837687	56.31320390868871	19.045444484866678
17	12.374419001050697	14.665850872484246	39.746815477023354	33.21291464944171
18	17.870471897077643	19.341572684972004	46.06803316432317	16.71992225362719
19	25.59728091219697	19.35518338832736	38.14749423135143	16.90004146812424
20	26.750374612349358	19.65652690467165	36.57577600089551	17.01732248208348
21	16.954865890050957	24.836480755483077	39.926044271674726	18.282609082791236
22	18.288714818875885	20.386289569621926	36.54295766944053	24.782037942061653
23	14.789619231033422	26.28061454233692	36.74915346513244	22.18061276149723
24	14.912369966901823	18.32687566940492	53.05795615572681	13.70279820796646
25	12.264388548691974	20.622886842901956	48.297517255064584	18.815207353341492
26	10.378352112711887	42.38207661172353	32.49345541413427	14.74611586143032
27	12.73592945839577	31.68660783111534	40.553408654372085	15.024054056116803
28	10.816438676785229	23.84862353812142	51.56166920648327	13.773268578610079
29	11.67747466755539	20.138116838348093	48.79335390627186	19.39105458782465
30	15.194378652311402	26.37181897510132	42.43880907617669	15.99499329641059
31	26.66832878371193	21.670783798429298	36.53481668799434	15.126070729864427
32	27.38855123602995	20.82984585560443	37.22743612509636	14.554166783269265
33	25.197100792982475	22.731019428961034	34.697371735021235	17.374508043035263
34	16.81964927634307	23.42223963487698	36.39285832402633	23.36525276475362
35	18.04931908322373	23.59383625942255	38.05310972770961	20.30373492964411
36	28.445479592849164	22.208978993723814	31.683936571578307	17.661604841848717
37	17.327188588379258	31.863801380405164	33.31111523813643	17.497894793079148
38	18.831489316233878	31.235928186367417	29.756813619861962	20.175768877536743
39	19.185749211978436	29.193305060383185	31.782391565943225	19.838554161695157
40	22.66054905831741	23.660236139343073	30.58325043948579	23.095964362853717
41	15.205445298964824	22.34546763578267	32.540393260284986	29.908693804967523
42	20.649090626931894	22.278177336016466	27.87243082073813	29.200301216313505
43	21.240965418637252	23.101434084762882	29.769661106206733	25.887939390393132
44	17.37743370824249	29.06826467348304	31.517300857601516	22.037000760672953
45	15.416856410895685	37.02696445698382	25.454940939723663	22.10123819239683
46	19.686673976589592	29.91047464465888	29.078695305960977	21.32415607279055
47	19.34398953883884	24.09539703820919	30.772655460944915	25.787957962007056
48	20.90387790563076	21.073948096155167	36.21413834071534	21.808035657498735
49	19.727251680985468	22.090044342908318	34.27569433667538	23.907009639430843
50	17.274653817484285	31.866599842777294	29.90449611140933	20.954250228329087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	208.0
1	344.0
2	480.0
3	7783.0
4	15086.0
5	12577.5
6	10069.0
7	10622.5
8	11176.0
9	12383.5
10	13591.0
11	14434.5
12	15278.0
13	15435.5
14	15593.0
15	15228.0
16	14863.0
17	14157.5
18	13452.0
19	12582.0
20	11712.0
21	10921.0
22	10130.0
23	9450.0
24	8770.0
25	8140.5
26	7511.0
27	7878.5
28	8246.0
29	8742.5
30	9239.0
31	10500.0
32	11761.0
33	13223.5
34	14686.0
35	16716.0
36	18746.0
37	19561.0
38	20376.0
39	27457.5
40	34539.0
41	47719.5
42	60900.0
43	69668.0
44	78436.0
45	78488.0
46	78540.0
47	73011.5
48	67483.0
49	62512.0
50	57541.0
51	52302.5
52	47064.0
53	41803.5
54	36543.0
55	31450.0
56	26357.0
57	23442.0
58	20527.0
59	17847.0
60	15167.0
61	13179.5
62	11192.0
63	9394.5
64	7597.0
65	6216.5
66	4836.0
67	3866.5
68	2897.0
69	2450.5
70	2004.0
71	1659.0
72	1314.0
73	1089.0
74	864.0
75	736.0
76	608.0
77	493.5
78	379.0
79	288.5
80	198.0
81	146.5
82	95.0
83	73.0
84	51.0
85	34.5
86	18.0
87	12.0
88	6.0
89	6.0
90	6.0
91	6.5
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	786146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.95251573353155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.31000347779883	36.21326750874253
2	6.243870758867103	5.363799140167536
3	2.386923413997698	3.0757309648341282
4	1.2795445853617575	2.19838635738024
5	0.8369087421225713	1.7973667956774921
6	0.6014827276870836	1.5501117794656156
7	0.4711110353097409	1.4164782909467388
8	0.3620157073262115	1.2439588291771724
9	0.29346376162981064	1.1344506154763212
>10	2.6403062318043324	24.628933035081097
>50	0.4645779885466844	13.84521208890925
>100	0.10913998753305582	6.226427126095569
>500	3.2579100756135847E-4	0.08662003351057732
>1k	0.0	0.0
>5k	3.2579100756135847E-4	1.2192574345357998
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8713	1.1083183021983194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03319993996026183	0.0
2	0.0	0.0	0.0	0.12796605210736936	0.0
3	0.0	0.0	0.0	0.1779567663004073	0.0
4	0.0	0.0	0.0	0.2522432219969319	0.0
5	0.0	0.0	0.0	0.4160804736015956	0.0
6	0.0	0.0	0.0	0.5472265965863847	0.0
7	0.0	0.0	0.0	0.631943684760846	0.0
8	0.0	0.0	0.0	0.8820244585611324	0.0
9	0.0	0.0	0.0	1.0246188367046325	0.0
10	0.0	0.0	0.0	1.2523119115278842	0.0
11	0.0	0.0	0.0	1.4540556079913909	0.0
12	0.0	0.0	0.0	1.6412981812538638	0.0
13	0.0	0.0	0.0	1.7120229575676782	0.0
14	0.0	0.0	0.0	1.7328842225235517	0.0
15	0.0	0.0	0.0	1.7660841624838135	0.0
16	0.0	0.0	0.0	1.8374449529731118	0.0
17	0.0	0.0	0.0	1.9462033769808662	0.0
18	0.0	0.0	0.0	2.093249854352754	0.0
19	0.0	0.0	0.0	2.1427317572054045	0.0
20	0.0	0.0	0.0	2.220707095119736	0.0
21	0.0	0.0	0.0	2.295756767826841	0.0
22	0.0	0.0	0.0	2.38136427584698	0.0
23	0.0	0.0	0.0	2.4701518547445387	0.0
24	0.0	0.0	0.0	2.5374421545107397	0.0
25	0.0	0.0	0.0	2.5948106331393914	0.0
26	0.0	0.0	0.0	2.6488718380555265	0.0
27	0.0	0.0	0.0	2.7126004584390175	0.0
28	0.0	0.0	0.0	2.7842156545985097	0.0
29	0.0	0.0	0.0	2.8712223938047132	0.0
30	0.0	0.0	0.0	2.976037529924467	0.0
31	0.0	0.0	0.0	3.068895599545123	0.0
32	0.0	0.0	0.0	3.147634154470035	0.0
33	0.0	0.0	0.0	3.227135926405528	0.0
34	0.0	0.0	0.0	3.3072737125165044	0.0
35	0.0	0.0	0.0	3.4251907406512276	0.0
36	0.0	0.0	0.0	3.5155047535699477	0.0
37	0.0	0.0	0.0	3.6070907948396354	0.0
38	0.0	0.0	0.0	3.696895996417968	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGG	30	2.5266909E-6	44.0	2
AGATCGT	20	7.8549614E-4	44.0	22
ACGTTAG	75	0.0	44.0	1
TAGACGT	20	7.8549614E-4	44.0	25
TAGGTCG	20	7.8549614E-4	44.0	41
TAGCGGC	20	7.8549614E-4	44.0	3
CGTAGAA	25	4.441552E-5	44.0	28
TACGACG	30	2.5266909E-6	44.0	25
TGCGAAG	75	0.0	44.0	1
ACGTGCG	20	7.8549614E-4	44.0	1
GTGTACG	100	0.0	44.0	1
TTAGTCG	20	7.8549614E-4	44.0	2
GTATACG	20	7.8549614E-4	44.0	1
TTACGAC	30	2.5266909E-6	44.0	24
CCGGTCA	20	7.8549614E-4	44.0	32
TAGGCCG	20	7.8549614E-4	44.0	5
GGTTCGT	30	2.5266909E-6	44.0	42
GCTCGAA	40	8.307325E-9	44.0	21
CTTAGGT	20	7.8549614E-4	44.0	19
ACGACGT	25	4.441552E-5	44.0	26
>>END_MODULE