FastQCFastQC Report
Thu 26 May 2016
SRR1546029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546029_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1260048
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304672.4179237616344773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG302.5279114E-644.0000041
TAGCACG302.5279114E-644.0000041
TAATGCG302.5279114E-644.0000041
TACGTAG302.5279114E-644.0000041
CTAGCGT302.5279114E-644.00000418
GGTACGA207.8567717E-444.026
TCGATTG254.443084E-544.01
CGCTATC207.8567717E-444.029
CGAATCC207.8567717E-444.020
CTACCGT207.8567717E-444.019
TATCGTA207.8567717E-444.026
TGCACGG750.044.02
TATCGCG351.4462421E-743.9999961
CGTTTTT187100.043.4355961
ACGGGTA1300.042.307695
GCGATAT750.041.0666669
TTGTACG603.6379788E-1240.3333361
ACCCGCT3250.039.93846534
TACGGGA3750.039.8933334
TCACGAC1600.039.87525
Produced by FastQC (version 0.11.5)