##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546029_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1260048
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.12121522354704	31.0	28.0	33.0	25.0	33.0
2	28.955918345967774	31.0	28.0	33.0	16.0	33.0
3	28.27554347135982	31.0	27.0	33.0	16.0	34.0
4	32.13604561096085	35.0	32.0	35.0	25.0	37.0
5	33.68285493885947	35.0	33.0	35.0	28.0	37.0
6	33.55306543877693	35.0	33.0	37.0	28.0	37.0
7	34.31186113544881	35.0	35.0	37.0	31.0	37.0
8	34.77179282059096	35.0	35.0	37.0	32.0	37.0
9	36.586869706550864	39.0	37.0	39.0	32.0	39.0
10	36.3300501250746	38.0	35.0	39.0	32.0	39.0
11	36.44596634413927	39.0	35.0	39.0	32.0	39.0
12	35.22668263431235	37.0	34.0	39.0	29.0	39.0
13	35.39500003174482	37.0	35.0	39.0	30.0	39.0
14	36.127681643873885	38.0	35.0	40.0	29.0	41.0
15	36.68556277221185	38.0	35.0	40.0	31.0	41.0
16	36.30920488743286	38.0	34.0	40.0	31.0	41.0
17	35.91243666907927	37.0	34.0	40.0	30.0	41.0
18	35.53156625779335	37.0	34.0	39.0	29.0	40.0
19	34.96165225451729	37.0	33.0	39.0	27.0	40.0
20	35.29633077470065	35.0	34.0	39.0	30.0	40.0
21	35.50111344964636	36.0	34.0	39.0	30.0	40.0
22	35.81177224994603	36.0	34.0	40.0	31.0	41.0
23	36.15392746942974	36.0	35.0	40.0	32.0	41.0
24	35.83259367897096	36.0	35.0	40.0	31.0	41.0
25	35.522419780833744	35.0	34.0	40.0	30.0	41.0
26	35.57063540436555	36.0	34.0	40.0	30.0	41.0
27	35.85503488755984	36.0	35.0	40.0	31.0	41.0
28	35.78591212398258	36.0	35.0	40.0	31.0	41.0
29	35.89858402219598	36.0	35.0	40.0	31.0	41.0
30	35.5146438865821	36.0	35.0	40.0	30.0	41.0
31	34.826310584993585	35.0	34.0	40.0	29.0	41.0
32	34.676871833453966	36.0	34.0	40.0	26.0	41.0
33	34.27266659682806	36.0	34.0	40.0	23.0	41.0
34	33.83291985702132	36.0	33.0	40.0	20.0	41.0
35	33.488081406422616	36.0	33.0	40.0	18.0	41.0
36	33.055015364494054	35.0	33.0	40.0	15.0	41.0
37	32.942206169923686	35.0	33.0	40.0	15.0	41.0
38	32.78433519992889	35.0	32.0	40.0	15.0	41.0
39	32.59151714855307	35.0	32.0	40.0	12.0	41.0
40	32.395377795131616	35.0	32.0	39.0	12.0	40.0
41	32.0905203611291	35.0	31.0	39.0	10.0	40.0
42	32.236284649473674	35.0	32.0	39.0	10.0	40.0
43	32.006552924967934	35.0	31.0	39.0	10.0	40.0
44	31.835276116465405	35.0	31.0	39.0	10.0	40.0
45	31.810637372544537	35.0	31.0	39.0	10.0	40.0
46	31.644500844412278	35.0	31.0	39.0	10.0	40.0
47	31.592091729838863	35.0	31.0	38.0	10.0	40.0
48	31.600852507206074	35.0	31.0	38.0	10.0	40.0
49	31.585739590872727	35.0	31.0	38.0	10.0	40.0
50	31.707081000088884	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	12.0
13	29.0
14	53.0
15	136.0
16	373.0
17	773.0
18	1536.0
19	2751.0
20	4001.0
21	5789.0
22	8541.0
23	12330.0
24	17795.0
25	27557.0
26	38941.0
27	44241.0
28	41103.0
29	38364.0
30	40448.0
31	48651.0
32	63629.0
33	95725.0
34	150746.0
35	116001.0
36	118054.0
37	163769.0
38	179778.0
39	38914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.833739667060303	21.486959226950084	26.76421850596168	24.915082600027937
2	40.90820349700964	19.199982857795895	28.248606402295785	11.643207242898683
3	26.841120338272827	18.663257272738818	41.72698182926365	12.768640559724709
4	24.395737305245515	23.680605818191054	38.45178913819156	13.47186773837187
5	20.378906200398713	27.796639493098674	38.781379756961634	13.04307454954097
6	18.251130115678134	34.909066956179444	36.83081914339771	10.008983784744709
7	73.87424923494979	4.5970470966185415	19.160460553883663	2.3682431145480174
8	75.04325232054651	3.3800299671123644	18.95411920815715	2.6225985041839674
9	71.19125620611278	4.910368493898646	20.356367376480897	3.5420079235076756
10	42.30116630477569	21.026103767475522	25.576406613081403	11.096323314667378
11	36.93978324635252	19.39251520576994	31.07714944192602	12.590552105951518
12	33.03120198595609	19.412196995670005	34.27671009358374	13.279890924790166
13	20.508980610262466	30.65708607926047	34.81605462649042	14.017878683986643
14	15.550677434510431	32.15187040493696	38.053788427107534	14.243663733445075
15	14.557699389229617	20.057648597513744	50.65267354894417	14.731978464312471
16	16.82189884829784	17.009352024684773	48.49989841656826	17.668850710449124
17	17.245057331149287	17.837574441608574	37.20405889299455	27.713309334247583
18	20.58858075247927	20.584930097901033	41.1916053991594	17.6348837504603
19	25.08333015885109	21.29434751691999	35.49952065318146	18.122801671047455
20	25.850919964953718	21.391089863243305	34.78835726892944	17.969632902873542
21	20.646991225731085	23.974483511739237	37.668009472655015	17.71051578987467
22	20.020348431170877	20.78198608306985	35.733241908244764	23.464423577514506
23	18.167006336266557	24.856513402664028	35.2490540042919	21.72742625677752
24	18.569689408657446	21.363313143626275	43.81841009231394	16.248587355402332
25	17.78106865770193	20.882458446027453	41.49389547064874	19.842577425621883
26	16.017723134356785	27.348402600535852	37.140172437875385	19.49370182723198
27	17.096729648394344	27.008415552423397	37.96442675199675	17.930428047185504
28	15.400841872690565	24.01567241882849	43.65294020545249	16.93054550302846
29	16.624922225185074	21.844167841225097	42.35156121036659	19.179348723223242
30	18.65000380937869	24.53033535230404	39.715471156654345	17.10418968166292
31	25.55442332355593	21.957258771094434	34.95557312102396	17.532744784325676
32	25.62775386334489	22.534459004736327	35.33301905959138	16.5047680723274
33	24.63556943862456	23.25720924917146	33.82585425317131	18.28136705903267
34	19.341247317562505	24.388118547864842	34.858592688532504	21.41204144604015
35	20.202881160082793	24.265345447157568	35.28397330895331	20.24780008380633
36	26.541211128464948	23.55180120122408	31.38428059883433	18.522707071476642
37	20.280259164730232	28.917549172729927	32.0367954236664	18.76539623887344
38	21.033405076632	28.939215014031213	29.465464807689866	20.56191510164692
39	21.29426815486394	26.855008698081345	30.868903406854344	20.981819740200372
40	23.606561019897654	23.858694271964243	30.42288865186088	22.111856056277222
41	18.817616471753457	22.834130128376064	32.03195433824743	26.31629906162305
42	21.91964115652737	24.00154597285183	29.274995873173086	24.80381699744772
43	21.31998146102371	24.646203954135082	29.966636191637143	24.06717839320407
44	19.90995581120719	27.191583177783702	31.34031401978337	21.558146991225733
45	18.67666946021104	30.947471842342516	28.50788223940675	21.867976458039692
46	21.150146661079578	27.645851586606224	30.131867992330452	21.072133759983746
47	20.58635861490991	25.569264028037026	31.193335491981255	22.651041865071807
48	21.38648686399248	24.096383629829976	32.96334742803449	21.553782078143055
49	21.191891102561172	23.203243051058372	33.72808020011936	21.876785646261094
50	19.63909311391312	27.927507523522916	31.345948725762828	21.08745063680114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	603.0
1	1301.0
2	1999.0
3	23889.0
4	45779.0
5	31470.0
6	17161.0
7	17228.5
8	17296.0
9	18106.0
10	18916.0
11	18864.5
12	18813.0
13	17859.0
14	16905.0
15	15763.0
16	14621.0
17	13680.5
18	12740.0
19	12031.0
20	11322.0
21	10445.5
22	9569.0
23	9420.5
24	9272.0
25	9790.0
26	10308.0
27	10803.0
28	11298.0
29	11829.5
30	12361.0
31	14484.5
32	16608.0
33	19896.0
34	23184.0
35	27380.5
36	31577.0
37	33630.5
38	35684.0
39	42884.0
40	50084.0
41	64866.0
42	79648.0
43	92447.0
44	105246.0
45	105081.5
46	104917.0
47	100659.0
48	96401.0
49	96375.5
50	96350.0
51	87605.0
52	78860.0
53	71567.0
54	64274.0
55	59211.0
56	54148.0
57	50962.0
58	47776.0
59	43959.0
60	40142.0
61	36310.0
62	32478.0
63	28396.0
64	24314.0
65	20823.5
66	17333.0
67	14183.0
68	11033.0
69	9502.0
70	7971.0
71	6808.0
72	5645.0
73	4443.5
74	3242.0
75	2539.0
76	1836.0
77	1442.5
78	1049.0
79	823.0
80	597.0
81	460.5
82	324.0
83	257.5
84	191.0
85	134.5
86	78.0
87	60.5
88	43.0
89	33.5
90	24.0
91	21.0
92	18.0
93	12.0
94	6.0
95	5.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1260048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.72135759347854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.8070290347742	22.34723504193917
2	7.040497742884303	4.044253066189172
3	3.112165202882189	2.6815682904588014
4	1.8742764035286106	2.1532705125905642
5	1.3673403490668485	1.9635935558770368
6	1.0222585160940632	1.7616391436229795
7	0.7944531438060086	1.597244098416092
8	0.6383275213171007	1.4666906401205797
9	0.5240668351172234	1.3546719876855897
>10	4.649904791672407	28.57149490828739
>50	0.8771232840965646	17.739033176869377
>100	0.2906197100275479	11.58872606102895
>500	0.0013839033807414892	0.2168628176116347
>1k	2.767806761482979E-4	0.09173742357911525
>5k	0.0	0.0
>10k+	2.767806761482979E-4	2.4219792757234875
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30467	2.4179237616344773	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03563356316584765	0.0
2	0.0	0.0	0.0	0.14920066537147791	0.0
3	0.0	0.0	0.0	0.20570644927812273	0.0
4	0.0	0.0	0.0	0.3041947608345079	0.0
5	0.0	0.0	0.0	0.5348208957119094	0.0
6	0.0	0.0	0.0	0.744971620128757	0.0
7	0.0	0.0	0.0	0.8979816641905706	0.0
8	0.0	0.0	0.0	1.268761190049903	0.0
9	0.0	0.0	0.0	1.4535160565311798	0.0
10	0.0	0.0	0.0	1.785090726702475	0.0
11	0.0	0.0	0.0	2.0321448071819486	0.0
12	7.936205604865846E-5	0.0	0.0	2.2789608014932763	0.0
13	7.936205604865846E-5	0.0	0.0	2.372925475854888	0.0
14	7.936205604865846E-5	0.0	0.0	2.4084003149086386	0.0
15	7.936205604865846E-5	0.0	0.0	2.471413787411273	0.0
16	7.936205604865846E-5	0.0	0.0	2.5905362335403095	0.0
17	7.936205604865846E-5	0.0	0.0	2.7264040734956128	0.0
18	7.936205604865846E-5	0.0	0.0	2.939015021649969	0.0
19	7.936205604865846E-5	0.0	0.0	3.028932231153099	0.0
20	1.5872411209731692E-4	0.0	0.0	3.1313092834558685	0.0
21	1.5872411209731692E-4	0.0	0.0	3.2495587469683693	0.0
22	1.5872411209731692E-4	0.0	0.0	3.3705065203865248	0.0
23	1.5872411209731692E-4	0.0	0.0	3.4963747412796975	0.0
24	1.5872411209731692E-4	0.0	0.0	3.5919266567622823	0.0
25	1.5872411209731692E-4	0.0	0.0	3.6754155397254706	0.0
26	1.5872411209731692E-4	0.0	0.0	3.7637455081076276	0.0
27	1.5872411209731692E-4	0.0	0.0	3.86120211293538	0.0
28	1.5872411209731692E-4	0.0	0.0	3.946754409355834	0.0
29	1.5872411209731692E-4	0.0	0.0	4.04754422053763	0.0
30	1.5872411209731692E-4	0.0	0.0	4.176904371896944	0.0
31	1.5872411209731692E-4	0.0	0.0	4.29007466382233	0.0
32	1.5872411209731692E-4	0.0	0.0	4.39483257780656	0.0
33	1.5872411209731692E-4	0.0	0.0	4.498876233286351	0.0
34	2.380861681459754E-4	0.0	0.0	4.613395680164565	0.0
35	2.380861681459754E-4	0.0	0.0	4.751247571521085	0.0
36	2.380861681459754E-4	0.0	0.0	4.862513174101304	0.0
37	3.1744822419463385E-4	0.0	0.0	4.970763018551674	0.0
38	3.968102802432923E-4	0.0	0.0	5.104726169161809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.5279114E-6	44.000004	1
TAGCACG	30	2.5279114E-6	44.000004	1
TAATGCG	30	2.5279114E-6	44.000004	1
TACGTAG	30	2.5279114E-6	44.000004	1
CTAGCGT	30	2.5279114E-6	44.000004	18
GGTACGA	20	7.8567717E-4	44.0	26
TCGATTG	25	4.443084E-5	44.0	1
CGCTATC	20	7.8567717E-4	44.0	29
CGAATCC	20	7.8567717E-4	44.0	20
CTACCGT	20	7.8567717E-4	44.0	19
TATCGTA	20	7.8567717E-4	44.0	26
TGCACGG	75	0.0	44.0	2
TATCGCG	35	1.4462421E-7	43.999996	1
CGTTTTT	18710	0.0	43.435596	1
ACGGGTA	130	0.0	42.30769	5
GCGATAT	75	0.0	41.066666	9
TTGTACG	60	3.6379788E-12	40.333336	1
ACCCGCT	325	0.0	39.938465	34
TACGGGA	375	0.0	39.893333	4
TCACGAC	160	0.0	39.875	25
>>END_MODULE