FastQCFastQC Report
Thu 26 May 2016
SRR1546028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546028_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1259262
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307502.4419064499683145No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA207.856768E-444.00000443
CCGAAAC207.856768E-444.00000444
TCTATCG207.856768E-444.0000041
ATTACGA351.4462239E-744.038
TAACGCG254.4430824E-544.01
CGTTCGA302.5279114E-644.014
GTGCGTA254.4430824E-544.016
CGTTTTT185300.043.382621
ACGATAG650.040.6153871
GTTTTTT207350.039.755972
TTACGGG3900.039.487183
GGGCGAT14250.039.2140357
ACGGGTA1350.039.111115
CTAACGG404.1249587E-738.5000042
TGCGTAG1200.038.51
TATGGGC4100.038.097564
GCGATAC1100.038.09
TGGGCGA4650.037.849466
GCGAGAC357.2907624E-637.71428721
TATCGCG357.2907624E-637.7142871
Produced by FastQC (version 0.11.5)