FastQCFastQC Report
Thu 26 May 2016
SRR1546027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546027_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1237863
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309102.497045311153173No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTCGA207.8567164E-444.024
CAATCGA207.8567164E-444.016
CGTTTTT176500.043.4764861
CGTAAGG1500.041.0666662
ACGGGTA1350.040.7407465
GTTTTTT198800.039.6287732
CTAACGG1000.039.62
ATTACGA452.3512257E-839.11110738
TTGTACG452.3512257E-839.1111071
TATCGCG452.3512257E-839.1111071
TAGGGTA4850.039.0103075
GACCGAT3950.038.987349
ACCCGCT4300.038.8837234
TCACGAC1700.038.82353225
CGACGGT1650.038.66666828
CGGTCTA1650.038.66666831
TATGGGC3700.038.648654
GAACGGG3700.038.648653
TCTAGCG404.124886E-738.51
TAATGCG404.124886E-738.51
Produced by FastQC (version 0.11.5)