##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546027_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1237863
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.986017030963847	31.0	28.0	33.0	23.0	33.0
2	28.81062201552191	31.0	28.0	33.0	16.0	33.0
3	28.145280212753754	31.0	27.0	33.0	16.0	34.0
4	32.00170455050357	35.0	32.0	35.0	25.0	37.0
5	33.6505639153929	35.0	33.0	35.0	28.0	37.0
6	33.58019102275454	35.0	33.0	37.0	28.0	37.0
7	34.28691220272356	35.0	33.0	37.0	31.0	37.0
8	34.7830284934601	35.0	35.0	37.0	32.0	37.0
9	36.68810361081962	39.0	37.0	39.0	32.0	39.0
10	36.41097601269284	39.0	35.0	39.0	32.0	39.0
11	36.5024102020983	39.0	35.0	39.0	32.0	39.0
12	35.344354746850016	37.0	34.0	39.0	30.0	39.0
13	35.46995911502323	37.0	35.0	39.0	30.0	39.0
14	36.20790749864888	38.0	35.0	40.0	30.0	41.0
15	36.694810330383895	38.0	35.0	40.0	31.0	41.0
16	36.29812184385509	38.0	34.0	40.0	31.0	41.0
17	35.91866951350836	37.0	34.0	40.0	30.0	41.0
18	35.53935047739532	37.0	34.0	39.0	29.0	40.0
19	34.93447982531185	37.0	33.0	39.0	27.0	40.0
20	35.36156182065382	35.0	34.0	39.0	30.0	40.0
21	35.47828879286318	36.0	34.0	39.0	30.0	40.0
22	35.80678394943544	36.0	34.0	40.0	31.0	41.0
23	36.16313194594232	36.0	35.0	40.0	32.0	41.0
24	35.73993325594189	36.0	35.0	40.0	31.0	41.0
25	35.45742460999319	35.0	34.0	40.0	30.0	41.0
26	35.54035301160145	36.0	34.0	40.0	30.0	41.0
27	35.87515338934922	36.0	35.0	40.0	31.0	41.0
28	35.808277652696624	36.0	35.0	40.0	31.0	41.0
29	35.91261957098645	36.0	35.0	40.0	31.0	41.0
30	35.56037865256494	36.0	35.0	40.0	30.0	41.0
31	34.84077155549524	35.0	34.0	40.0	29.0	41.0
32	34.73931363971619	36.0	34.0	40.0	27.0	41.0
33	34.353407444927264	36.0	34.0	40.0	23.0	41.0
34	33.84789189110588	36.0	33.0	40.0	20.0	41.0
35	33.498329782859656	36.0	33.0	40.0	18.0	41.0
36	33.095026670964394	35.0	33.0	40.0	15.0	41.0
37	32.999596078079726	36.0	33.0	40.0	15.0	41.0
38	32.851015015393465	35.0	33.0	40.0	15.0	41.0
39	32.61249427440678	35.0	32.0	40.0	12.0	41.0
40	32.440920360330665	35.0	32.0	40.0	12.0	40.0
41	32.11628831300394	35.0	31.0	39.0	10.0	40.0
42	32.3107791411489	35.0	32.0	39.0	10.0	40.0
43	32.09611079739842	35.0	31.0	39.0	10.0	40.0
44	31.91527172231499	35.0	31.0	39.0	10.0	40.0
45	31.88567232399708	35.0	31.0	39.0	10.0	40.0
46	31.70446325643468	35.0	31.0	39.0	10.0	40.0
47	31.677081389459094	35.0	31.0	39.0	10.0	40.0
48	31.683686320699465	35.0	31.0	38.0	10.0	40.0
49	31.72593332218509	35.0	31.0	39.0	10.0	40.0
50	31.794887641039438	35.0	31.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	1.0
11	7.0
12	9.0
13	24.0
14	48.0
15	143.0
16	295.0
17	755.0
18	1363.0
19	2462.0
20	3924.0
21	5738.0
22	8265.0
23	11923.0
24	17583.0
25	27164.0
26	38637.0
27	42737.0
28	40125.0
29	37613.0
30	39782.0
31	46983.0
32	61762.0
33	93612.0
34	148579.0
35	113775.0
36	115463.0
37	162391.0
38	178083.0
39	38615.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.928020305962775	21.40285314287607	26.613930620755283	25.055195930405873
2	41.06044045261875	19.183382975337334	28.159255103351498	11.596921468692416
3	26.83245237962521	18.705624128033556	41.68393432875852	12.777989163582722
4	24.282089374995454	23.702057497477508	38.427111885564074	13.588741241962964
5	20.316545530482777	27.833128544919756	38.849775782942054	13.000550141655417
6	18.27900179583686	34.859673485676524	36.893581923039946	9.967742795446668
7	73.78675992415961	4.628218146919328	19.24211322254563	2.342908706375423
8	75.03116257614938	3.396337074458159	19.012847140596335	2.559653208796127
9	71.25433105279018	4.946589404481756	20.38448519747339	3.4145943452546854
10	42.26954032877629	21.039969689699102	25.618667009192453	11.071822972332155
11	36.97784003560975	19.327340747724104	31.146742410105155	12.548076806560985
12	33.01100364095219	19.40489375641731	34.34952010036652	13.234582502263983
13	20.570773987105195	30.639739615773316	34.74899887952059	14.040487517600898
14	15.542026864039075	32.15404289489224	38.03627703550393	14.26765320556475
15	14.605897421604814	20.01505820918793	50.71280101271304	14.666243356494215
16	16.78546010342017	16.974657130877972	48.56821796919368	17.67166479650818
17	17.21232478876903	17.736777010056848	37.34936741788065	27.701530783293464
18	20.592343417647996	20.59403988971316	41.17394251221661	17.639674180422226
19	25.080885364535494	21.296136971538854	35.55660036692267	18.06637729700298
20	25.92144688063219	21.235710252265395	34.80304363245367	18.039799234648747
21	20.652770136921454	23.903049045007403	37.7119277335214	17.732253084549747
22	20.051572750780984	20.754962382751565	35.73077149894617	23.462693367521283
23	18.14498050268891	24.82075964787703	35.28548797403267	21.7487718754014
24	18.55245693586447	21.316656205088933	43.800727544162804	16.330159314883797
25	17.77910802730189	20.86507149821911	41.54490440379913	19.810916070679873
26	16.095157541666566	27.258590005517576	37.19571551940724	19.450536933408625
27	17.028863452579163	27.02875843287989	38.01971623677257	17.92266187776838
28	15.500261337482419	23.88495334297899	43.605471687900845	17.00931363163775
29	16.582448946288885	21.817761739384732	42.488708362718654	19.111080951607732
30	18.5946263843414	24.4405883365122	39.76360873537701	17.201176543769385
31	25.60614542966386	21.908563387063026	35.019788134874375	17.46550304839873
32	25.646214484155355	22.505559985232615	35.377178249935575	16.47104728067646
33	24.64481125940431	23.323259520641624	33.813515712158775	18.21841350779529
34	19.318939171782336	24.387674564955898	34.897319008646356	21.396067254615414
35	20.080251207120657	24.28394741582873	35.36667628000837	20.26912509704224
36	26.491946200831595	23.460189051615565	31.547109817483843	18.500754930068997
37	20.28043491081	28.903440849270073	32.162202117681844	18.653922122238082
38	20.9870559181428	29.012015061440565	29.57492064953876	20.426008370877877
39	21.26018792063419	26.864119858174934	30.919334368989137	20.956357852201737
40	23.62143468219019	23.841895266277447	30.494570077625717	22.042099973906645
41	18.762657903176684	22.92176113188616	32.1138930560167	26.20168790892045
42	21.788679361124778	24.131991989420477	29.368597332661206	24.710731316793538
43	21.469257906569627	24.602237889007103	29.960908436555577	23.96759576786769
44	19.891538885967186	27.255520198923467	31.450734047305716	21.402206867803624
45	18.715156685352095	31.02209210550764	28.5353064111295	21.727444798010765
46	21.16728587897045	27.653948781084818	30.2412302492279	20.937535090716825
47	20.604864997176588	25.54919243890479	31.25862878202192	22.587313781896704
48	21.42426100465076	24.120520606884607	32.98903028848911	21.466188099975522
49	21.21640278447615	23.283998309990686	33.851322803896714	21.648276101636448
50	19.5201730724644	28.085095038788623	31.34014022553384	21.054591663213134
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	592.0
1	1284.0
2	1976.0
3	24266.0
4	46556.0
5	32027.5
6	17499.0
7	17267.5
8	17036.0
9	17871.5
10	18707.0
11	18473.0
12	18239.0
13	17471.0
14	16703.0
15	15466.5
16	14230.0
17	13219.5
18	12209.0
19	11526.5
20	10844.0
21	10065.0
22	9286.0
23	9071.5
24	8857.0
25	9551.0
26	10245.0
27	10640.0
28	11035.0
29	11588.5
30	12142.0
31	14086.0
32	16030.0
33	19413.5
34	22797.0
35	26921.0
36	31045.0
37	33129.5
38	35214.0
39	42129.5
40	49045.0
41	63410.5
42	77776.0
43	90525.5
44	103275.0
45	103017.0
46	102759.0
47	98489.5
48	94220.0
49	94093.0
50	93966.0
51	85551.0
52	77136.0
53	70048.0
54	62960.0
55	58376.5
56	53793.0
57	50584.5
58	47376.0
59	43469.0
60	39562.0
61	35521.0
62	31480.0
63	27875.0
64	24270.0
65	20739.5
66	17209.0
67	14088.0
68	10967.0
69	9478.5
70	7990.0
71	6750.0
72	5510.0
73	4357.5
74	3205.0
75	2513.0
76	1821.0
77	1432.5
78	1044.0
79	800.5
80	557.0
81	431.0
82	305.0
83	242.0
84	179.0
85	140.0
86	101.0
87	78.0
88	55.0
89	44.0
90	33.0
91	25.5
92	18.0
93	11.5
94	5.0
95	3.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1237863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.358322801910717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47558855139816	21.970777494085677
2	7.184429332093501	4.074767322940466
3	3.0549690608680473	2.5990139633383835
4	1.935384752639231	2.1953706224495777
5	1.3769693493257642	1.9524270648258493
6	1.0114094932223854	1.72091261362304
7	0.8105255796627014	1.6089602219096486
8	0.6529778039675573	1.4813884277915814
9	0.5643566726469724	1.440378782850159
>10	4.72695473765805	28.6435419860175
>50	0.9228718132767899	18.391393283600486
>100	0.2821307176186078	11.178962094765701
>500	8.592813733791458E-4	0.13467258953012456
>1k	2.864271244597153E-4	0.0967400808989013
>5k	0.0	0.0
>10k+	2.864271244597153E-4	2.510693451372829
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30910	2.497045311153173	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.031182772245393876	0.0
2	0.0	0.0	0.0	0.1368487465898892	0.0
3	0.0	0.0	0.0	0.18879310553752718	0.0
4	0.0	0.0	0.0	0.28549201325187035	0.0
5	0.0	0.0	0.0	0.5014286718320202	0.0
6	0.0	0.0	0.0	0.6972500187823693	0.0
7	0.0	0.0	0.0	0.8456509322921842	0.0
8	0.0	0.0	0.0	1.1845414234046903	0.0
9	0.0	0.0	0.0	1.3632364809352893	0.0
10	0.0	0.0	0.0	1.6807998946571632	0.0
11	0.0	0.0	0.0	1.9222644185988271	0.0
12	0.0	0.0	0.0	2.1541156008378954	0.0
13	2.4235315216627364E-4	0.0	0.0	2.2514607836246823	0.0
14	2.4235315216627364E-4	0.0	0.0	2.287894540833679	0.0
15	2.4235315216627364E-4	0.0	0.0	2.3395965466291506	0.0
16	2.4235315216627364E-4	0.0	0.0	2.44800919003153	0.0
17	2.4235315216627364E-4	0.0	0.0	2.574759888614491	0.0
18	2.4235315216627364E-4	0.0	0.0	2.780033008499325	0.0
19	2.4235315216627364E-4	0.0	0.0	2.8638064147648	0.0
20	2.4235315216627364E-4	0.0	0.0	2.961393950703753	0.0
21	2.4235315216627364E-4	0.0	0.0	3.0697258097220774	0.0
22	2.4235315216627364E-4	0.0	0.0	3.1884788542835514	0.0
23	2.4235315216627364E-4	0.0	0.0	3.314502493410014	0.0
24	3.231375362216982E-4	0.0	0.0	3.4101512041316364	0.0
25	4.0392192027712275E-4	0.0	0.0	3.489562253658119	0.0
26	4.0392192027712275E-4	0.0	0.0	3.5718815410105966	0.0
27	4.0392192027712275E-4	0.0	0.0	3.6631678949932263	0.0
28	4.847063043325473E-4	0.0	0.0	3.749284048396309	0.0
29	4.847063043325473E-4	0.0	0.0	3.847517859407705	0.0
30	4.847063043325473E-4	0.0	0.0	3.977257580200717	0.0
31	5.654906883879718E-4	0.0	0.0	4.088901598965314	0.0
32	5.654906883879718E-4	0.0	0.0	4.19384051385331	0.0
33	5.654906883879718E-4	0.0	0.0	4.295467268995035	0.0
34	5.654906883879718E-4	0.0	0.0	4.4068689346074645	0.0
35	5.654906883879718E-4	0.0	0.0	4.547029840943626	0.0
36	5.654906883879718E-4	0.0	0.0	4.6551193468097845	0.0
37	5.654906883879718E-4	0.0	0.0	4.760704536770224	0.0
38	6.462750724433964E-4	0.0	0.0	4.892059945244345	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTCGA	20	7.8567164E-4	44.0	24
CAATCGA	20	7.8567164E-4	44.0	16
CGTTTTT	17650	0.0	43.476486	1
CGTAAGG	150	0.0	41.066666	2
ACGGGTA	135	0.0	40.740746	5
GTTTTTT	19880	0.0	39.628773	2
CTAACGG	100	0.0	39.6	2
ATTACGA	45	2.3512257E-8	39.111107	38
TTGTACG	45	2.3512257E-8	39.111107	1
TATCGCG	45	2.3512257E-8	39.111107	1
TAGGGTA	485	0.0	39.010307	5
GACCGAT	395	0.0	38.98734	9
ACCCGCT	430	0.0	38.88372	34
TCACGAC	170	0.0	38.823532	25
CGACGGT	165	0.0	38.666668	28
CGGTCTA	165	0.0	38.666668	31
TATGGGC	370	0.0	38.64865	4
GAACGGG	370	0.0	38.64865	3
TCTAGCG	40	4.124886E-7	38.5	1
TAATGCG	40	4.124886E-7	38.5	1
>>END_MODULE