FastQCFastQC Report
Thu 26 May 2016
SRR1546026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546026_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1087880
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158721.4589844468139868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA207.8562973E-444.014
GTTACGG551.8189894E-1244.02
CCGTAGG700.044.02
AACGCTA207.8562973E-444.037
TCGTCGG302.5275913E-644.02
AATGTCG254.442682E-544.011
TCTATGC207.8562973E-444.037
CGTTTTT165100.043.4803121
TGCGTAG1100.042.01
CGGGAAT13600.041.4117669
CGGAATT4000.040.159
ATATACG557.8216544E-1140.01
GCGAAAC501.3478711E-939.6000029
GTTTACG501.3478711E-939.6000021
CGACCCG4400.039.532
GTTTTTT184550.039.43432
ACCCGCT4400.039.034
GCGGAAT4800.038.9583328
TAACGAC404.1242674E-738.525
AGACGTG4300.038.37209324
Produced by FastQC (version 0.11.5)