##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546026_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1087880
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.997833400742728	28.0	25.0	30.0	16.0	33.0
2	26.249693900062507	30.0	25.0	31.0	10.0	33.0
3	26.426583814391293	30.0	25.0	31.0	16.0	33.0
4	27.8374912674192	32.0	19.0	35.0	10.0	37.0
5	31.190541236165753	33.0	30.0	35.0	19.0	37.0
6	29.96558719711733	33.0	28.0	35.0	17.0	36.0
7	29.97925966099202	32.0	28.0	35.0	17.0	35.0
8	32.489493326469834	35.0	32.0	35.0	28.0	35.0
9	27.231845424127663	31.0	23.0	34.0	10.0	35.0
10	31.582085340294885	34.0	30.0	37.0	17.0	39.0
11	33.83709140714049	35.0	32.0	37.0	27.0	39.0
12	33.2485035114167	35.0	31.0	39.0	25.0	39.0
13	33.3457293083796	35.0	32.0	39.0	24.0	39.0
14	33.16924385042468	36.0	31.0	39.0	22.0	40.0
15	32.09688017060705	34.0	27.0	38.0	18.0	40.0
16	30.657675478913117	34.0	27.0	38.0	17.0	40.0
17	30.824817075412728	33.0	27.0	38.0	17.0	40.0
18	31.755063977644593	34.0	29.0	38.0	18.0	39.0
19	31.38532650659999	34.0	28.0	37.0	18.0	39.0
20	32.0004871860867	34.0	30.0	37.0	22.0	39.0
21	32.816814354524396	34.0	31.0	37.0	24.0	39.0
22	33.15550060668456	35.0	31.0	37.0	25.0	39.0
23	34.440074272897746	35.0	33.0	38.0	29.0	40.0
24	34.07233242637056	35.0	33.0	38.0	27.0	40.0
25	32.92530242306137	35.0	31.0	38.0	23.0	39.0
26	26.46325789609148	33.0	20.0	36.0	0.0	39.0
27	29.731750744567414	34.0	24.0	37.0	15.0	39.0
28	31.26087252270471	34.0	28.0	37.0	20.0	39.0
29	32.54343953377211	35.0	30.0	37.0	23.0	39.0
30	32.3349385961687	35.0	30.0	37.0	22.0	39.0
31	31.21241772989668	34.0	29.0	36.0	19.0	39.0
32	31.414777365150567	34.0	30.0	37.0	18.0	39.0
33	31.082856565062322	34.0	29.0	37.0	18.0	39.0
34	30.942012905835202	34.0	29.0	37.0	15.0	39.0
35	30.07668584770379	34.0	27.0	37.0	10.0	39.0
36	29.781109129683422	34.0	26.0	37.0	10.0	39.0
37	29.84712100599331	34.0	27.0	37.0	10.0	39.0
38	29.594947052983784	33.0	26.0	37.0	10.0	39.0
39	29.477861528845093	33.0	26.0	37.0	10.0	39.0
40	29.366740817001876	33.0	26.0	36.0	10.0	38.0
41	28.76934312608008	33.0	24.0	36.0	9.0	38.0
42	28.917426554399384	33.0	24.0	36.0	8.0	38.0
43	28.489768172960254	33.0	24.0	35.0	8.0	38.0
44	28.252612420487555	32.0	23.0	35.0	8.0	38.0
45	27.78818343934993	31.0	23.0	35.0	8.0	38.0
46	27.383034893554438	31.0	23.0	35.0	8.0	38.0
47	27.443089311321103	31.0	23.0	35.0	8.0	38.0
48	27.506529212780823	31.0	23.0	35.0	8.0	37.0
49	27.338838842519394	31.0	23.0	35.0	8.0	37.0
50	29.09708056035592	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	17.0
12	39.0
13	123.0
14	303.0
15	770.0
16	1534.0
17	2833.0
18	4727.0
19	7713.0
20	11386.0
21	16248.0
22	22113.0
23	29646.0
24	38107.0
25	47470.0
26	55655.0
27	60529.0
28	65738.0
29	75984.0
30	90500.0
31	106306.0
32	116118.0
33	114772.0
34	99975.0
35	75196.0
36	36504.0
37	7356.0
38	207.0
39	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.108835533330883	22.342813545611648	26.388112659484502	26.160238261572964
2	40.76699635989264	16.724914512630072	29.81073280141192	12.697356326065377
3	28.092344743905578	14.580376512115306	43.21469279699967	14.112585946979447
4	25.514027282420855	23.66538588814943	37.33536787145641	13.485218957973306
5	19.767713350737214	27.962091407140495	39.45058278486598	12.819612457256316
6	18.229492223406993	33.5402801779608	37.97064014413355	10.259587454498657
7	75.91848365628562	3.3282163473912565	19.13234915615693	1.6209508401661947
8	77.69147332426371	2.140585358679266	18.537890208478878	1.6300511085781522
9	65.71083207706732	3.239787476559915	18.564179872780088	12.485200573592675
10	43.329135566422764	20.841912710960766	25.578648380336066	10.250303342280398
11	38.034342023017246	18.831396845240285	31.297569584880687	11.836691546861786
12	33.65941096444461	19.33990881347207	34.46777218075523	12.532908041328087
13	20.037779902195098	31.439680847152257	34.9618524101923	13.560686840460345
14	14.871677023201087	32.5887046365408	38.52722726771335	14.012391072544766
15	13.16808839210207	18.413795639224915	50.88051991028423	17.537596058388793
16	15.01103062837813	15.24028385483693	48.657664448284734	21.0910210685002
17	16.36936059124168	16.819685994778837	38.073776519469064	28.737176894510423
18	19.99053204397544	20.192300621392064	42.26817296025297	17.54899437437953
19	25.644096775379637	20.557138654998713	36.171268889951094	17.627495679670552
20	26.425800639776448	20.736570209949626	35.293874324374016	17.543754825899917
21	20.470823987939845	23.725318969003933	38.24741699452146	17.556440048534768
22	19.775159024892453	20.318969003934257	35.908372246939	23.99749972423429
23	17.569217193072763	24.85917564437254	35.85257565172629	21.7190315108284
24	17.786704415928227	20.63150347464794	45.83704085009376	15.744751259330073
25	17.18930396734934	19.75705040997169	43.24171783652609	19.811927786152886
26	12.654704563003271	39.620822149501784	31.003143729087768	16.721329558407177
27	16.345920505938157	27.91686583079016	38.421149391477	17.316064271794684
28	14.891624076184872	23.795823068720814	44.98336213552965	16.329190719564657
29	15.88796558443946	21.516619480089716	43.62521601647241	18.970198918998417
30	18.15521932566092	24.61025113063941	40.25866823546715	16.975861308232528
31	26.23524653454425	21.59493694157444	35.26960694194213	16.900209581939187
32	25.952494760451522	21.921443541567083	35.74649777548994	16.379563922491453
33	24.787844247527303	22.9160385336618	34.24035739235945	18.05575982645145
34	18.87597896826856	23.83691215942935	35.002298047578776	22.284810824723316
35	19.712927896459167	23.85253888296503	35.576809942273044	20.857723278302753
36	26.73539360958929	22.971651285068205	31.264937309262052	19.02801779608045
37	19.631852777879914	29.36895613486782	31.548975989998894	19.450215097253373
38	20.350038607199323	29.614939147700113	29.29633783137846	20.7386844137221
39	20.72664264440931	27.032485200573593	30.657425451336547	21.583446703680554
40	23.47887634665588	23.318196859947786	30.393333823583486	22.809592969812847
41	18.15623046659558	22.24050446740449	31.970529837849764	27.632735228150164
42	21.22311284332831	23.58146119057249	28.3230687208148	26.872357245284405
43	20.850829135566425	24.087950876934954	29.828657572526378	25.232562414972236
44	19.211861602382616	26.987535389932717	31.337371768945104	22.463231238739567
45	18.06201051586572	31.87254108909071	27.86327536125308	22.202173033790494
46	20.323105489576058	27.665827113284553	29.675056072360924	22.336011324778468
47	20.027760414751626	25.060760377982866	30.553921388388428	24.357557818877083
48	20.604202669412068	23.68082876787881	33.429881972276355	22.28508659043277
49	20.79006875758356	23.09059822774571	33.367283156230464	22.75204985844027
50	19.150733536787147	28.508291355664227	31.055998823399637	21.28497628414899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	349.0
1	749.0
2	1149.0
3	14048.5
4	26948.0
5	21036.5
6	15125.0
7	14803.5
8	14482.0
9	15347.5
10	16213.0
11	16798.5
12	17384.0
13	17353.0
14	17322.0
15	16566.5
16	15811.0
17	14958.5
18	14106.0
19	13466.0
20	12826.0
21	12047.5
22	11269.0
23	10725.5
24	10182.0
25	10395.5
26	10609.0
27	10788.5
28	10968.0
29	11303.5
30	11639.0
31	13522.5
32	15406.0
33	18088.0
34	20770.0
35	23738.0
36	26706.0
37	28522.0
38	30338.0
39	36361.5
40	42385.0
41	54078.5
42	65772.0
43	76637.5
44	87503.0
45	88171.5
46	88840.0
47	85102.5
48	81365.0
49	79832.5
50	78300.0
51	72688.0
52	67076.0
53	61454.0
54	55832.0
55	51891.5
56	47951.0
57	45148.5
58	42346.0
59	38939.0
60	35532.0
61	31857.0
62	28182.0
63	25010.0
64	21838.0
65	18633.5
66	15429.0
67	12816.0
68	10203.0
69	8805.5
70	7408.0
71	6274.0
72	5140.0
73	4085.0
74	3030.0
75	2459.5
76	1889.0
77	1473.0
78	1057.0
79	811.5
80	566.0
81	441.0
82	316.0
83	234.5
84	153.0
85	110.5
86	68.0
87	58.5
88	49.0
89	36.5
90	24.0
91	17.5
92	11.0
93	10.5
94	10.0
95	6.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1087880.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.80204285177583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4995074651049	36.13646961236924
2	6.964450363599961	6.101143065309425
3	2.715002166646942	3.5676792373840076
4	1.5430758514259515	2.7035949827079997
5	0.9937071358453479	2.176320127320668
6	0.7211549115871766	1.8952835004066075
7	0.521819031906808	1.5999717717517927
8	0.410202327451996	1.4374159939960405
9	0.32980097186724644	1.30013606720578
>10	2.8589842434448425	25.979829376789944
>50	0.37357097296500497	11.1588977450298
>100	0.06781730326279606	4.209617701224482
>500	6.804411683027129E-4	0.15677279149568724
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.2681372276757094E-4	1.5768680270085853
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15872	1.4589844468139868	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024175460528734788	0.0
2	0.0	0.0	0.0	0.109846674265544	0.0
3	0.0	0.0	0.0	0.1500165459425672	0.0
4	0.0	0.0	0.0	0.2120638305695481	0.0
5	0.0	0.0	0.0	0.3535316395190646	0.0
6	0.0	0.0	0.0	0.4702724565209398	0.0
7	0.0	0.0	0.0	0.5469353237489429	0.0
8	0.0	0.0	0.0	0.7619406552193256	0.0
9	0.0	0.0	0.0	0.8937566643379784	0.0
10	0.0	0.0	0.0	1.0994778835901018	0.0
11	0.0	0.0	0.0	1.2689818730006985	0.0
12	0.0	0.0	0.0	1.4267198588079568	0.0
13	0.0	0.0	0.0	1.4899621281759017	0.0
14	0.0	0.0	0.0	1.5103687906754422	0.0
15	0.0	0.0	0.0	1.5483325366768395	0.0
16	0.0	0.0	0.0	1.6154355259771298	0.0
17	0.0	0.0	0.0	1.7138838842519395	0.0
18	0.0	0.0	0.0	1.8490090818840312	9.192190315108284E-5
19	0.0	0.0	0.0	1.9006691914549398	9.192190315108284E-5
20	0.0	0.0	0.0	1.9609699599220503	9.192190315108284E-5
21	0.0	0.0	0.0	2.0255910578372616	9.192190315108284E-5
22	0.0	0.0	0.0	2.094808250910027	9.192190315108284E-5
23	0.0	0.0	0.0	2.1664154134647204	9.192190315108284E-5
24	0.0	0.0	0.0	2.2196381953891975	9.192190315108284E-5
25	0.0	0.0	0.0	2.2641283965143213	9.192190315108284E-5
26	0.0	0.0	0.0	2.3101812699930138	9.192190315108284E-5
27	0.0	0.0	0.0	2.374710446005074	9.192190315108284E-5
28	0.0	0.0	0.0	2.4371254182446593	9.192190315108284E-5
29	0.0	0.0	0.0	2.512225613119094	9.192190315108284E-5
30	0.0	0.0	0.0	2.6055263448174433	9.192190315108284E-5
31	0.0	0.0	0.0	2.6856822443651875	9.192190315108284E-5
32	0.0	0.0	0.0	2.760414751627018	9.192190315108284E-5
33	0.0	0.0	0.0	2.835514946501452	9.192190315108284E-5
34	0.0	0.0	0.0	2.923943817332794	9.192190315108284E-5
35	0.0	0.0	0.0	3.0242306136706256	9.192190315108284E-5
36	0.0	0.0	0.0	3.1069603265066	9.192190315108284E-5
37	0.0	0.0	0.0	3.1917123212118983	9.192190315108284E-5
38	0.0	0.0	0.0	3.287954553811082	9.192190315108284E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	20	7.8562973E-4	44.0	14
GTTACGG	55	1.8189894E-12	44.0	2
CCGTAGG	70	0.0	44.0	2
AACGCTA	20	7.8562973E-4	44.0	37
TCGTCGG	30	2.5275913E-6	44.0	2
AATGTCG	25	4.442682E-5	44.0	11
TCTATGC	20	7.8562973E-4	44.0	37
CGTTTTT	16510	0.0	43.480312	1
TGCGTAG	110	0.0	42.0	1
CGGGAAT	1360	0.0	41.411766	9
CGGAATT	400	0.0	40.15	9
ATATACG	55	7.8216544E-11	40.0	1
GCGAAAC	50	1.3478711E-9	39.600002	9
GTTTACG	50	1.3478711E-9	39.600002	1
CGACCCG	440	0.0	39.5	32
GTTTTTT	18455	0.0	39.4343	2
ACCCGCT	440	0.0	39.0	34
GCGGAAT	480	0.0	38.958332	8
TAACGAC	40	4.1242674E-7	38.5	25
AGACGTG	430	0.0	38.372093	24
>>END_MODULE