FastQCFastQC Report
Thu 26 May 2016
SRR1546025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546025_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences741156
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152262.0543583267220398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC302.5264944E-644.00000417
ACGTAAG302.5264944E-644.0000041
TAGGACG302.5264944E-644.0000041
CGGGTAT551.8189894E-1244.06
CGTATTA207.85467E-444.039
CGTTCGA408.305506E-944.014
CCCGTAT207.85467E-444.037
CGGGCTA551.8189894E-1244.06
ACGCTAG207.85467E-444.01
CGTACAT207.85467E-444.035
TCGAATG454.783942E-1044.017
AGTTACG351.4451507E-743.9999961
CGTTTTT91950.043.1147351
ATGCGAG800.041.251
ACGTAGG557.8216544E-1140.02
CGGTCTA1000.039.631
ACGGGCT1400.039.285715
ATCGTAG452.349043E-839.111111
GTTCGAA452.349043E-839.1111115
CGTAAGG1350.039.111112
Produced by FastQC (version 0.11.5)