FastQCFastQC Report
Thu 26 May 2016
SRR1546024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546024_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences749180
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158042.1095063936570653No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA207.8547234E-444.0000049
TCGATTG207.8547234E-444.0000041
CGTAGAA207.8547234E-444.00000412
TCGTTAG254.441351E-544.01
AACGGTT254.441351E-544.010
ATACGAG254.441351E-544.01
CGTTTTT94950.043.3048971
TAGGGTA2850.041.684215
TACGGGA1400.040.8571434
GACACGA501.3460522E-939.625
GGCGATA2400.039.4166688
CGTGGGA950.039.368424
GTTTTTT107550.039.1929322
CCCGATC452.349043E-839.1111141
TCACGAC800.038.50000425
ACGGGAC1200.038.5000045
ATAGGGC2800.038.54
ACCCGCT2800.038.534
TATACTA3450.038.26086844
GTAGGGT3400.038.1764684
Produced by FastQC (version 0.11.5)