FastQCFastQC Report
Thu 26 May 2016
SRR1546023_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546023_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences734668
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156132.1251776312565673No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7750.10548982669722923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA302.5264653E-644.06
TGCTACG207.8546256E-444.01
ACGTATG254.4412653E-544.01
AGTACGG502.7284841E-1144.02
AATCGTA207.8546256E-444.013
TTGTACG351.4451325E-743.9999961
ATGACGG700.043.9999962
CGTTTTT92650.043.358881
CGTAAGG1350.040.740742
ACAACGA557.8216544E-1140.013
GTTTTTT105250.039.338722
TATGGGC2350.039.3191454
ATAGGGC2450.038.6122444
AATCGTT2400.038.522
CCAATCA2500.037.8441
TCGTTGA2450.037.71428724
TAATCGT2450.037.71428721
GCGTAAG1000.037.41
GTAGGGT2600.037.230774
GCGTTCG651.0913936E-1137.2307744
Produced by FastQC (version 0.11.5)