##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546023_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	734668
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.74151725677449	31.0	28.0	31.0	16.0	33.0
2	28.69213304513059	31.0	28.0	33.0	16.0	33.0
3	28.109213957869404	31.0	26.0	33.0	16.0	34.0
4	32.10588592398199	35.0	32.0	35.0	25.0	37.0
5	33.62287863361409	35.0	33.0	35.0	28.0	37.0
6	33.48473324004856	35.0	33.0	37.0	28.0	37.0
7	34.32472899323232	35.0	35.0	37.0	31.0	37.0
8	34.72895784218178	35.0	35.0	37.0	32.0	37.0
9	36.58825074727632	39.0	37.0	39.0	32.0	39.0
10	36.297349278857936	38.0	35.0	39.0	32.0	39.0
11	36.40319028459113	38.0	35.0	39.0	32.0	39.0
12	35.34306652800993	37.0	34.0	39.0	30.0	39.0
13	35.68077417282364	37.0	35.0	39.0	30.0	39.0
14	36.377687608552435	38.0	35.0	40.0	30.0	41.0
15	36.78590873700774	38.0	35.0	40.0	31.0	41.0
16	36.315686813635544	38.0	34.0	40.0	31.0	41.0
17	35.947008716862584	38.0	34.0	40.0	30.0	41.0
18	35.55759336189953	37.0	34.0	39.0	29.0	40.0
19	35.00463883005657	37.0	33.0	39.0	27.0	41.0
20	35.51912428471092	36.0	34.0	39.0	30.0	41.0
21	35.62128199404357	36.0	34.0	39.0	30.0	40.0
22	35.97370512939178	37.0	34.0	40.0	31.0	41.0
23	36.30794590209455	37.0	35.0	40.0	31.0	41.0
24	35.878595501641556	36.0	35.0	40.0	31.0	41.0
25	35.53825401405805	36.0	34.0	40.0	30.0	41.0
26	35.67812807962236	37.0	34.0	40.0	30.0	41.0
27	36.02134433512825	37.0	35.0	40.0	31.0	41.0
28	35.935682512372935	37.0	35.0	40.0	31.0	41.0
29	36.05173493333043	37.0	35.0	40.0	31.0	41.0
30	35.68238850746187	37.0	35.0	40.0	30.0	41.0
31	34.96962709686552	36.0	34.0	40.0	29.0	41.0
32	34.95476460115317	37.0	34.0	40.0	27.0	41.0
33	34.666356776122	37.0	34.0	40.0	24.0	41.0
34	34.25149591380052	37.0	34.0	40.0	22.0	41.0
35	33.94691071341068	37.0	33.0	40.0	20.0	41.0
36	33.546754452351266	36.0	33.0	40.0	18.0	41.0
37	33.48652452536384	36.0	33.0	40.0	18.0	41.0
38	33.31466730550398	36.0	33.0	40.0	17.0	41.0
39	33.02571773916926	36.0	33.0	40.0	15.0	41.0
40	32.88137907190731	36.0	32.0	40.0	15.0	40.0
41	32.568142344569246	35.0	32.0	39.0	15.0	40.0
42	32.761286730877075	36.0	32.0	40.0	15.0	40.0
43	32.54542732227346	35.0	32.0	39.0	13.0	40.0
44	32.399889473884805	35.0	32.0	39.0	12.0	40.0
45	32.360988909276024	35.0	31.0	39.0	12.0	40.0
46	32.16993798559349	35.0	31.0	39.0	12.0	40.0
47	32.1264652877218	35.0	31.0	39.0	12.0	40.0
48	32.09724664746525	35.0	31.0	39.0	12.0	40.0
49	32.139222070377365	35.0	31.0	39.0	12.0	40.0
50	32.233212825385074	35.0	31.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	4.0
12	11.0
13	12.0
14	47.0
15	88.0
16	203.0
17	413.0
18	802.0
19	1372.0
20	2251.0
21	3246.0
22	4526.0
23	6587.0
24	9561.0
25	14573.0
26	20399.0
27	23340.0
28	22526.0
29	21738.0
30	23263.0
31	27910.0
32	36364.0
33	53516.0
34	80615.0
35	69414.0
36	75380.0
37	103026.0
38	109999.0
39	23479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.859702613969844	22.205132114097797	26.2835729880708	22.651592283861554
2	39.65069936352202	19.115437177065015	28.91251558527117	12.32134787414179
3	28.67934903929394	18.5063185003294	39.76313110139546	13.0512013589812
4	25.622049687750113	24.640245661986093	35.29499039021708	14.442714260046715
5	20.574191335405924	28.530846586485325	36.85950660706605	14.035455471042702
6	18.823468559948168	36.125841876875	34.98410166224743	10.066587900929399
7	75.67418207952436	4.774265382458471	17.0226551312974	2.52889740671977
8	76.81864461226023	3.610474391153555	16.77927989241399	2.7916011041722246
9	72.8183342679959	5.311787092945385	18.26375995687848	3.6061186821802504
10	41.5119482541774	23.294603821045698	23.606853708069494	11.586594216707411
11	34.401797818878734	21.759896987482783	30.278166464307688	13.5601387293308
12	30.94989845753456	21.21420832267092	33.74394964800427	14.09194357179025
13	20.67015304872405	29.488149749274502	34.478158841816985	15.363538360184464
14	16.944524601588746	30.67712218308134	36.824116471657945	15.55423674367197
15	16.16675831804298	21.66134907196176	46.46479770454137	15.707094905453891
16	18.68925827720821	18.8082235785416	44.07936646212983	18.42315168212036
17	19.027370186261006	19.087125068738533	35.73015293983133	26.155351805169136
18	21.548372870466658	20.9406153527852	38.990128874539245	18.52088290220889
19	26.042239487768626	21.994560808419585	33.56141821884171	18.40178148497008
20	26.78883522897418	22.093244839846026	32.92439578149586	18.19352414968394
21	22.246239117533364	23.802180032341138	35.560688637588676	18.39089221253682
22	21.920105408157152	21.355224400681667	34.13310502158799	22.591565169573194
23	19.30069092433589	25.050090653193006	34.191770976822184	21.45744744564892
24	19.05173493333043	21.855586468990072	41.55142186674797	17.541256730931522
25	19.097605993455545	22.553861063773024	38.809230836241674	19.539302106529753
26	17.91094753004078	26.77672091339217	34.990907457518226	20.32142409904882
27	18.318886898571872	26.59609510690543	35.9714864401335	19.113531554389194
28	17.43073061573391	23.841789760817132	40.53259431471086	18.194885308738094
29	18.38953105348266	22.677045958174304	39.129239329874174	19.80418365846886
30	20.185580425443874	25.00272231810832	36.52044188667534	18.29125536977247
31	25.21166023292154	23.380220725552224	32.87267173743786	18.535447304088375
32	26.029308476754125	22.945602639559638	33.07616501603445	17.948923867651782
33	25.25957303162789	23.89991125242967	31.43174876270642	19.40876695323602
34	19.79615282004933	24.908257879749765	33.25066560677749	22.044923693423424
35	20.81402756074853	24.57940185226524	33.80452122591429	20.80204936107194
36	26.35557830203575	24.533122444423878	29.943457452890286	19.16784180065009
37	20.354228032254024	28.957433834058378	30.750352540195024	19.93798559349257
38	20.70145970696968	29.160382649033306	29.0766713672026	21.061486276794415
39	21.34746579407297	27.88429603576037	29.421044607904523	21.347193562262138
40	23.456581748490475	24.96392928506482	29.36632601392738	22.213162952517326
41	19.678140330053846	23.751817147337302	30.507521764933276	26.06252075767558
42	21.745196469697877	24.46914796887846	28.92762445077232	24.85803111065134
43	21.277910566405506	24.69782268997697	30.1816602873679	23.842606456249626
44	20.51511703245548	26.524089792940487	30.783973168832723	22.176820005771315
45	19.521743154731116	30.274355218956046	28.175175725633895	22.028725900678946
46	21.408037371982992	27.738379785154656	29.28247861619126	21.571104226671096
47	20.336805196360803	26.286703653895366	30.704753711880738	22.67173743786309
48	21.19746606630478	24.362569214937903	32.715866214398886	21.72409850435843
49	21.60703882570086	24.293830682702936	32.7452672499687	21.35386324162751
50	19.912259687368987	28.188787316175468	30.81092411810505	21.088028878350492
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	274.0
1	652.0
2	1030.0
3	12756.0
4	24482.0
5	17028.0
6	9574.0
7	9509.0
8	9444.0
9	9466.5
10	9489.0
11	9276.5
12	9064.0
13	8621.5
14	8179.0
15	7711.5
16	7244.0
17	6663.5
18	6083.0
19	5768.0
20	5453.0
21	5164.0
22	4875.0
23	4809.5
24	4744.0
25	5055.5
26	5367.0
27	5979.5
28	6592.0
29	7773.0
30	8954.0
31	10167.5
32	11381.0
33	12904.0
34	14427.0
35	16882.5
36	19338.0
37	20626.0
38	21914.0
39	26072.0
40	30230.0
41	37341.5
42	44453.0
43	49926.5
44	55400.0
45	55354.0
46	55308.0
47	53872.5
48	52437.0
49	52678.0
50	52919.0
51	50545.0
52	48171.0
53	44965.0
54	41759.0
55	38570.5
56	35382.0
57	33227.0
58	31072.0
59	28139.0
60	25206.0
61	23284.5
62	21363.0
63	18947.0
64	16531.0
65	14512.5
66	12494.0
67	10464.5
68	8435.0
69	7011.0
70	5587.0
71	4752.0
72	3917.0
73	3390.5
74	2864.0
75	2177.5
76	1491.0
77	1185.5
78	880.0
79	642.0
80	404.0
81	328.5
82	253.0
83	171.0
84	89.0
85	73.5
86	58.0
87	42.0
88	26.0
89	21.0
90	16.0
91	9.5
92	3.0
93	3.5
94	4.0
95	6.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	734668.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.54775564218343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96154516896875	24.81243844246876
2	8.199376070963744	5.5014132969411556
3	3.9813720021201275	4.006982851432699
4	2.498800998179661	3.3531666114150123
5	1.771201596142557	2.9709919170217876
6	1.307974507397259	2.632776549622109
7	0.9913532156198455	2.328037280289515
8	0.7961496435555974	2.136722695729169
9	0.6454022761750254	1.948661806682586
>10	5.373453727156383	37.098673726397294
>50	0.4415406887448294	9.372660074216434
>100	0.03101394843837789	1.5939395129835883
>500	4.080782689260249E-4	0.10609835287832009
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.080782689260249E-4	2.1374368819215634
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15613	2.1251776312565673	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	775	0.10548982669722923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.028720456042729506	0.0
2	0.0	0.0	0.0	0.10195081315641895	0.0
3	0.0	0.0	0.0	0.13366581911829561	0.0
4	0.0	0.0	0.0	0.1985931060016225	0.0
5	0.0	0.0	0.0	0.35580697675684797	0.0
6	0.0	0.0	0.0	0.5279935971078092	0.0
7	0.0	0.0	0.0	0.6481839415899426	0.0
8	0.0	0.0	0.0	0.9628839149112252	0.0
9	0.0	0.0	0.0	1.108119585989862	0.0
10	0.0	0.0	0.0	1.341013900156261	0.0
11	0.0	0.0	0.0	1.523953677035069	0.0
12	0.0	0.0	0.0	1.6855232567635994	0.0
13	0.0	0.0	0.0	1.7504505436469262	0.0
14	1.3611590541577964E-4	0.0	0.0	1.7763125656759244	0.0
15	1.3611590541577964E-4	0.0	0.0	1.8106137738407009	0.0
16	1.3611590541577964E-4	0.0	0.0	1.9009947350367786	0.0
17	2.722318108315593E-4	0.0	0.0	2.007029025355671	0.0
18	4.0834771624733893E-4	0.0	0.0	2.1695514164221117	0.0
19	4.0834771624733893E-4	0.0	0.0	2.241012266765396	0.0
20	4.0834771624733893E-4	0.0	0.0	2.3326182711102157	0.0
21	4.0834771624733893E-4	0.0	0.0	2.42504097088753	0.0
22	4.0834771624733893E-4	0.0	0.0	2.5182803660973394	0.0
23	4.0834771624733893E-4	0.0	0.0	2.6248591200378946	0.0
24	4.0834771624733893E-4	0.0	0.0	2.7096593291119255	0.0
25	4.0834771624733893E-4	0.0	0.0	2.780984063549794	0.0
26	4.0834771624733893E-4	0.0	0.0	2.8542144206634834	0.0
27	4.0834771624733893E-4	0.0	0.0	2.9345228048587932	0.0
28	4.0834771624733893E-4	0.0	0.0	3.0153756526757665	0.0
29	4.0834771624733893E-4	0.0	0.0	3.105348266155597	0.0
30	4.0834771624733893E-4	0.0	0.0	3.2252663788268987	0.0
31	5.444636216631186E-4	0.0	0.0	3.322044787577518	0.0
32	5.444636216631186E-4	0.0	0.0	3.41841484861189	0.0
33	5.444636216631186E-4	0.0	0.0	3.5173711118491617	0.0
34	5.444636216631186E-4	0.0	0.0	3.6220442431138964	0.0
35	5.444636216631186E-4	0.0	0.0	3.7479514556234923	0.0
36	5.444636216631186E-4	0.0	0.0	3.842279778076628	0.0
37	5.444636216631186E-4	0.0	0.0	3.946544561625115	0.0
38	5.444636216631186E-4	0.0	0.0	4.073676817283453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCTA	30	2.5264653E-6	44.0	6
TGCTACG	20	7.8546256E-4	44.0	1
ACGTATG	25	4.4412653E-5	44.0	1
AGTACGG	50	2.7284841E-11	44.0	2
AATCGTA	20	7.8546256E-4	44.0	13
TTGTACG	35	1.4451325E-7	43.999996	1
ATGACGG	70	0.0	43.999996	2
CGTTTTT	9265	0.0	43.35888	1
CGTAAGG	135	0.0	40.74074	2
ACAACGA	55	7.8216544E-11	40.0	13
GTTTTTT	10525	0.0	39.33872	2
TATGGGC	235	0.0	39.319145	4
ATAGGGC	245	0.0	38.612244	4
AATCGTT	240	0.0	38.5	22
CCAATCA	250	0.0	37.84	41
TCGTTGA	245	0.0	37.714287	24
TAATCGT	245	0.0	37.714287	21
GCGTAAG	100	0.0	37.4	1
GTAGGGT	260	0.0	37.23077	4
GCGTTCG	65	1.0913936E-11	37.23077	44
>>END_MODULE