FastQCFastQC Report
Thu 26 May 2016
SRR1546022_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546022_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences601745
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73041.2138031890584882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT207.853487E-444.00000421
GCTCGAA302.5256977E-644.00000421
GACGTAG254.4403023E-544.01
CTAACGG254.4403023E-544.02
TACGTTG254.4403023E-544.01
CGTTTTT75900.043.3043481
CGGTCTA950.041.6842131
CACGACG700.040.85714326
TACCGGG603.6379788E-1240.3333363
TATCGGG557.8216544E-1140.03
ATGCGAG1050.039.809521
CGCATGG950.039.3684232
GTTTTTT86000.039.0116232
CGGGAAT6250.038.7199979
AACGTTG404.1201747E-738.5000041
TAACGCC404.1201747E-738.50000412
ACGGGTA404.1201747E-738.5000045
TCGATAG404.1201747E-738.5000041
TGCCTAA404.1201747E-738.50000440
TCACGAC750.038.1333325
Produced by FastQC (version 0.11.5)