##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546022_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	601745
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.659817696864952	28.0	23.0	30.0	16.0	33.0
2	26.0711480776741	30.0	25.0	31.0	10.0	33.0
3	26.334625131908034	30.0	25.0	31.0	16.0	33.0
4	28.26431461831839	32.0	19.0	35.0	10.0	37.0
5	31.211368602979668	33.0	30.0	35.0	19.0	37.0
6	29.779956626145626	33.0	28.0	35.0	17.0	37.0
7	29.6519821519082	32.0	28.0	35.0	17.0	35.0
8	32.32633258273854	33.0	32.0	35.0	28.0	35.0
9	26.965553515193314	30.0	23.0	34.0	10.0	35.0
10	31.290297385104985	34.0	28.0	37.0	17.0	37.0
11	33.622373264422635	35.0	32.0	37.0	27.0	39.0
12	33.193847892379665	35.0	31.0	38.0	24.0	39.0
13	33.56603544690857	35.0	32.0	38.0	25.0	39.0
14	33.3019285577778	36.0	31.0	39.0	22.0	40.0
15	32.018399820521985	34.0	27.0	38.0	18.0	40.0
16	30.46910900796849	33.0	26.0	38.0	17.0	40.0
17	30.79323799948483	33.0	27.0	38.0	17.0	40.0
18	31.679241206823487	34.0	28.0	38.0	18.0	39.0
19	31.403144188983706	34.0	27.0	37.0	18.0	39.0
20	32.056233122003505	34.0	30.0	37.0	21.0	39.0
21	32.894310713009666	35.0	31.0	37.0	24.0	39.0
22	33.30330954141704	35.0	31.0	38.0	25.0	39.0
23	34.57263126407365	35.0	33.0	38.0	29.0	40.0
24	34.20697970070378	35.0	33.0	38.0	27.0	40.0
25	32.92429849853343	35.0	31.0	38.0	23.0	40.0
26	26.558831398682166	33.0	21.0	36.0	0.0	39.0
27	29.879084994474404	34.0	24.0	37.0	15.0	39.0
28	31.393096743637255	34.0	29.0	37.0	20.0	39.0
29	32.68455076485887	35.0	30.0	37.0	23.0	39.0
30	32.44278390348071	35.0	30.0	38.0	22.0	39.0
31	31.288703686777623	34.0	29.0	37.0	19.0	39.0
32	31.65102493581168	34.0	30.0	37.0	20.0	39.0
33	31.361521907120125	34.0	30.0	37.0	18.0	39.0
34	31.350811390206815	34.0	30.0	38.0	16.0	39.0
35	30.547677172224116	34.0	29.0	37.0	12.0	39.0
36	30.287028558608714	34.0	27.0	37.0	10.0	39.0
37	30.413685198879925	34.0	28.0	37.0	10.0	39.0
38	30.110530207978464	34.0	27.0	37.0	10.0	39.0
39	29.959055746204786	34.0	27.0	37.0	10.0	39.0
40	29.843305719199993	34.0	27.0	37.0	10.0	39.0
41	29.25383841992871	33.0	26.0	36.0	10.0	38.0
42	29.443805931083766	33.0	26.0	36.0	10.0	38.0
43	28.99476356263866	33.0	25.0	36.0	10.0	38.0
44	28.796340642631016	33.0	24.0	36.0	10.0	38.0
45	28.311791539605647	32.0	24.0	35.0	9.0	38.0
46	27.902272557312482	31.0	23.0	35.0	8.0	38.0
47	27.951346500594106	31.0	23.0	35.0	9.0	38.0
48	27.975114043323998	31.0	23.0	35.0	9.0	38.0
49	27.772725988583204	31.0	24.0	35.0	8.0	38.0
50	29.622996451985475	34.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	12.0
12	30.0
13	64.0
14	175.0
15	396.0
16	754.0
17	1417.0
18	2529.0
19	4071.0
20	5943.0
21	8321.0
22	11457.0
23	15040.0
24	19399.0
25	24605.0
26	29068.0
27	32497.0
28	36088.0
29	41686.0
30	49940.0
31	58582.0
32	65234.0
33	66406.0
34	58783.0
35	43704.0
36	21247.0
37	4180.0
38	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.938985783014402	23.61664824801203	26.018496206865034	23.425869762108533
2	39.28025991075954	16.705248901112597	30.61645713715943	13.398034050968432
3	30.19086157757854	14.428038454827211	41.04678892221788	14.334311045376364
4	27.0689411627849	24.591147412940696	33.83825374535726	14.501657678917148
5	19.795095929338842	29.041205161654855	37.238863638252084	13.924835270754224
6	18.845524266923697	34.93207255565065	35.85389159860073	10.368511578824917
7	78.22998113818976	3.4409924469667383	16.55568388603146	1.7733425288120381
8	79.89530448944319	2.333380418615859	15.950111758302935	1.8212033336380027
9	67.25423559813542	3.567624159735436	16.254393472318007	12.923746769811132
10	42.555567557686395	23.341614803612824	23.256861295066848	10.845956343633931
11	35.43378008957283	21.400427091209732	30.128542821294737	13.037249997922709
12	31.584475151434578	21.313679382462674	33.775270255673085	13.326575210429667
13	20.14973119843123	30.48965924104064	34.44249640628506	14.918113154243077
14	16.416089871955727	31.142261256844677	37.21593033593964	15.225718535259952
15	14.691605248070196	20.343500984636346	46.452899484000696	18.511994283292758
16	16.875919201655186	17.12818552709204	43.999700869969836	21.996194401282935
17	18.29828249507682	18.371403169116487	36.10582555733741	27.224488778469286
18	21.201173254451636	20.587125775868515	39.676108650674294	18.53559231900556
19	26.795071001836323	21.586884810010883	33.781252856276325	17.83679133187646
20	27.44567881743928	21.741102958894547	33.15241505953519	17.660803164130986
21	22.039402072306373	23.815569718069945	35.828631729387034	18.316396480236648
22	21.605497345220982	21.30121563120591	34.074566469185456	23.018720554387656
23	18.759607474927087	25.27116968150961	34.54785665024222	21.42136619332109
24	18.42242145759416	21.35721942018629	43.297908582539115	16.92245053968043
25	18.628821178406135	21.685763903314527	40.09721725980274	19.5881976584766
26	14.266674421889672	39.28840289491396	28.882998612369025	17.561924070827345
27	17.508413032098314	27.498525122767948	36.30474702739532	18.688314817738412
28	16.79365844336056	23.67780372084521	41.865241921411894	17.66329591438234
29	17.700188618102352	22.534296088874857	40.12048292881536	19.64503236420743
30	19.765847659722972	25.230787127437704	36.84617238198905	18.157192830850278
31	25.882392043141202	23.350588704517694	32.873559398083906	17.893459854257202
32	26.275914216154682	22.44090104612419	33.33737712818552	17.9458076095356
33	25.43269989779724	23.73630026007694	31.54475733076303	19.286242511362786
34	19.338756449991276	24.541790957963922	33.18964012995538	22.929812462089423
35	20.49722058346974	24.23784161064903	34.00078106174542	21.264156744135803
36	26.396230961619953	24.32641733624708	29.670873875146448	19.606477826986517
37	19.65583428196329	29.603569618359938	30.2945599880348	20.446036111641973
38	19.960115995978363	29.99626087462297	28.890476863123084	21.153146266275584
39	20.86099593681709	28.195996643096326	29.243948848764845	21.69905857132174
40	23.48586195149108	24.504898254243905	29.322221206657307	22.687018587607707
41	19.10576739316488	23.182909704276728	30.54300409641958	27.168318806138814
42	21.00042376754273	24.096751946422486	28.016352441648873	26.886471844385913
43	20.850526385761412	24.224214575941634	29.944910219445113	24.980348818851837
44	19.66763329981969	26.417003880381223	30.91758136752279	22.997781452276296
45	19.035638019426834	31.454519771664074	27.41127886397062	22.09856334493847
46	20.717413522339196	27.880414461275127	28.657820172996868	22.74435184338881
47	19.608472027187595	25.943879882674555	30.14133893925168	24.306309150886175
48	20.372583070902124	23.976933751007486	33.41731131957889	22.233171858511497
49	21.20798677180533	24.23634596049822	32.324489609386035	22.231177658310415
50	19.32413231518334	29.02392209324548	30.620113170861412	21.03183242070977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	359.5
2	589.0
3	6805.0
4	13021.0
5	10300.0
6	7579.0
7	7464.0
8	7349.0
9	7644.0
10	7939.0
11	8167.0
12	8395.0
13	8221.0
14	8047.0
15	7714.0
16	7381.0
17	6976.0
18	6571.0
19	6277.0
20	5983.0
21	5617.5
22	5252.0
23	5060.5
24	4869.0
25	5005.5
26	5142.0
27	5617.0
28	6092.0
29	6963.0
30	7834.0
31	8839.0
32	9844.0
33	11159.0
34	12474.0
35	14243.0
36	16012.0
37	17139.0
38	18266.0
39	21183.0
40	24100.0
41	29742.5
42	35385.0
43	40004.5
44	44624.0
45	45317.5
46	46011.0
47	44257.0
48	42503.0
49	41826.0
50	41149.0
51	39388.0
52	37627.0
53	35365.5
54	33104.0
55	31006.0
56	28908.0
57	27286.5
58	25665.0
59	23223.0
60	20781.0
61	19280.0
62	17779.0
63	15742.5
64	13706.0
65	12139.0
66	10572.0
67	8894.0
68	7216.0
69	6087.0
70	4958.0
71	4189.5
72	3421.0
73	2946.5
74	2472.0
75	1943.5
76	1415.0
77	1095.5
78	776.0
79	577.0
80	378.0
81	299.5
82	221.0
83	163.0
84	105.0
85	79.0
86	53.0
87	40.5
88	28.0
89	21.5
90	15.0
91	8.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	601745.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.13459682845115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.11426137648016	40.6663079114744
2	7.5227366339729125	7.542987363817034
3	3.088535540870473	4.645274523956504
4	1.8272993835357827	3.664436715137751
5	1.191885471362209	2.9877348786216404
6	0.8433601246805478	2.5368911899230935
7	0.6195372770677171	2.1742176124190506
8	0.5026657530344076	2.016075589427986
9	0.3994018627481532	1.8021466225269938
>10	2.78556043316662	26.899278259180658
>50	0.09572544115307043	3.1033337332823976
>100	0.008669473850883184	0.6385594465761868
>500	0.0	0.0
>1k	0.0	0.0
>5k	3.612280771201326E-4	1.3227561536564003
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7304	1.2138031890584882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019775818660728382	0.0
2	0.0	0.0	0.0	0.08043274144363476	0.0
3	0.0	0.0	0.0	0.10768681085842009	0.0
4	0.0	0.0	0.0	0.14806936493032763	0.0
5	0.0	0.0	0.0	0.2544267089880265	0.0
6	0.0	0.0	0.0	0.35380435234193885	0.0
7	0.0	0.0	0.0	0.4240999094300742	0.0
8	0.0	0.0	0.0	0.6100590781809571	0.0
9	0.0	0.0	0.0	0.715419322138115	0.0
10	0.0	0.0	0.0	0.8498616523610499	0.0
11	0.0	0.0	0.0	0.9754962650292067	0.0
12	0.0	0.0	0.0	1.0805241422861844	0.0
13	0.0	0.0	0.0	1.1229008965591738	0.0
14	0.0	0.0	0.0	1.1406825150188202	0.0
15	0.0	0.0	0.0	1.1647791007818926	0.0
16	0.0	0.0	0.0	1.2111442554570457	0.0
17	0.0	0.0	0.0	1.2982243309042867	0.0
18	0.0	0.0	0.0	1.4004270912097316	0.0
19	0.0	0.0	0.0	1.44164056203209	0.0
20	0.0	0.0	0.0	1.4916617504092264	0.0
21	0.0	0.0	0.0	1.5435109556373547	0.0
22	0.0	0.0	0.0	1.5960248942658435	0.0
23	0.0	0.0	0.0	1.6555185335981188	0.0
24	0.0	0.0	0.0	1.705041171924985	0.0
25	0.0	0.0	0.0	1.7406044088442778	0.0
26	0.0	0.0	0.0	1.7836458965176278	0.0
27	0.0	0.0	0.0	1.8381540353471986	0.0
28	0.0	0.0	0.0	1.8905017906255972	0.0
29	0.0	0.0	0.0	1.9549809304605772	0.0
30	0.0	0.0	0.0	2.0423933726079984	0.0
31	0.0	0.0	0.0	2.117508246848748	0.0
32	0.0	0.0	0.0	2.1884685373372443	0.0
33	0.0	0.0	0.0	2.263251044877814	0.0
34	0.0	0.0	0.0	2.3340451520162193	0.0
35	0.0	0.0	0.0	2.4319271452193205	0.0
36	0.0	0.0	0.0	2.5092024030112423	0.0
37	0.0	0.0	0.0	2.582821627101181	0.0
38	0.0	0.0	0.0	2.671563536049323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	20	7.853487E-4	44.000004	21
GCTCGAA	30	2.5256977E-6	44.000004	21
GACGTAG	25	4.4403023E-5	44.0	1
CTAACGG	25	4.4403023E-5	44.0	2
TACGTTG	25	4.4403023E-5	44.0	1
CGTTTTT	7590	0.0	43.304348	1
CGGTCTA	95	0.0	41.68421	31
CACGACG	70	0.0	40.857143	26
TACCGGG	60	3.6379788E-12	40.333336	3
TATCGGG	55	7.8216544E-11	40.0	3
ATGCGAG	105	0.0	39.80952	1
CGCATGG	95	0.0	39.368423	2
GTTTTTT	8600	0.0	39.011623	2
CGGGAAT	625	0.0	38.719997	9
AACGTTG	40	4.1201747E-7	38.500004	1
TAACGCC	40	4.1201747E-7	38.500004	12
ACGGGTA	40	4.1201747E-7	38.500004	5
TCGATAG	40	4.1201747E-7	38.500004	1
TGCCTAA	40	4.1201747E-7	38.500004	40
TCACGAC	75	0.0	38.13333	25
>>END_MODULE