FastQCFastQC Report
Thu 26 May 2016
SRR1546021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546021_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences227581
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36761.615249076153106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT207.843163E-444.028
GACGTTG207.843163E-444.01
ATTACGG207.843163E-444.02
TTCGTAG207.843163E-444.01
CACGACG207.843163E-444.026
AATTCGC207.843163E-444.013
TTCGGGC207.843163E-444.04
ACGTAGG207.843163E-444.02
ATAGTTA207.843163E-444.037
ACTCATG254.431557E-544.022
CGTTTTT25600.042.8828121
AATCCTA1050.041.9047613
TAGGGGT900.041.5555575
GGGCGAT3100.041.1612937
TAACGGG700.040.8571433
CGAGGGC603.6379788E-1240.3333324
AGGGCGA1700.040.117656
ATCCTAG1100.040.014
TAATCCT1050.039.8095212
CTTGTAG452.3366738E-839.111111
Produced by FastQC (version 0.11.5)